Liquid chromatographic separation of derivatives of diospyrin, a bioactive bisnaphthoquinonoid plant-product, and analogous naphthyl compounds

Sanyal, Utpal; Bhattacharyya, S.; Patra, Amarendra; Hazra, Banasri

doi:10.1016/j.chroma.2003.08.008

Cited by 22 publications

(13 citation statements)

References 22 publications

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“…This is significant from the phytochemical perspective since all analytical reports on Ventilago sp. had shown it to be a rich source of hydroxyanthraquinonoid compounds, which would be extracted better with chloroform rather than with the more polar solvents (Sanyal et al, 2003;Hazra et al, 2004). In the present study also, done for the first time on the stem-bark of this species, two major products isolated from the bioactive fractions were found to be hydroxyanthraquinonoids, namely emodin and physcion (Fig.…”

Section: Discussionmentioning

confidence: 77%

Evaluation of the antibacterial activity ofVentilago madraspatana Gaertn.,Rubia cordifolia Linn. andLantana camara Linn.: isolation of emodin and physcion as active antibacterial agents

2005

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The antibacterial activity of the extracts of Ventilago madraspatana stem-bark, Rubia cordifolia root and Lantana camara root-bark, prepared with solvents of different polarity, was evaluated by the agar-well diffusion method. Twelve bacteria, six each of gram-positive and gram-negative strains, were used in this study. Chloroform and ethanol extracts of V. madraspatana showed broad-spectrum activity against most of the bacteria except S. aureus, E. coli and V. cholerae. On the other hand, the activity of the chloroform and methanol extracts of R. cordifolia and L. camara was found to be more specific towards the gram-positive strains, although gram-negative P. aeruginosa was also inhibited by the methanol extracts of both these plants in a dose dependent manner. The water extracts of V. madraspatana and L. camara were found to be inactive, while that of R. cordifolia was significantly active against B. subtilis and S. aureus compared with streptomycin and penicillin G used as standards. In the course of bio-assay guided fractionation, emodin and physcion were isolated for the first time from the stem-bark of V. madraspatana. It was noteworthy to find the MICs of emodin in the range 0.5-2.0 microg/mL against three Bacillus sp. Both the anthraquinonoid compounds inhibited P. aeruginosa, emodin being more effective, showing an MIC of 70 microg/mL.

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Section: Discussionmentioning

confidence: 77%

Evaluation of the antibacterial activity ofVentilago madraspatana Gaertn.,Rubia cordifolia Linn. andLantana camara Linn.: isolation of emodin and physcion as active antibacterial agents

2005

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“…A técnica de CLAE apresenta-se com relativa facilidade de manipulação e permite ampla análise de diferentes compostos. A análise por CLAE deve considerar a necessidade de condições ótimas de separação, o que depende da fase móvel utilizada, pH, pressão da bomba, preparo da amostra etc (Sanyal et al, 2003;Thanawiroon & Linhardt, 2003;Liang et al, 2004).…”

Section: Procedimentosunclassified

O Brasil no contexto de controle de qualidade de plantas medicinais

Souza-Moreira¹,

Salgado²,

Pietro³

2010

Rev. bras. farmacogn.

