“…The method was validated for system suitability, autosampler carryover, specificity and screening of the biological matrix, sensitivity, matrix effect, linearity, precision and accuracy, recovery of analyte and internal standard, ruggedness on precision accuracy and linearity (Maring et al, 2005;Rote et al, 2013;Phatak, Vaidya, Phatak, 2014), reinjection reproducibility and stability on day zero, long batch, LT at -28 °C and LT at -80 °C.…”