1988
DOI: 10.1016/s0378-4347(00)83109-6
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Liquid chromatographic determination of taurine in whole blood, plasma and platelets

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Cited by 35 publications
(21 citation statements)
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“…Free and total plasma TRP levels were determined using a high-performance liquid chromatographic-fluorometric system after the addition of 5-hydroxytryptophan internal standard (final concentration 5 mg/ml) and perchloric acid by direct injection (Anderson et al, 1981). Both free and total plasma TRP levels were determined with within-assay and assay-to-assay coefficients of variation of less than 5 and 10%, respectively.…”
Section: Trp and Hormone Assaysmentioning
confidence: 99%
“…Free and total plasma TRP levels were determined using a high-performance liquid chromatographic-fluorometric system after the addition of 5-hydroxytryptophan internal standard (final concentration 5 mg/ml) and perchloric acid by direct injection (Anderson et al, 1981). Both free and total plasma TRP levels were determined with within-assay and assay-to-assay coefficients of variation of less than 5 and 10%, respectively.…”
Section: Trp and Hormone Assaysmentioning
confidence: 99%
“…AMPT was measured by reverse phase HPLC with fluorescence detection. The procedure used was adapted from that of Anderson et al (1981) for tyrosine and tryptophan. Sample preparation was similar to that originally used by Engelman et al (1968) to measure plasma AMPT, except that following protein precipitation we used HPLC to enable separation of AMPT from tyrosine.…”
Section: Ampt Plasma Analysismentioning
confidence: 99%
“…This confirms previous observations made using IIPLC 7 • .1(1. 41 but, more importantly, extends the finding to include the spectrofluorimctric procedure. ':' Studies using other proccduresf" 4ll have demonstrated marked decreases in serotonin level assayed with increasing time of storage.…”
Section: Methods Comparisons and Analytical Variablesmentioning
confidence: 62%
“…Parallel IIPLC determinations in this study were run both with and without carbon monoxide pre-treatment as recommended by Korpi''" and Anderson et al 41 respectively. The excellent correlation we obtained between the two HPLC procedures, therefore, contradicts a previous observation by Korpi'" that direct protein precipitation with concentrated perchloric acid totally destroys serotonin in whole blood and that degradation cannot be prevented simply by using EDTA and ascorbic acid as suggested previously," 44.…”
Section: Methods Comparisons and Analytical Variablesmentioning
confidence: 99%
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