Comparison of Four Microbiological Inhibition Tests for the Screening of Antimicrobial Residues in the Tissues of Food-Producing Animals

Gondová, Z.; Kožárová, I.; Poláková, Zuzana; Maďarová, Michaela

doi:10.4081/ijas.2014.3521

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…This protocol applies to meat (beef, pork, and poultry), fish, shrimps, eggs, liver, kidney, urine, and feed for the residues of β -lactams, cephalosporins, macrolides, tetracyclines, sulphonamides, aminoglycosides, quinolones, amphenicols, and polypeptides. However, in a 2014 note, Gondová et al [ 96 ] using 142 slaughterhouse tissue samples stated that the STAR method and Total Antibiotics test (a EuroClone S.p.A. kit containing test tubes with Bacillus stearothermophilus var. calidolactis ) yielded better results identifying positive samples against PremiTest and NAT tests.…”

Section: Analytical Methods For Tetracyclines Determination and Rementioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

243

128

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Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of antibiotics to assess correct usage and dosage of tetracyclines in food and feedstuffs and possible residues in pertinent environmental samples. The tetracyclines are still considered a clinically relevant group of antibiotics, though dissemination of tolerance and resistance determinants have limited their use. This review focuses on four different aspects: (i) tetracyclines, usage, dosages, and regulatory issues that govern their food-related application, with particular attention to the prohibitions and restrictions that several countries have enforced in recent years by agencies from both the United States and the European Union, (ii) analytical methods for tetracyclines, determination, and residues thereof in feedstuffs and related matrices with an emphasis on the most relevant and novel techniques, including both screening and confirmatory methods, (iii) tetracycline resistance and tetracycline-resistant bacteria in feedstuff, and (iv) environmental and health risks accompanying the use of tetracyclines in animal nutrition. In the last two cases, we discuss the more relevant undesirable effects that tetracyclines exert over bacterial communities and nontarget species including unwanted effects in farmers.

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Section: Analytical Methods For Tetracyclines Determination and Rementioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

243

128

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“…(Cháfer-Pericás et al, 2010;Pikkemaat, 2009). Few methodologies based on microbial growth inhibition have been developed, they were applied to food control in livestock production (Gondová et al, 2014;Pikkemaat et al, 2008), in seafood like shrimps (Dang et al, 2010) and in trout (Barker, 1994). The use of biota biofluids (such as mussel hemolymph) instead of organism's tissues (like mussels soft tissue) extract also allows simplifying the extraction protocol and reducing the potential loss of antibiotics during the extraction procedure.…”

Section: Introductionmentioning

confidence: 99%

Combining an effect-based methodology with chemical analysis for antibiotics determination in wastewater and receiving freshwater and marine environment

Serra-Compte

Pikkemaat

Elferink

et al. 2021

Environmental Pollution

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“…Several tests, including microbiological, chromatography‐based, immunological, physicochemical and biological sensing systems, have been developed for the detection of antimicrobial drug residues in animal products (Chen et al., 2017; Gondová et al., 2014; Ramatla et al., 2017). Among them, microbial assays are used largely for monitoring of antimicrobial drug residues because these methods are inexpensive and have detection limits close to the MRL for beta‐lactams, fluoroquinolones, tetracyclines and sulfonamides.…”

Section: Introductionmentioning

confidence: 99%

A microbial inhibition assay in microplates using Bacillus licheniformis for detection of enrofloxacin and sulfamethazine in chicken spiked kidney, liver and muscle tissues

Mirzaie,

Jamiri,

Javanmard Dakheli

et al. 2023

Veterinary Medicine & Sci

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BackgroundThe presence of antimicrobial drugs residues in animal products at levels higher than the maximum residue level (MRL) may have adverse effects on consumer health such as allergic reactions and resistance development. Therefore, it is necessary to monitor animal products for the presence of antimicrobial residues.ObjectivesThe aim of this study was to evaluate the detection limit of microbial inhibition assay (MIA) in microplate by using of Bacillus licheniformis as indicator microorganism for two antibiotics, enrofloxacin (ENR) and sulfamethazine (SMT), in broiler chicken's kidney, liver and muscle tissue samples.MethodsSpiked tissues samples for the two antibiotics were analysed separately by this method. The results of the assay were evaluated by the determination of the absorbance after mean 3.47 h of incubation at 45°C.ResultsResults showed that the detection limits of MIA for ENR and SMT in kidney (124.03 and 23.21 μg/kg, respectively) and liver (90.02 and 62.03 μg/kg, respectively) as well as SMT in muscle (46.95 μg/kg) were lower than EU (European Union) – MRL, whereas the detection limit for ENR in muscle was slightly higher than MRL (136.3 μg/kg compared to 100 μg/kg MRL). Furthermore, the MIA in the current study was found to be more sensitive to SMT than ENR (92% and 88% sensitivity rate, respectively). No false‐positive was observed in the assay.ConclusionsBased on the results, the MIA investigated in this study had the potential to detect ENR and SMT residues in broiler chicken kidney, liver and muscle tissues at levels below or close to EU – MRL but offered lower capability for the detection of ENR compared with SMT in kidney and muscle tissue samples.

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Comparison of Four Microbiological Inhibition Tests for the Screening of Antimicrobial Residues in the Tissues of Food-Producing Animals

Cited by 14 publications

References 17 publications

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Combining an effect-based methodology with chemical analysis for antibiotics determination in wastewater and receiving freshwater and marine environment

A microbial inhibition assay in microplates using Bacillus licheniformis for detection of enrofloxacin and sulfamethazine in chicken spiked kidney, liver and muscle tissues

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