Liquid Chromatographic Determination of Atorvastatin in Bulk Drug, Tablets, and Human Plasma

Altuntas, T. Gul; Erk, Nevin

doi:10.1081/jlc-120027087

Cited by 65 publications

(30 citation statements)

References 9 publications

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“…The plasma samples was mixed with acetonitrile in a 1:1 ratio for proteins precipitation, the supernatant was separated by centrifugation at 8000 rpm for 5 min. Results of a previous study showed that acetonitrile is the best reagent to precipitate proteins (29). Our observations revealed that an overlap between ATC and a background plasma peak could be resolved by modifications of solvent composition and pH of the mobile phase.…”

Section: Hplc Analysismentioning

confidence: 65%

“…The concentrations of ATC in rat plasma samples were determined using a modified RP-HPLC method developed by Altuntas and Erk (29). The plasma samples was mixed with acetonitrile in a 1:1 ratio for proteins precipitation, the supernatant was separated by centrifugation at 8000 rpm for 5 min.…”

Section: Hplc Analysismentioning

confidence: 99%

“…Different methods were proposed for determination of ATC in biological fluids in the literature (19,28,29). The concentrations of ATC in rat plasma samples were determined using a modified RP-HPLC method developed by Altuntas and Erk (29).…”

Section: Hplc Analysismentioning

confidence: 99%

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Coamorphous Atorvastatin Calcium to Improve its Physicochemical and Pharmacokinetic Properties

et al. 2013

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-Purpose: Atorvastatin calcium (ATC) is classified as class II (low solubility and high permeability) compound according to the biopharmaceutical classification system. The amorphous form of ATC possesses higher solubility, dissolution rate, and bioavailability than its crystalline form. Coamorphous drug system is a new and emerging method to prepare stable amorphous forms, in this case leading to the improved stability of ATC in dissolution medium. Methods: In this study, coamorphous form of ATC and nicotinamide (ATC-NIC) was prepared from solvent evaporation method and characterized using differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR) and powder X-ray diffraction (PXRD). The intrinsic dissolution rate and solubility of ATC-NIC were determined along with plasma concentrations of ATC using HPLC after oral dosing in rats. Results: The crystalline ATC was converted to coamorphous form revealing a molecular interaction between ATC and NIC. The intrinsic dissolution rate, solubility and plasma concentration of coamorphous ATC-NIC are higher than those of crystalline ATC. ATC-NIC coamorphous system showed greater solution stability than those reported in the literature for amorphous ATC. Conclusions: Coamorphous ATC-NIC has improved physicochemical and pharmacokinetic properties as compared to ATC.

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Section: Hplc Analysismentioning

confidence: 65%

Section: Hplc Analysismentioning

confidence: 99%

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Coamorphous Atorvastatin Calcium to Improve its Physicochemical and Pharmacokinetic Properties

et al. 2013

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“…Several methods have already been reported concerning the determination of atorvastatin in pharmaceutical formulations and clinical samples, including the HPLC system, [5][6][7][8][9][10][11][12] spectrophotometry, 13,14 micellar liquid chromatographic, 15 densitometry 16 and reverse-phase HPLC. 17 Electrochemical techniques, such as differential pulse voltammetry (DPV) using modified or unmodified electrodes, can be considered for the determinations of pharmaceutical compounds as strong alternatives to the other instrumental methods.…”

Section: Introductionmentioning

confidence: 99%

Polytetrafluorethylene Film-Based Liquid-Three Phase Micro Extraction Coupled with Differential Pulse Voltammetry for The Determination of Atorvastatin Calcium

2013

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In this paper, we describe a new combination method based on polytetrafluorethylene (PTFE) film-based liquid threephase micro extraction coupled with differential pulse voltammetry (DPV) for the micro extraction and quantification of atorvastatin calcium (ATC) at the ultra-trace level. Different factors affecting the liquid-three phases micro extraction of atorvastatin calcium, including organic solvent, pH of the donor and acceptor phases, concentration of salt, extraction time, stirring rate and electrochemical factors, were investigated, and the optimal extraction conditions were established. The final stable signal was achieved after a 50 min extraction time, which was used for analytical applications. An enrichment factor of 21 was achieved, and the relative standard deviation (RSD) of the method was 4.5% (n = 4). Differential pulse voltammetry exhibited two wide linear dynamic ranges of 20.0 -1000.0 pmol L -1 and 0.001 -11.0 μmol L -1 of ATC. The detection limit was found to be 8.1 pmol L -1 ATC. Finally, the proposed method was used as a new combination method for the determination of atorvastatin calcium in real samples, such as human urine and plasma.

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“…Atorvastatin calcium {[R-(R, R*)]-2-(4-flurophenyl)-β,δ-dihydroxy-5(1-methylethyl)-3-phenyl-4-[phenylamino)carbonyl]-1H-pyrrole-1-heptanoic acid, calcium salt (2:1) trihydrate} is the most commonly occurring drug in commercially available pharmaceutical formulations used for the clinical treatment of hypercholesterolemia [1]. Several methods have been described for the quantitative determination of atorvastatin by high-performance liquid chromatography (HPLC) in different pharmaceutical preparations, either alone [2][3][4][5][6][7] or with other active ingredients [8][9][10][11][12][13][14][15][16]. HPTLC [17], electrochemical [18,19], capillary electrophoresis [20] and spectrofluorimetric [21] methods have been developed for the analysis of atorvastatin from its individual and combined formulations with other active ingredients in pharmaceutical preparations and plasma.…”

Section: Introductionmentioning

confidence: 99%

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

Ashour¹

2013

Pharmaceut Anal Acta

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New, accurate, sensitive and reliable kinetic spectrophotometric method for the assay of atorvastatin calcium (AVS) in pure form and pharmaceutical formulations has been developed. The method involves the oxidative coupling reaction of AVS with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) in the presence of Ce(IV) in an acidic medium to form colored product with lambdamax at 566 nm. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 566 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 2.0-20.0 µg/mL for initial rate and fixed time methods. The limit of detection for initial rate and fixed time methods is 0.093 and 0.064 µg/mL, respectively. Molar absorptivity for the method was found to be 3.36×10 4 L/ mol cm. Statistical treatment of the experimental results indicates that the methods are precise and accurate. Both methods have been applied successfully for the estimation of pravastatin sodium in commercial dosage forms with no interference from the excipients. The results are compared with the pharmacopoeial method.

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Liquid Chromatographic Determination of Atorvastatin in Bulk Drug, Tablets, and Human Plasma

Cited by 65 publications

References 9 publications

Coamorphous Atorvastatin Calcium to Improve its Physicochemical and Pharmacokinetic Properties

Coamorphous Atorvastatin Calcium to Improve its Physicochemical and Pharmacokinetic Properties

Polytetrafluorethylene Film-Based Liquid-Three Phase Micro Extraction Coupled with Differential Pulse Voltammetry for The Determination of Atorvastatin Calcium

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

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