Liquid chromatography with tandem mass spectrometry for the simultaneous determination of baicalein, baicalin, oroxylin A and wogonin in rat plasma

Kim, Young Hoon; Jeong, Dong Won; Paek, In Bok; Ji, Hye Young; Kim, Youn‐Chul; Sohn, Dong Hwan; Lee, Hye Suk

doi:10.1016/j.jchromb.2006.07.021

Cited by 64 publications

(38 citation statements)

References 30 publications

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“…A bimodal phenomenon of 10 flavonoids was presented in Fig. 4, which was in agreement with the literature and was probably due to distribution, re-absorption and enterohepatic circulation (Kowalczyk et al, 2006;Kotani et al, 2006;Zhu et al, 2010;Tong et al, 2012;Kim et al, 2006;Walle et al, 2001;Lu et al, 2007). This is the first study on the pharmacokinetics of flavonoids, including scutellarin, scutellarein and apigenin from RS.…”

Section: Analytessupporting

confidence: 86%

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Cui

Qian

Huang

et al. 2014

Biomedical Chromatography

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Radix Scutellariae (RS) is a herbal medicine with various pharmacological activities to treat inflammation, respiratory and gastrointestinal infections, etc. In this study, a rapid, sensitive and selective UPLC-ESI-MS/MS method was developed for simultaneous determination of 10 flavonoids - scutellarin, scutellarein, chrysin, wogonin, baicalein, apigenin, wogonoside, oroxylin A-7-O-glucuronide, oroxylin A and baicalin - from RS aqueous extracts in rat plasma with propyl paraben as internal standard (IS). Chromatographic separation was achieved on a C18 column using gradient elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection was performed in multiple reaction monitoring mode using electrospray ionization in negative mode. The validated method showed good linearity over a wide concentration range (r >0.9935). The intra- and interday assay variabilities were <9.5% and <12.4% for all analytes, respectively. The extraction recovery ranged from 71.2 to 89.7% for each analyte and IS. This method was successfully applied to pharmacokinetic comparision after oral administration of crude and wine-processed RS aqueous extracts. There were significant differences in some pharmacokinetic parameters of most analytes between crude and wine-processed RS. This suggested that wine-processing exerted effects absorption of most flavonoids.

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Section: Analytessupporting

confidence: 86%

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Cui

Qian

Huang

et al. 2014

Biomedical Chromatography

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“…These include high-performance liquid chromatography (HPLC) and liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods for the determination of alkaloids (Tan et al, 2007;Kim et al, 2007), flavonoids Zuo et al, 2003;Li et al, 2006;Kim et al, 2006) and anthraquinones Deng et al, 2008) in plasma. Only one study using the HPLC method to simultaneously determine alkaloids, flavonoids and anthraquinones in plasma has been published, but in this study, only three components (baicalin, rhein and berberine) were analyzed (Xu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple active components from Xiexin decoction in rat plasma by LC‐ESI‐MS/MS: application in pharmacokinetics

Zan¹,

Shi²,

Wang³

et al. 2010

Biomedical Chromatography

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A sensitive and specific liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method was developed and validated to simultaneously quantify 11 active compounds (coptisine, jatrorrhizine, berberine, palmatine, baicalin, baicalein, wogonoside, wogonin, rhein, emodin and aloeemodin) from Xiexin decoction (XXD) in rat plasma. Plasma samples extracted by a single-step protein precipitation procedure were separated using the gradient mode on a Dikma ODS-C₁₈ column. Selected reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Calibration curves offered satisfactory linearity (r > 0.995) at linear range of 0.47-60 ng/mL for coptisine, jatrorrhizine, berberine and palmatine, 15-1930 ng/mL for baicalin, 20-2560 ng/mL for baicalein, 14-1790 ng/mL for wogonoside, 0.57-72.8 ng/mL for wogonin, 10-1280 ng/mL for rhein, 0.6-76.8 ng/mL for emodin and 3.0-384 ng/mL for aloeemodin. The intra- and interday precisions were less than 10.2% in terms of relative standard deviation (RSD), and the accuracies were within ±10.84% in terms of relative error (RE). It was successfully applied to the evaluation of pharmacokinetics after single oral doses of XXD were administered to rats.

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“…Bioanalysis of wogonin is essential for the evaluation of all pharmaceutical preparations intended for clinical use. Most of the previously described methods for the bioanalysis of wogonin were performed using devices such as LC-MS/MS (7)(8)(9) or HPLC/UV (10,11). In contrast, the compatibility of standard samples with test samples was not suffi ciently considered in these reports, which is against strict recommendations of the FDA guidelines for the development of bioanalytical methods.…”

Section: Compatibility Of Standard and Test Samplesmentioning

confidence: 99%

“…This can further explain the high recovery of all concentrations of wogonin from group 1 and potentiates our hypothesis of such samples being incompatible with the test samples. Analysis of previous methods of wogonin quantitation in plasma showed that changing the plasma matrix with organic solvents was widely conducted during standard sample preparations (7,8,10,(16)(17)(18)(19)21). It was performed through direct spiking of the working standard stock solutions of wogonin (with organic solvents) in ratios ranging between 10-50 % of the blank plasma volume while ignoring the consequences for drug solubility and sample integrity and hence their validity.…”

Section: Wogonin Protein Binding and Plasma Distributionmentioning

confidence: 99%

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Salmani

Jacob

et al. 2017

Acta Pharmaceutica

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In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experiencein vivowhile pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

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Liquid chromatography with tandem mass spectrometry for the simultaneous determination of baicalein, baicalin, oroxylin A and wogonin in rat plasma

Cited by 64 publications

References 30 publications

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Simultaneous quantification of multiple active components from Xiexin decoction in rat plasma by LC‐ESI‐MS/MS: application in pharmacokinetics

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

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