Liquid chromatography assay for 5-aminolevulinic acid: Application to in vitro assessment of skin penetration via Dermaportation

Namjoshi, Sarika; Caccetta, Rima; Edwards, Jeffrey E.; Benson, Heather A. E.

doi:10.1016/j.jchromb.2006.12.040

Cited by 35 publications

(35 citation statements)

References 29 publications

(37 reference statements)

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“…Insulin, ketoprofen and vaccines [93,95,165] Increased driving force technologies Iontophoresis Transdermal delivery by a small direct current Charged molecules, peptides, proteins and indomethacin [97,98,166] Dermaportation or magnetophoresis Delivery using static or variable magnetic fields Benzoic acid, terbutaline sulfate, 5-ALA, Ala-Trp (dipeptide) and naltrexone [100][101][102][103][104] ALA: Aminolevulinic acid.…”

Section: Cavitation Technologiesmentioning

confidence: 99%

Delivery of drugs applied topically to the skin

Leite-Silva¹,

Almeida²,

Fradin

et al. 2012

Expert Review of Dermatology

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Section: Cavitation Technologiesmentioning

confidence: 99%

Delivery of drugs applied topically to the skin

Leite-Silva¹,

Almeida²,

Fradin

et al. 2012

Expert Review of Dermatology

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“…However heating at 100°C was required in their method, which can lead to the degradation of ALA. 19 Namjoshi et al developed a derivatization reaction that can be carried out at room temperature using fluorescamine, which reacts readily and instantaneously with ALA at room temperature. 15 After the reaction is complete, the residual fluorescamine is rapidly converted to nonfluorescent products. This method provides a reliable, simple, and quick alternative for ALA determination.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 1). The derivative was analyzed by high performance liquid chromatography (HPLC) as described by Namjoshi et al 15 In brief, samples and controls were dissolved in phosphate buffered saline (PBS) at pH 5.0. Fluorescamine solution (0.1%) was prepared by dissolving fluorescamine in acetone solution.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles

Shi¹,

Wang²,

Zhao³

et al. 2013

IJN

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Background: 5-Aminolevulinic acid (ALA) is a prodrug for topical photodynamic therapy. The effectiveness of topical ALA can be limited by its bioavailability. The aim of this study was to develop a novel ALA delivery approach using poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). Methods: A modified double emulsion solvent evaporation method was used to prepare ALA loaded PLGA NPs (ALA PLGA NPs). The characteristics, uptake, protoporphyrin IX fluorescence kinetics, and cytotoxicity of ALA PLGA NPs toward a human skin squamous cell carcinoma cell line were examined. Results: The mean particle size of spherical ALA PLGA NPs was 65.6 nm ± 26 nm with a polydispersity index of 0.62. The encapsulation efficiency was 65.8% ± 7.2% and ALA loading capacity was 0.62% ± 0.27%. When ALA was dispersed in PLGA NPs, it turned into an amorphous phase. ALA PLGA NPs could be taken up by squamous cell carcinoma cells and localized in the cytoplasm. The protoporphyrin IX fluorescence kinetics and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay showed that ALA PLGA NPs were more effective than free ALA of the same concentration. Conclusion: PLGA NPs provide a promising ALA delivery strategy for topical ALAphotodynamic therapy of skin squamous cell carcinoma.

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“…Fluorescamine (4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione) is a widely-used reagent for the analysis of primary amine groups, as it produces highly fluorescent pyrrolidone derivatives [13,14]. Fluorescamine is not fluorescent itself but can react with a primary amine at room temperature, resulting in a fluorophore with strong intensity in both aqueous and non-aqueous systems [15].…”

Section: Introductionmentioning

confidence: 99%

Development and Analysis of Functionalized Poly(Lactide- Co-Glycolide)Polymer for Drug Delivery

Chen¹,

Huda²,

Benson³

2017

SOJPPS

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Functionalized polymer molecules are the backbone for formulation of targeted biomolecule delivery systems. We report here an effective method for the chemical synthesis of poly (lactide-co-glycolide)-1,4 diaminobutane (PLGA-DAB) in a controlled manner and an efficient gel permeation chromatography (GPC) method for analysis of functionalized PLGA. The synthetic method is simple, reproducible and the functionalized PLGA can be conjugated with any carboxylic acid-containing ligand and ultimately used for targeted drug delivery. The developed GPC analytical method is accurate, selective, precise and easily adaptable for analysing any other functionalized polymer with an amine group. These techniques provide an important basis for formulation scientists to develop functionalized polymers for targeted nanoparticle drug delivery.

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Liquid chromatography assay for 5-aminolevulinic acid: Application to in vitro assessment of skin penetration via Dermaportation

Cited by 35 publications

References 29 publications

Delivery of drugs applied topically to the skin

Delivery of drugs applied topically to the skin

In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles

Development and Analysis of Functionalized Poly(Lactide- Co-Glycolide)Polymer for Drug Delivery

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