Simultaneous determination of erlotinib and tamoxifen in rat plasma using UPLC–MS/MS: Application to pharmacokinetic interaction studies

Maher, Hadir M.; Alzoman, Nourah Z.; Shehata, Shereen M.

doi:10.1016/j.jchromb.2016.05.033

Cited by 14 publications

(8 citation statements)

References 38 publications

(87 reference statements)

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“…On the other hand, an increase in the peak concentration ( C max ) of TAM of (46%), with no significant increase in the AUC (10%), was observed with TAM combined with ERL compared with its single administration. This result could be related to the direct CYP3A inhibition effect of ERL leading to increased levels in plasma (Maher, Alzoman, & Shehata, ). Another study studied the possibility of PK interactions of selected TKIs (gefitinib GEF and erlotinib ERL) taken in combination with corticosteroids (dexamethasone and prednisolone) and the antiemetic ondansetron in rat plasma samples (Maher, Alzoman, & Shehata, ).…”

Section: Introductionmentioning

confidence: 99%

UPLC–MS/MS study of the effect of dandelion root extract on the plasma levels of the selected irreversible tyrosine kinase inhibitors dasatinib, imatinib and nilotinib in rats: Potential risk of pharmacokinetic interactions

Alzoman

Maher

Shehata

et al. 2019

Biomedical Chromatography

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Tyrosine kinase inhibitor treatments for chronic myeloid leukaemia based on nilotinib (NIL), dasatinib (DAS) and imatinib (IMA) have improved patient quality of life and have turned chronic myeloid leukemia from a fatal disease into a chronic disease.Dandelion is a rich source of phenolic compounds with strong biological properties, and the effects of using this plant in the treatment of different illnesses can be linked to the presence of various polyphenols found in the different parts of the plant. Thus, dandelion can potentially be used as a nutraceutical (dietary antioxidant) to prevent different disorders associated with oxidative stress, i.e. cardiovascular disorders, cancer and inflammatory processes. Mutual interference between a drug and a food constituent may result in altered pharmacokinetics of the drug and undesired or even dangerous clinical situations. In the present study, a bioanalytical ultra performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) method was developed and validated for the quantification of DAS, IMA and NIL in rat plasma.Sample preparation was carried out using solid-phase extraction with C 18 cartridges with a good extraction recovery of ≥94.37% for the three drugs. The method was fully validated as per the US Food and Drug Administration guidelines.

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Section: Introductionmentioning

confidence: 99%

UPLC–MS/MS study of the effect of dandelion root extract on the plasma levels of the selected irreversible tyrosine kinase inhibitors dasatinib, imatinib and nilotinib in rats: Potential risk of pharmacokinetic interactions

Alzoman

Maher

Shehata

et al. 2019

Biomedical Chromatography

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“…Thus, this method (PPT/SPE) was applied in this work. Based on our previous work for the simultaneous analysis of erlotinib and TAM in rat plasma 29 , Strata TM −X-C 33 µm strong cation cartridges (200 mg, 3 mL) (Phenomenex, Torrance, USA) was found optimum since it provided better and consistent extraction recoveries of the analytes, compared with other SPE cartridges which had been tried (octadecyl C 18, octyl C 8, ethyl C 2, and cyanopropyl CN (200 mg, 3 mL) (Spe-ed SPE car-tridges, Applied Separations, Allentown, PA). Being weakly basic compounds (The pKa values of DSV are 8.2 (pK 1 ) and 9.2 (pK 2 ), pka of TAM is 8.76, pka values of TOH are 8.66, 9.45) 41 , cation mixed mode cartridges, applying both reversed phase and ionic interactions for analyte retention, produced efficient recovery of the three analytes.…”

Section: Resultsmentioning

confidence: 99%

“…Being weakly basic compounds (The pKa values of DSV are 8.2 (pK 1 ) and 9.2 (pK 2 ), pka of TAM is 8.76, pka values of TOH are 8.66, 9.45) 41 , cation mixed mode cartridges, applying both reversed phase and ionic interactions for analyte retention, produced efficient recovery of the three analytes. Moreover, we previously reported that, following experimental trials, the optimum operating procedure for TAM extraction was as follows 29 ; 50 µL plasma samples were spiked with the analytes and the IS, treated with 150 µL 2% formic acid, and then completed to 1-mL using acetonitrile for PPT. Following centrifugation, further purification was performed using Strata TM −X-C 33 µm SPE cartridges which had been preconditioned with 3 mL water followed by 3 mL 2% formic acid.…”

Section: Resultsmentioning

confidence: 99%

“…Accordingly, they could affect the PK of co-administered drugs. Our research team has previously studied the PK interaction between TAM and the anticancer drug erlotinib (ERL) 29 where an increase in TAM C max with no significant increase in AUC, along with a decrease in AUC of ERL were recorded.…”

