Bioanalytical method development and validation for determination of metoprolol tartarate and hydrochlorothiazide using HPTLC in human plasma

Rote, Ambadas R.; Sonavane, Poonam R.

doi:10.1590/s1984-82502013000400025

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…The method was validated for system suitability, autosampler carryover, specificity and screening of the biological matrix, sensitivity, matrix effect, linearity, precision and accuracy, recovery of analyte and internal standard, ruggedness on precision accuracy and linearity (Maring et al, 2005;Rote et al, 2013;Phatak, Vaidya, Phatak, 2014), reinjection reproducibility and stability on day zero, long batch, LT at -28 °C and LT at -80 °C.…”

Section: Methods Validationmentioning

confidence: 99%

HPLC method development and validation for the estimation of Axitinib in rabbit plasma

Suneetha

Donepudi²

2017

Braz. J. Pharm. Sci.

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A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinib (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinib is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for axitinib and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for axitinib with a correlation coefficient of r 2 0.999.

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Section: Methods Validationmentioning

confidence: 99%

HPLC method development and validation for the estimation of Axitinib in rabbit plasma

Suneetha

Donepudi²

2017

Braz. J. Pharm. Sci.

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“…Rote et al has discussed double drug combinations of HCTZ and metoprolol tartrate (METO) using Human Plasma where PARA is an internal standard and PP as a bioanalytical extraction technique. The linearity range was found to be 2-12 ng mL À1 and 20-120 ng mL À1 for HCTZ and METO respectively [63]. Table 3 depicted the bioanalytical methods represented for the quantification of HCTZ and its combinations using HPTLC.…”

Section: High-performance Thin Layer Chromatography (Hptlc)mentioning

confidence: 99%

Bioanalytical methods and extraction techniques for the quantification of hydrochlorothiazide and its pharmaceutical combinations: A comprehensive review

Chakraborty,

Jayaseelan,

Rathinam

et al. 2024

AChrom

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Hydrochlorothiazide has been utilized clinically for the past half-century, which is popularly known as a “water pill” as it produces increased urine output. The advancement of bioanalytical methods brought a dynamic field with exciting opportunities for future research. The current review emphasis the bioanalytical methods employed for the quantitative estimation of Hydrochlorothiazide as monotherapy and its popularly used combinational medications available from 1956 to till date. A fixed dose of 25 mg of hydrochlorothiazide with 43 combinational medications is currently available in the market and these combinations are widely employed in the treatment of hypertensive people; those whose blood pressure does not respond effectively to monotherapy of hydrochlorothiazide and also for the treatment of edema (excess fluid in the body) caused by illness such as heart failure, liver problems, and renal disease. It has been convincingly demonstrated that the combination of any two antihypertensive medications belonging to different groups of the same category, significantly lowers blood pressure, in comparison with the effect produced by increasing the dose of a single medicament. Among the various analytical techniques employed for the estimation of Hydrochlorothiazide, the review portrays that hyphenated technique, in specific liquid chromatography coupled with mass spectroscopy was widely employed. The validation parameters namely linearity, LOD, LOQ for individual drug and their combinations, were successfully calibrated. The effectiveness of analytical approaches was evaluated and enhanced for chemical factors. The involvement of green chemistry in the optimized methods for the evaluation of Hydrochlorothiazide for the future development, are suggested.

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“…Spectroscopic [8][9][10], High Performance Liquid Chromatography (HPLC) [11][12][13], Thin Layer Chromatography (TLC) [14], Electrochemical [15][16][17] and mass spectrometry [18] methods for estimation of MOX alone or in combination with other drugs in pharmaceutical preparation have been reported. Hydrochlorothiazide alone or in combination with other drugs is estimated by HPLC [19][20][21][22][23][24][25][26][27][28][29], TLC-densitometry [30][31][32][33][34][35][36], capillary electrophoresis [37][38][39], electrochemical [40][41][42][43] and spectroscopic methods [44][45][46][47][48][49][50][51][52][53]. Simultaneous estimation of MOX and HCT has been reported by RP-HPLC [54,…”

Section: Moexipril Hydrochloride (Mox) Is Chemically (3s)-2-[(2s)-2-{mentioning

confidence: 99%

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

Kelani¹,

Shalaby²,

El-Maamly³

et al. 2014

Pharm Anal Acta

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In this study determination of Moexipril hydrochloride (MOX) and hydrochlorothiazide (HCT) were conducted by application of Spectrophotometric and Chemometric methods. Five different accurate, sensitive and reproducible methods were applied for the simultaneous determination of (MOX) and (HCT) in their bulk powder and pharmaceutical dosage form. The first method is the new absorbance subtraction (AS) method. The second method is the new amplitude modulation (AM) method. The third method is the new extended ratio subtraction (ERS) method coupled to ratio subtraction (RS) method. The fourth and fifth methods are multivariate calibration which includes Principal Component Regression (PCR) and Partial Least Squares (PLS). The suggested procedures were checked using laboratory prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The validity of the proposed methods was further assessed by applying the standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with a reported method.

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Bioanalytical method development and validation for determination of metoprolol tartarate and hydrochlorothiazide using HPTLC in human plasma

Cited by 8 publications

References 12 publications

HPLC method development and validation for the estimation of Axitinib in rabbit plasma

HPLC method development and validation for the estimation of Axitinib in rabbit plasma

Bioanalytical methods and extraction techniques for the quantification of hydrochlorothiazide and its pharmaceutical combinations: A comprehensive review

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

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