Liquid chromatography and radioimmunoassay method for the determination of prostaglandins E1 and E2 in rat embryo incubates

Hotter, Georgina; Roselló‐Catafau, Joan; Closa, Daniel; Bioque, G.; Gelpí, E.; Javerbaum, A.; González, Ezequiel; Gimeno, M.A.F.

doi:10.1016/0021-9673(93)87014-d

Journal of Chromatography A

1993

DOI: 10.1016/0021-9673(93)87014-d

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Liquid chromatography and radioimmunoassay method for the determination of prostaglandins E1 and E2 in rat embryo incubates

Georgina Hotter

Joan Roselló‐Catafau

Daniel Closa

et al.

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Cited by 11 publications

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“…Analysis of prostaglandins (PGs) in plasma, on the other hand, is of crucial importance in the fields of medicine and biochemistry, and a successful method for their microanalysis has been eagerly awaited. Radioimmunoassay (RIA) , and mass spectrometric detection combined with gas chromatographic separation (GC/MS) − have achieved high sensitivity in the several picogram per milliliter region. Laser-induced fluorometric detection combined with high-performance liquid chromatographic separation (HPLC/LIF) is also expected to be a good candidate for sensitive detection of PGs, and several groups have reported microanalysis methods of PGs using HPLC/LIF.…”

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confidence: 99%

In Vivo Determination of Ultratrace Amounts of Prostaglandin in Plasma by High-Performance Liquid Chromatography/Laser-Induced Fluorometry/Ultrasensitive Laser Spectrometry under Severe Conditions

Tsutsumiuchi¹,

Saitô²,

Imagawa³

et al. 1997

Anal. Chem.

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We succeeded in determining ultratrace prostaglandin amounts in plasma, at the femtomolar level, using laser-induced fluorometry through a complete redesign of the analytical procedures. Practical samples, especially plasma, contain large amounts of admixtures, and prostaglandin in plasma (pg/mL) has been considered to be difficult to detect because the samples and reagents supplied by conventional procedures are neither pure nor stable enough to get good results by ultrasensitive laser spectrometry. We completely redesigned the analytical procedures after careful investigations of the reagent purification and the column separation conditions based on a newly found behavior of the reagent and derivatized prostaglandin in a small quantity of ethanol in the mobile phase. A lower determination limit of 23 pg/mL (65 fmol) was achieved, the variance was 12% at 25 pg/mL, and the recovery rate was 88-89%. This method was applied to in vivo analysis of the concentration of prostaglandin E1 administered as a prodrug of prostaglandin E1 (delta(8)-9-O-butyryl prostaglandin F1 butyl ester, AS-013) by intravenous infusion to beagle dogs. A clear correlation between the change of blood pressure and the prostaglandin E1 concentration was confirmed.

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confidence: 99%

In Vivo Determination of Ultratrace Amounts of Prostaglandin in Plasma by High-Performance Liquid Chromatography/Laser-Induced Fluorometry/Ultrasensitive Laser Spectrometry under Severe Conditions

Tsutsumiuchi¹,

Saitô²,

Imagawa³

et al. 1997

Anal. Chem.

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Glucose, glycogen and triglyceride metabolism, as well as prostaglandin production in uterine strips and in embryos from diabetic pregnant rats. Influences of the presence of substrate in the incubation medium

et al. 1993

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Glucose metabolism, triglyceride and glycogen levels, as well as eicosanoid production in isolated uterine strips and in embryos in a rat model of non-insulin-dependent diabetes mellitus during pregnancy

et al. 1994

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Liquid chromatography and radioimmunoassay method for the determination of prostaglandins E1 and E2 in rat embryo incubates

Cited by 11 publications

References 9 publications

In Vivo Determination of Ultratrace Amounts of Prostaglandin in Plasma by High-Performance Liquid Chromatography/Laser-Induced Fluorometry/Ultrasensitive Laser Spectrometry under Severe Conditions

In Vivo Determination of Ultratrace Amounts of Prostaglandin in Plasma by High-Performance Liquid Chromatography/Laser-Induced Fluorometry/Ultrasensitive Laser Spectrometry under Severe Conditions

Glucose, glycogen and triglyceride metabolism, as well as prostaglandin production in uterine strips and in embryos from diabetic pregnant rats. Influences of the presence of substrate in the incubation medium

Glucose metabolism, triglyceride and glycogen levels, as well as eicosanoid production in isolated uterine strips and in embryos in a rat model of non-insulin-dependent diabetes mellitus during pregnancy

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