Glucose metabolism, triglyceride and glycogen levels, as well as eicosanoid production in isolated uterine strips and in embryos in a rat model of non-insulin-dependent diabetes mellitus during pregnancy

Jawerbaum, Alicia; González, Ezequiel; Novaro, Virginia; Gimeno, A.L.; Roselló‐Catafau, Joan; Gómez, G.; Gelpí, E.; Gimeno, M.F.

doi:10.1016/0090-6980(94)90079-5

Cited by 24 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human endometrial tissues expresses both sialidase and sialyltransferase (27,34). In this study, placental sialidase activity is higher in GDM than in normal pregnancy, consistent with the reported abnormal carbohydrate metabolism in the placental-decidual unit of GDM pregnancy (35). Therefore, it is not surprising that the altered carbohydrate metabolism in GDM leads to the production of GdA with reduced sialic acid content.…”

Section: Discussionsupporting

confidence: 92%

Glycosylation Failure Extends to Glycoproteins in Gestational Diabetes Mellitus

et al. 2011

View full text Add to dashboard Cite

OBJECTIVEGestational diabetes mellitus (GDM) is a common metabolic disorder of pregnancy. Patients with GDM are at risk for high fetal mortality and gestational complications associated with reduced immune tolerance and abnormal carbohydrate metabolism. Glycodelin-A (GdA) is an abundant decidual glycoprotein with glycosylation-dependent immunomodulatory activities. We hypothesized that aberrant carbohydrate metabolism in GDM was associated with changes in glycosylation of GdA, leading to defective immunomodulatory activities.RESEARCH DESIGN AND METHODSGdA in the amniotic fluid from women with normal (NGdA) and GDM (DGdA) pregnancies was purified by affinity chromatography. Structural analysis of protein glycosylation was preformed by lectin-binding assay and mass spectrometry. Cytotoxicity, cell death, cytokine secretion, and GdA binding of the GdA-treated lymphocytes and natural killer (NK) cells were determined. The sialidase activity in the placental tissue from normal and GDM patients was measured.RESULTSGDM affected the glycosylation but not the protein core of GdA. Specifically, DGdA had a lower abundance of α2-6–sialylated and high-mannose glycans and a higher abundance of glycans with Sda (NeuAcα2-3[GalNAcβ1-4]Gal) epitopes compared with NGdA. DGdA had reduced immuosuppressive activities in terms of cytotoxicity on lymphocytes, inhibitory activities on interleukin (IL)-2 secretion by lymphocytes, stimulatory activities on IL-6 secretion by NK cells, and binding to these cells. Desialylation abolished the immunomodulation and binding of NGdA. Placental sialidase activity was increased in GDM patients, which may account for the reduced sialic acid content of DGdA.CONCLUSIONSTaken together, this study provides the first direct evidence for altered enzymatic glycosylation and impaired bioactivity of GdA in GDM patients.

show abstract

Section: Discussionsupporting

confidence: 92%

Glycosylation Failure Extends to Glycoproteins in Gestational Diabetes Mellitus

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The second would be alterations in the decidua, as seen in this study, which would delay the synchronism between decidua receptiveness and blastocyst development. Another model of mild diabetes showed a decreased uterine contractility, metabolic abnormalities during pregnancy, and a smaller number of implanted embryos [33, 34], which also corroborates our results even though diabetes induction in our experimental model occurred on the day of birth.…”

Section: Discussionsupporting

confidence: 91%

Neonatally Induced Mild Diabetes in Rats and Its Effect on Maternal, Placental, and Fetal Parameters

Sinzato

Volpato

Iessi

et al. 2012

Experimental Diabetes Research

View full text Add to dashboard Cite

The aim of this study was to assess placental changes and reproductive outcomes in neonatally induced mild diabetic dams and fetal development in their offspring. At birth, female rats were assigned either to control or diabetic group (100 mg of streptozotocin/Kg, subcutaneously). At adulthood, the female rats were mated. During pregnancy, the blood glucose levels and glucose and insulin tolerance tests were performed. At term, maternal reproductive outcomes, fetal and placental weight, and placental morphology were analyzed. Diabetic rats had smaller number of living fetuses, implantations and corpora lutea, and increased rate of embryonic loss. Placenta showed morphometric alterations in decidua area. Our results showed that mild diabetes was sufficient to trigger alterations in maternal organism leading to impaired decidua development contributing to failure in embryonic implantation and early embryonic losses. Regardless placental decidua alteration, the labyrinth, which is responsible for the maternal-fetal exchanges, showed no morphometric changes contributing to an appropriate fetal development, which was able to maintain normal fetal weight at term in mild diabetic rats. Thus, this experimental model of diabetes induction at the day of birth was more effective to reproduce the reproductive alterations of diabetic women.

