New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

Abstract

New, accurate, sensitive and reliable kinetic spectrophotometric method for the assay of atorvastatin calcium (AVS) in pure form and pharmaceutical formulations has been developed. The method involves the oxidative coupling reaction of AVS with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) in the presence of Ce(IV) in an acidic medium to form colored product with lambdamax at 566 nm. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 566 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 2.0-20.0 µg/mL for initial rate and fixed time methods. The limit of detection for initial rate and fixed time methods is 0.093 and 0.064 µg/mL, respectively. Molar absorptivity for the method was found to be 3.36×10 4 L/ mol cm. Statistical treatment of the experimental results indicates that the methods are precise and accurate. Both methods have been applied successfully for the estimation of pravastatin sodium in commercial dosage forms with no interference from the excipients. The results are compared with the pharmacopoeial method.

Kinetics, thermodynamics, and DFT studies of phenols oxidative coupling reactions with 4-amino-N, N-dimethylaniline

“…To show stability of the compound this study has been performed, which is ranging between (14.36 -20.26)L/mol , the stubility was followed by taking (A) at different periods: (2,4,6,12,24,48,72)hours , the stubility was constant within that times.…”

“…The study of kinetic techniques involves measuring the progress of reactions across a wide range of scales, from chemical bond interactions between atoms and electrons to chemical production rates in reactors. Kinetic approaches have numerous advantages, including high selectivity methods that assess absorbance as a function of reaction time rather than a specific absorbance value (12). They also provide simple and fast procedures, skipping several experimental stages such as filtration and extraction before absorbance measurements.…”

Spectrophotometric Determination of Atorvastatin Calcium and Rosuvastatin Calcium in Bulk and Dosage Form Using P-Dimethylaminobenzaldehyde

“…It acts by inhibiting the enzyme 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-co A) reductase [2]. Several analytical methods, including spectrophotometric methods [3][4][5][6][7], spectrofluorometric [8], chromatographic methods [9][10][11][12][13][14][15][16] and potentiometric method [17] have already been reported for its determination, either alone or in combination with other drugs. Rosuvastatin calcium is chemically, [(E) -(3R, 5S)-7-{4-(4-fluorophenyl)-6-isopropyl-2-{(methyl (methane sulfonyl amino)] pyrimidin-5-yl}-3, 5-dihydroxyhepten-6-oic acid, calcium salt] Figure 1.…”

New Kinetic Spectrophotometric Method for Determination of Fexofenadine Hydrochloride in Pharmaceutical Formulations

“…The literature is still poor in analytical assay methods based on kinetics for the determination of fexofenadine in dosage forms. Some specific advantages that the kinetic methods possess are as follows: simple and fast methods because some experimental steps such as filtration and extraction are avoided prior to absorbance measurements; high selectivity since they involve the measurement of the absorbance as a function of reaction time instead of measuring the concrete absorbance value; other active compounds present in the commercial dosage forms which may not interfere if they are resisting the chemical reaction conditions established for the proposed kinetic method, and colored and/or turbid sample background which may not possibly interfere with the determination process [29,30].…”