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RESUMO:Atualmente, as plantas medicinais movem altos valores financeiros em todo o mundo e representam o tipo de tratamento mais acessível para cerca de 80% da população, principalmente entre os países em desenvolvimento. Entretanto, existe ainda uma falta de conhecimento sobre propriedades químicas, farmacológicas e toxicológicas a fim de assegurar a eficácia e segurança das plantas medicinais. Os critérios de eficácia e segurança de plantas medicinais estão relacionados a qualidade, isto é, as plantas necessitam ser corretamente identificadas, cultivadas e coletadas, devem estar livres de material estranho, partes de outras plantas e contaminações inorgânicas e/ ou microbianas. O objetivo deste estudo foi o de elucidar os diferentes processos e padronizações sobre o controle de drogas vegetais, principalmente no Brasil.Unitermos: Plantas medicinais, fitoquímica, controle de qualidade.ABSTRACT: "Brazil in the context of plants and derivates quality control". Nowadays medicinal plants move high financial values at the world and represent a type of treatment more accessible for about 80% of people, mainly among developing countries. However, there is a lack of knowledge about chemical, pharmacological and toxicological properties towards ensure their efficacy and safety. Efficacy and safety criteria of medicinal plants are related to quality, that is, the plants need to be correctly identified, cultivated and harvested, they must be free of strange material, part of other plants and inorganic and/or microorganisms contamination. The aim of this study was to elucidate different process and standardizations about quality control of vegetal drugs mainly in Brazil. ISSN 0102-695X Keywords INTRODUÇÃOPor longos períodos na história, o uso de plantas medicinais foi presente não só devido ao seu caráter alimentar, mas também às suas propriedades de cura, sejam elas reais ou ritualísticas. Entretanto, até a metade do século XX, o uso de medicamentos de origem sintética tornou-se mais amplo que o uso de plantas medicinais, principalmente na sociedade ocidental, com destaque para os países desenvolvidos visto que os considerados países em desenvolvimento permaneceram fazendo uso das propriedades curativas das plantas, certamente devido às difíceis condições econômicas de aquisição dos medicamentos sintéticos

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“…D1 and its derivatives D2, D3, and D7 were synthesized and purified as described previously (Hazra et al, 1984;Sanyal et al, 2003). The syntheses of D16, D17, and D18 are to be described in the doctoral dissertation of M. Das Sarma at Jadavpur University (unpublished).…”

Section: Methodsmentioning

confidence: 99%

Selective Inhibition of Topoisomerase I and Various Steps of Spliceosome Assembly by Diospyrin Derivatives

Tazi¹,

Bakkour²,

Soret³

et al. 2004

Mol Pharmacol

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Pre-mRNA splicing is an essential step of the expression of most metazoan protein-coding genes, which is often regulated in a cell type-specific or developmental manner. We have demonstrated previously that human DNA topoisomerase I, an extensively studied target for anticancer drugs, also has an intrinsic protein kinase activity that specifically phosphorylates proteins involved in splice site selection. Therefore, DNA topoisomerase I was recently shown to play a critical role in alternative splicing. Here, we have exploited these novel properties of DNA topoisomerase I to develop entirely novel diospyrin derivatives targeting its protein kinase activity and thereby modulating pre-mRNA splicing. Although some derivatives indeed inhibit kinase activity of topoisomerase I, they did not block reactions of topoisomerase I on DNA. However, these drugs interfere with camptothecin-dependent topoisomerase I-mediated DNA cleavage, implying that diospyrin derivatives mediate a conformational change of topoisomerase I. It is noteworthy that in vitro splicing reactions revealed that diospyrin derivatives alter various steps of splicing. Some diospyrin derivatives inhibit either the first or the second catalytic step of splicing but not spliceosome assembly, whereas diospyrin itself prevents the formation of full spliceosome. Our data revealed for the first time that diospyrin derivatives are able to stall the dynamic assembly of the spliceosome and open the exciting possibility of using these derivatives to correct aberrant splicing in human genetic diseases.

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Liquid chromatographic separation of derivatives of diospyrin, a bioactive bisnaphthoquinonoid plant-product, and analogous naphthyl compounds

Cited by 22 publications

References 22 publications

Evaluation of the antibacterial activity ofVentilago madraspatana Gaertn.,Rubia cordifolia Linn. andLantana camara Linn.: isolation of emodin and physcion as active antibacterial agents

Evaluation of the antibacterial activity ofVentilago madraspatana Gaertn.,Rubia cordifolia Linn. andLantana camara Linn.: isolation of emodin and physcion as active antibacterial agents

O Brasil no contexto de controle de qualidade de plantas medicinais

Selective Inhibition of Topoisomerase I and Various Steps of Spliceosome Assembly by Diospyrin Derivatives

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