Liquid chromatography tandem mass spectrometry determination of maduramycin residues in the tissues of broiler chickens

Abstract

International audienceMaduramycin is a polyether ionophoric coccidiostat used to prevent coccidiosis in poultry at a prescribed concentration during a certain time interval. Due to a public health concern about the presence of coccidiostat residues in poultry, the aim of the present study was to determine the level of maduramycin residues in the tissues of broiler chickens fed commercially-produced feed containing 5 mg.kg-1 of maduramycin in complete feed throughout the 5 days of the withdrawal period (WP). The residues were investigated by liquid chromatography (LC) coupled with electrospray ionisation (ESI) tandem mass spectrometry (MS/MS) with triple quadrupole. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 0.3 μg.kg-1 and 0.8 μg.kg-1, respectively. The average recovery based on the matrix-fortified calibrations for chicken tissues was 90 %. Maduramycin was found to be rapidly distributed in all tissues. The highest concentrations of maduramycin residues were found in the heart followed by the skin, liver, gizzard, kidneys, and finally, the muscle (thigh and breast). On day 5 of the WP, the residue concentrations of maduramycin did not decline below the LOQ of the method. Our results emphasize the need to establish the maximum residue limit (MRL) for maduramycin to control the levels of its residues in edible tissues from chickens before slaughter

Development and Validation of an Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry Method to Determine Maduramicin in Crayfish (Procambarus clarkii) and Evaluate Food Safety

“…Thus, crayfish hepatopancreas tissue containing MAD likely poses a greater risk to consumers than contaminated abdominal muscle, and this information should be made known to the general public. The residue characteristics of MAD in the edible tissues of crayfish are similar to those in broiler chickens, which have the highest concentrations of MAD in the heart (285.8 ng•g −1 ) followed by those in the liver (146.8 ng•g −1 ) after withdrawal on day 0 [30]. In addition, according to Chang et al, also on day 0 after withdrawal, MAD residues in liver and breast are 627.0 ± 9.6 ng•g −1 and 52.9 ± 5.4 ng•g −1 , respectively [32].…”

“…In addition, according to Chang et al, also on day 0 after withdrawal, MAD residues in liver and breast are 627.0 ± 9.6 ng•g −1 and 52.9 ± 5.4 ng•g −1 , respectively [32]. Both studies show rapid absorption and elimination rates of MAD in chickens [30,32]. These results further indicate the proposed UPLC-MS/MS method successfully determined the presence and amounts of MAD in edible crayfish tissues.…”

“…Considering the residue and potential risk of MAD to humans and environment, numerous methods for the analytical determination of MAD and other ionophores in biological matrices (e.g., feed (LC-MS/MS), eggs (HILIC-MS/MS and electrochemical immunosensor), liver (LC-MS/MS), human plasma (LC-MS/MS), poultry tissue (LC-MS/MS)) or environmental matrices (e.g., surface water and sediment (HPLC-MS, LC-MS/MS)) have been widely reported in the literatures [2,11,[30][31][32][33][34][35]. To our knowledge, However, a method is not currently available to reliably detect MAD in crayfish.…”

Comparison of Four Microbiological Inhibition Tests for the Screening of Antimicrobial Residues in the Tissues of Food-Producing Animals

“…calidolactis to the ionophoric coccidiostats under in vitro conditions (Kožárová and Máté, 2000), and also the positive responses on the STAR test plates seeded with B. stearothermophilus var. calidolactis and with the Premi ® Test and the Total Antibiotics after the screening of tissue samples of experimental broiler chickens receiving in-feed coccidiostats (Kožárová et al, 2002(Kožárová et al, , 2008(Kožárová et al, , 2011a(Kožárová et al, , 2011bTkáčiková et al, 2010Tkáčiková et al, , 2012.…”

Comprehensive Screening of Salinomycin in Feed and Its Residues in Poultry Tissues Using Microbial Inhibition Tests Coupled to Enzyme-Linked Immunosorbent Assay (ELISA)

“…These methods are based on the detection of residues of inhibitory substances in a wide range of matrices, while the principle is the inhibition of the growth of the test organism by the action of the antibiotic present in the examined sample. Since in the case of screening it is only a matter of confirming or refuting the presence of inhibitory substances in the sample and thus it is a qualitative or semi-quantitative determination, to confirm the result it is necessary to use confirmatory methods based either on the chromatographic (LC-MS/MS) [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ] or immunological principles (enzyme-linked immunosorbent assay—ELISA) [ 10 , 35 , 36 ].…”