show abstract

“…There are many reports regarding alterations in the metabolism of arachidonic acid in the cardiovascular system of experimental models of type I or insulin‐dependent diabetes mellitus (IDDM) (Canga, Sterin‐Borda, Borda, Peredo & Gimeno, 1985; Koltai, Hadhazy, Koszeghy, Ballagi‐Pordany & Pogatsa, 1988; Peredo, Filinger, Sanguinetti, Lorenzo & Adler‐Graschinsky, 1994) and in patients with type II or non‐insulin‐dependent diabetes mellitus (NIDDM) (Lasserre et al ., 2000; Okumura, Imanishi, Yanashita, Yamamura, Kim, Iwao, Tanaka & Fujii, 2000); nevertheless, there are no reports of prostanoid production in vascular preparations from experimental models of NIDDM. The NIDDM is the most frequent form of diabetes (Özturk, Altan & Yildizoglu‐Ari, 1996), and the neonatally streptozotocin (STZ) injected rat is a widely used model of this pathology (Bonner‐Weir, Trent, Honey & Weir, 1981; Jawerbaum, Roselló Catafau, González, Franchi, Gelpi, Novaro, Gómez & Gimeno, 1994). The model is characterized by a mild hyperglycaemia between 1 and 20 mmol l –1 , which develops from 6 weeks of age, and little or absent changes in body weight (Jawerbaum, Roselló Catafau, González, Franchi, Gelpi, Novaro, Gómez & Gimeno, 1995; Hemmings & Spafford, 2000).…”

Section: Introductionmentioning

confidence: 99%

Prostanoid release and constrictor responses to noradrenaline in the rat mesenteric vascular bed in non‐insulin‐dependent diabetes mellitus

Peredo

2001

Journal of Autonomic Pharmacology

View full text Add to dashboard Cite

1. The administration of streptozotocin (STZ) to 2-day old rats induced a non-insulin-dependent diabetes mellitus (NIDDM)-like state, with mild hyperglycaemia and no alterations in body weight at the adult age. 2. In the isolated and perfused mesenteric vascular bed of NIDDM animals, the constrictor responses to either noradrenaline (NA) or potassium chloride (KCl) were not modified as compared with age-matched non-diabetic controls. 3. The reduction in NA contractions induced by the cyclooxygenase inhibitor, 10 microM indomethacin in the control group was absent in the NIDDM rats. 4. The increase in the NA-induced contractions caused by endothelium removal was suppressed by indomethacin in the controls but not in the NIDDM group. 5. The prostanoid release from the mesenteric vascular beds of NIDDM rats was markedly reduced as compared with non-diabetic controls. Noradrenaline increased production of the constrictor prostaglandin (PG) F2alpha in control but not in NIDDM rats. 6. In summary, these results show that in STZ-induced NIDDM rats, there is an impairment of the prostanoid production, as well as a suppression of the role of prostanoids in the contractile effects of NA in the mesenteric vascular bed. These alterations are more severe than those previously observed in a model of insulin-dependent diabetes mellitus (IDDM), in which hyperglycaemia and reduction of body weight were more marked. The conclusion is that, in these models of diabetes and in the preparation studied, vascular alterations and modifications of glycaemia and body weight are not closely related.

show abstract

Glucose metabolism, triglyceride and glycogen levels, as well as eicosanoid production in isolated uterine strips and in embryos in a rat model of non-insulin-dependent diabetes mellitus during pregnancy

Cited by 24 publications

References 28 publications

Glycosylation Failure Extends to Glycoproteins in Gestational Diabetes Mellitus

Glycosylation Failure Extends to Glycoproteins in Gestational Diabetes Mellitus

Neonatally Induced Mild Diabetes in Rats and Its Effect on Maternal, Placental, and Fetal Parameters

Prostanoid release and constrictor responses to noradrenaline in the rat mesenteric vascular bed in non‐insulin‐dependent diabetes mellitus

Contact Info

Product

Resources

About