Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma

Gowda, K. Veeran; Mandal, Uttam Kumar; Selvan, P. Senthamil; Solomon, Wesley; Ghosh, Animesh; Sarkar, Amlan Kanti; Agarwal, Sangita; Rao, Tai; Pal, Tapan Kumar

doi:10.1016/j.jchromb.2007.07.047

Cited by 53 publications

(28 citation statements)

References 30 publications

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“…Literature survey revealed that various spectrophotometric and chromatographic methods have been reported for estimation of MET [3][4][5][6][7][8][9][10][11] and OLM [12][13][14][15][16][17][18] individually or in combination with other drugs. To the best of our knowledge, there is no HPTLC method reported for analysis of metoprolol succinate and olmesartan medoxomil in pharmaceutical formulations.…”

Section: Metoprolol Succinate (Met) Is Chemically 1-[4-(2-methoxyethymentioning

confidence: 99%

Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

Baldania

Parmar

Bhatt

et al. 2012

ISRN Analytical Chemistry

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A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method is described for simultaneous analysis of metoprolol succinate and olmesartan medoxomil in pharmaceutical formulations. The separations were achieved on prepared TLC plates precoated with silica gel G 60 F 254 and the plates were developed with methanol-ethyl acetatetoluene-glacial acetic acid 2.5 : 3 : 4.5 : 0.3%v/v/vas mobile phase. Detection and evaluation of chromatograms were performed densitometrically at 224 nm. The retention factor of metoprolol succinate and olmesartan medoxomil was 0.25 and 0.70, respectively. The linear range was 1000-2250 ηg per spot for metoprolol succinate and 800-1800 ηg per spot for olmesartan medoxomil and the correlation coefficients (r) were 0.993 and 0.999, respectively. The method was validated for linearity and range, specificity, precision, accuracy, and robustness, and the results were found to be within the acceptance criteria. The method was successfully applied to the analysis of drugs in their capsule dosage form.

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Section: Metoprolol Succinate (Met) Is Chemically 1-[4-(2-methoxyethymentioning

confidence: 99%

Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

Baldania

Parmar

Bhatt

et al. 2012

ISRN Analytical Chemistry

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“…These methods include liquid chromatography coupled with mass spectrometry (LC-MS/MS) [7][8][9][10][11][12][13][14][15][16][17], liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) [18,19], gas chromatography coupled with mass spectrometry (GC-MS) [3,[20][21][22], high performance liquid chromatography (HPLC) [23,24], high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) [25,26], high performance liquid chromatography with fluorescence detection (HPLC-FLD) [2,27,28], high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) [29], reversed-phase ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) [30].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

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Arterial hypertension is a complex disease with many serious complications, representing a leading cause of mortality. Selective beta-blockers such as metoprolol and bisoprolol are frequently used in the management of hypertension. Numerous analytical methods have been developed for the determination of these substances in biological fluids, such as liquid chromatography coupled with mass spectrometry, gas chromatography coupled with mass spectrometry, high performance liquid chromatography. Due to the complex composition of biological fluids a biological sample pre-treatment before the use of the method for quantitative determination is required in order to remove proteins and potential interferences. The most commonly used methods for processing biological samples containing metoprolol and bisoprolol were identified through a thorough literature search using PubMed, ScienceDirect, and Willey Journals databases. Articles published between years 2005-2015 were reviewed. Protein precipitation, liquid-liquid extraction and solid phase extraction are the main techniques for the extraction of these drugs from plasma, serum, whole blood and urine samples. In addition, numerous other techniques have been developed for the preparation of biological samples, such as dispersive liquid-liquid microextraction, carrier-mediated liquid phase microextraction, hollow fiber-protected liquid phase microextraction, on-line molecularly imprinted solid phase extraction. The analysis of metoprolol and bisoprolol in human plasma, urine and other biological fluids provides important information in clinical and toxicological trials, thus requiring the application of appropriate extraction techniques for the detection of these antihypertensive substances at nanogram and picogram levels.

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“…A combination dosage form of HCT with the β1-selective (cardio selective) -β-blocker Metoprolol tartarate (MET) (Smilide, 1983), is more effective than either drug alone in the management of hypertension and is effective in patients unresponsive to immunotherapy with either agent (Papademetriou et al, 2006;Hainer, 2007). For the determination of MET in human plasma, several methods based on high performance liquid chromatography (HPLC) with UV (Aqil et al, 2007) and fluorescence (Zhaing, 2006) detection, gas chromatography-mass spectrometry (Yilmaz, 2009) and LC-MS/MS (Gowda et al, 2007;Gao et al, 2010) have been reported. Similarly, a method for the determination of HCT in human plasma has been developed based on HPLC with UV or diode array detection (Zendelovska, 2004;Erk, 2003) and LC-MS/MS (Ramakrishna et al, 2005;Liu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical method development and validation for determination of metoprolol tartarate and hydrochlorothiazide using HPTLC in human plasma

Rote

Sonavane

2013

Braz. J. Pharm. Sci.

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A simple, sensitive, rapid and economic chromatographic method has been developed for determination of metoprolol tartarate and hydrochlorothiazide in human plasma using paracetamol as an internal standard. The analytical technique used for method development was high-performance thin-layer chromatography. HPTLC Camag with precoated silica gel Plate 60F 254 (20 cm×10 cm) at 250 µm thicknesses (E. Merck, Darmstadt, Germany) was used as the stationary phase. The mobile phase used consisted of chloroform: methanol: ammonia (9:1:0.5v/v/v). Densitometric analysis was carried out at a wavelength of 239 nm.The rf values for hydrochlorothiazide, paracetamol and metoprolol tartarate were 0.13±0.04, 0.28±0.05, 0.48±0.04, respectively. Plasma samples were extracted by protein precipitation with methanol.

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Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma

Cited by 53 publications

References 30 publications

Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Bioanalytical method development and validation for determination of metoprolol tartarate and hydrochlorothiazide using HPTLC in human plasma

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