Liquid Chromatographic Determination of Inositol Hexanicotinate in Formulated Preparations

Abstract

A precise and selective liquid chromatographic procedure for determining inositol hexanicotinate (IHN) in formulated preparations was developed and validated. IHN was dissolved in dimethyl sulfoxide, diluted with acetonitrile, chromatographed on a Nova-Pak C18 column with a mobile phase of water-methanol (40 + 60, v/v), and detected at 262 nm. The correlation coefficient of the calibration curve was 0.9995 over the range 0.050.15 mg/mL. Overall recovery of IHN was >97%. Coefficients of variation for intra- and interday precisions were <2%.

Pharmaceuticals and Related Drugs

“…Besides these, two books have been published that cover general aspects of the chromatographic analysis of pharmaceuticals ( , ) and reviews have appeared that consider more specific chromatographic topics such as drug purity (), quality control (), and validation (). The popularity of capillary electrophoretic methods continues to expand as do the number articles that deal with either experimental ( − ) or validation ( − ) issues. Other technique-related reviews have been concerned with flow injection analysis (), ion-selective electrodes ( , ), ESI and MALDI mass spectrometry (), scanning probe microscopy (), thermoanalytical methods (), the analysis of antibiotics by LC/MS (), and the characterization of polymorphs and solvates by vibrational spectroscopy and nuclear magnetic resonance spectroscopy ().…”