Liquid Chromatography in Pharmaceutical Analysis XI: Determination of Muscle Relaxant-Analgesic Mixtures Using Reversed-Phase and Ion-Pair Techniques

Stewart, James T.; Honigberg, I. L.; Coldren, J.W.

doi:10.1002/jps.2600680112

Cited by 23 publications

(3 citation statements)

References 8 publications

(7 reference statements)

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“…The United States Pharmacopoeia (USP) (3) recommends a spectrophotometric method for the determination of methocarbamol raw material and highperformance liquid chromatography (HPLC) for its tablets. Several analytical methods have been reported for the determination of methocarbamol in tablets and human plasma such as spectrofluorimetry (4), spectrophotometry (5)(6)(7)(8)(9), electrochemical method (10), H 1 -NMR spectroscopy (11), GC (12)(13)(14), HPLC (15)(16)(17)(18)(19)(20)(21)(22)(23)(24) and supercritical fluid chromatography (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

2012

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A new simple, rapid and sensitive reversed‐phase liquid chromatographic method was developed and validated for the simultaneous determination of methocarbamol (MET) and aspirin (ASP) in their combined dosage form. The separation of these compounds was achieved within 6.0 min on a CLC Shim‐pack C8 column (250 × 4.6 mm, 5 µm particle size) using isocratic mobile phase consisting of acetonitrile and 0.02 M dihydrogenphosphate buffer (30:70, v/v) at pH = 5.0. The analysis was performed at a flow rate of 1.0 mL/min with fluorescence detection at 277/313 nm for MET and 298/410 nm for ASP using real‐time programming. The selectivity, linearity of calibration, accuracy, inter‐ and intra‐day precision and recovery were examined as parts of the method validation. The concentration–response relationship was linear over concentration ranges of 0.02‐0.20 and 0.02‐0.40 µg/mL for MET and ASP, respectively, with a limit of detection of 6 and 32 ng/mL for MET and ASP, respectively. The proposed method was successfully applied for the analysis of both MET and ASP in prepared tablets with average recoveries of 99.88 ± 0.65% for MET and 100.44 ± 0.78% for ASP. The results were favourably compared to those obtained by a reference method. Copyright © 2012 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

2012

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“…Methods used for the assay of MT in pharmaceutical and biological samples include GC18 and HPLC 19–22. In the United States Pharmacopoeia (USP) 2007, the assay of MT in dosage forms also relies on HPLC determination 23.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous spectrophotometric determination of diclofenac potassium and methocarbamol in binary mixture using chemometric techniques and artificial neural networks

Elkady

2010

Drug Testing and Analysis

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In this study, the simultaneous determination of diclofenac potassium (DP) and methocarbamol (MT) by chemometric approaches and artificial neural networks using UV spectrophotometry has been reported as a simple alternative to using separate models for each component. Three chemometric techniques-classical least-squares (CLS), principal component regression (PCR), and partial least-squares (PLS)-along with radial basis function-artificial neural network (RBF-ANN) were prepared by using the synthetic mixtures containing the two drugs in methanol. A set of synthetic mixtures of DP and MT was evaluated and the results obtained by the application of these methods were discussed and compared. In CLS, PCR, and PLS, the absorbance data matrix corresponding to the concentration data matrix was obtained by the measurements of absorbances in the range 260-310 nm in the intervals with Δλ = 0.2 nm in their zero-order spectra. Then, calibration or regression was obtained by using the absorbance data matrix and concentration data matrix for the prediction of the unknown concentrations of DP and MT in their mixtures. In RBF-ANN, the input layer consisting of 251 neurons, 9 neurons in the hidden layer, and 2 output neurons were found appropriate for the simultaneous determination of DP and MT. The accuracy and the precision of the four methods have been determined and they have been validated by analyzing synthetic mixtures containing the two drugs. The proposed methods were successfully applied to a pharmaceutical formulation containing the examined drugs.

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“…Traditional techniques for detection of methocarbamol in biological samples include thin-layer chromatography (TLC) (5), carbon-14-1abeled drug use (3), spectrophotometric determination of the formaldehyde derivative of methocarbamol (6), and HPLC with refractive index detection (7). HPLC with IN and photodiode array detection (4,(8)(9)(10)(11) has been the method of choice recently for the detection of this drug in forensic toxicology.…”

Section: Introductionmentioning

confidence: 99%

Determination of Methocarbamol in Equine Serum and Urine by High-Performance Liquid Chromatography with Ultraviolet Detection and Atmospheric Pressure Ionization-Mass Spectrometric Confirmation*

Koupai-Abyazani¹,

Esaw²,

Laviolette³

1997

Journal of Analytical Toxicology

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Urine and serum samples collected from four standard-bred mares after and oral regimen administration of methocarbamol were extracted and analyzed. The method consisted of enzyme hydrolysis followed by a one-step liquid-liquid extraction, separation on a reversed-phase (RP-18) column, and detection using an ultraviolet (UV) detector. The confirmation was carried out using a liquid chromatography-atmospheric pressure ionization-mass spectrometry (LC-API-MS) system. Maximum methocarbamol concentrations of 1498, 1734, 1547, 2322 micrograms/mL in urine and 4.9, 1.7, and 3.6 micrograms/mL in serum were observed. The peak concentrations of the drug were detected 1-4 h (urine) and 10-60 min (serum) after administration to four horses. The method validation results and drug elimination profiles for both urine and serum are presented and discussed.

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Liquid Chromatography in Pharmaceutical Analysis XI: Determination of Muscle Relaxant-Analgesic Mixtures Using Reversed-Phase and Ion-Pair Techniques

Cited by 23 publications

References 8 publications

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

Simultaneous spectrophotometric determination of diclofenac potassium and methocarbamol in binary mixture using chemometric techniques and artificial neural networks

Determination of Methocarbamol in Equine Serum and Urine by High-Performance Liquid Chromatography with Ultraviolet Detection and Atmospheric Pressure Ionization-Mass Spectrometric Confirmation*

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