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“…Drug plasma concentrations of TAM, the active metabolite TOH, and DSV were measured using newly developed UPLC-MS/MS. This technique was previously reported for the bioanalysis of either TAM/TOH [29][30][31][32][33] or DSV 34,35 . The proposed method has many advantages over the previously reported methods; it provided lower LLOQ for the determination of TAM/TOH 21,30,31,33 .…”

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Development and validation of UPLC-MS/MS method for studying the pharmacokinetic interaction of dasabuvir and tamoxifen, 4-hydroxytamoxifen in Wistar rats

Almomen

Maher

Alzoman

et al. 2020

Sci Rep

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Hepatitis c virus (HcV) is the main cause of chronic hepatitis and probably liver cirrhosis. Dasabuvir(DSV) is a direct-acting antiviral agent with efficiency in managing HCV. The anti-viral activity of the anti-estrogen drug tamoxifen (TAM) suggested the synergistic effect of DSV and TAM for blocking the replication of HCV. However, being substrates and inhibitors of efflux transporters (TAM inhibits P-gp, DSV inhibits p-gp and BcRp), there is a possibility for a pharmacokinetic (pK) drug-drug interaction. in this work, a new UpLc-MS/MS method was developed and validated for the simultaneous determination of TAM, its active metabolite 4-hydroxy tamoxifen (TOH), and DSV in rat plasma. The method was applied to investigate the pK interaction between DSV and tAM/toH following the coadministration of DSV and TAM to Wistar rats. Chromatographic analysis was performed on Waters BeH tM C18 column using a mobile phase of acetonitrile/water containing 0.1% formic acid (80: 20, v/v). The method allowed the determination of concentration ranges 20-1000, 0.1-500, 0.5-500 ng/mL for DSV, tAM, and toH, respectively. Unexpectedly, results revealed the absence of pK interactions between DSV and tAM/toH, compared with their single administration, suggesting the safety of coadministering DSV/tAM as an anti-viral combination without the need of dosage adjustment.www.nature.com/scientificreports www.nature.com/scientificreports/ Matrix effect. For matrix effect, calculations were based on comparing the peak responses from the four QC samples, used for evaluating the "extraction recovery", spiked post-extraction with those of standard acetonitrile drug solutions prepared of the same nominal concentrations. Scientific RepoRtS |(2020) 10:3521 | https://doi.

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Analysis of tamoxifen and its main metabolites in plasma samples of breast cancer survivor female athletes: Multivariate and chemometric optimization

Sadeghcheh

Tehrani

Faraji

et al. 2022

J of Separation Science

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A sensitive method based on liquid chromatography combined with a diode array detector was developed and validated to simultaneously determine tamoxifen, and its active metabolites N‐desmethyltamoxifen, 4‐hydroxytamoxifen, and endoxifen in human plasma samples. The green and sustainable vortex‐assisted dispersive liquid‐phase microextraction technique based on the natural hydrophobic deep eutectic solvent was used for the extraction and preconcentration of the analytes. Chemometrics and multivariate analysis were used to optimize the independent variables including the type and volume of deep eutectic solvent, extraction time, and ionic strength. Under optimal conditions, calibration curves were linear in a suitable range with the lower limits of quantification (0.8–10.0 μg/L), which covered the relevant concentrations of the analytes in plasma samples for a clinical study. Intra‐ and interday precision evaluated at three concentrations for the analytes were lower than 8.2 and 12.1%, respectively. Accuracy was in the range of 94.9–104.7%. The applicability of the developed method on human plasma samples illustrated the range 45.1–72.8, 98.4–128.3, 0.9–1.2, and 2.7–6.1 μg/L for tamoxifen, N‐desmethyltamoxifen, 4‐hydroxytamoxifen, and endoxifen, respectively. The validated method can be effective for the pharmacokinetics, pharmacodynamics, and therapeutic drug monitoring studies of tamoxifen and its main metabolites in biological fluids.

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Simultaneous determination of erlotinib and tamoxifen in rat plasma using UPLC–MS/MS: Application to pharmacokinetic interaction studies

Cited by 14 publications

References 38 publications

UPLC–MS/MS study of the effect of dandelion root extract on the plasma levels of the selected irreversible tyrosine kinase inhibitors dasatinib, imatinib and nilotinib in rats: Potential risk of pharmacokinetic interactions

UPLC–MS/MS study of the effect of dandelion root extract on the plasma levels of the selected irreversible tyrosine kinase inhibitors dasatinib, imatinib and nilotinib in rats: Potential risk of pharmacokinetic interactions

Development and validation of UPLC-MS/MS method for studying the pharmacokinetic interaction of dasabuvir and tamoxifen, 4-hydroxytamoxifen in Wistar rats

Analysis of tamoxifen and its main metabolites in plasma samples of breast cancer survivor female athletes: Multivariate and chemometric optimization

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