Liquid chromatography–mass spectrometry method for the quantification of tamoxifen and its metabolite 4-hydroxytamoxifen in rat plasma: Application to interaction study with biochanin A (an isoflavone)

Simultaneous determination of erlotinib and tamoxifen in rat plasma using UPLC–MS/MS: Application to pharmacokinetic interaction studies

“…3. Using this method, the achieved LLOQ values of both drugs were lower than those of previously reported LC-MS/MS methods for ERL [18][19][20][21][22] and TAM [26,[29][30][31][32][33][34][35][36]. These low values of LLOQ allowed the successful application of this method in the trace analysis of the two drugs in clinical studies.…”

“…With the exception of ERL which was previously determined by our research team in rat plasma samples, in combinations with gefitinib, dexamethasone, prednisolone, and ondansetron [23], the current method provided the lowest LLOQ (0.2 ng/mL) described so far for the analysis of TAM. Compared with previously published methods for the determination of ERL [18][19][20][21][22] or TAM [26,[29][30][31][32][33][34][35][36], this method was of a remarkable sensitivity. This is extremely important for trace analysis of the studied drugs and for accurate determination of terminal phase elimination during PK studies.…”

“…This is extremely important for trace analysis of the studied drugs and for accurate determination of terminal phase elimination during PK studies. Moreover, some of these previous reports utilized less advanced sample preparation techniques like protein precipitation [18-20, 29, 30, 33, 35] or liquid-liquid extraction [31,34], had longer analysis time [20,22,[29][30][31][32][33][34][35], or even utilized larger sample volume for analysis [20,22,31,[33][34][35][36]. Our developed method depends on the analysis of only very small sample volume (50 µL) which is extremely beneficial in cases where only limited amounts of samples are available.…”

“…They include HPLC-UV [15,16], HPLC-DAD [17], HPLC-MS/MS [18][19][20][21], and UPLC-MS/MS [22,23]. Also, TAM has been analyzed in biological matrices using numerous liquid chromatographic techniques, namely HPLCfluorimetry [24][25][26], HPLC-DAD [27], HPLC-UV [28], HPLC-MS/MS [26,[29][30][31][32], and UPLC-MS/MS [33][34][35][36]. Since UPLC-MS/MS is nowadays the technique of choice in bioanalysis, regarding selectivity and sensitivity, it was successfully applied in studying PK interaction studies of ERL [23] and TAM [31,36].…”

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

“…The other components were tentatively identified according to their MS fragmentation pattern, and also by studying their UV spectral characteristics and reported literatures. Accordingly, the peaks were ascribed to cytidine (1) [27,28], uracil (2) [29,30], guanosine (4) [31,32], adenine nucleoside (6) [17,18], syringoside (7) [33,34] (10) [35], isomucronulatol-7,2′-di-O-glucoside (11) [36], biochanin-7-glucoside (12) [37,38], 9,10-dimethoxypterocarpan-3-O-xylosylglucoside (15) [39], 9,10-dimethoxypterocarpan-3-O-β-D-glucoside (16) [39], isomucronulatol-7-O-glucoside (17) [36,40], astragaloside VI (1′) [26,39,41], astragaloside VII (2′) [26,39,41], and astragaloside II (4′) [39,[41][42][43]. However, they still need to be further confirmed with the reference compounds and structural analysis by nuclear magnetic resonance (NMR) method.…”