Liquid chromatographic analysis of metoclopramide with fluorescence detection in cirrhotic patients

Abstract

Metoclopramide extracted from plasma and urine has been analysed using reversed phase HPLC with acetic acid/acetonitrile/triethylamine as eluent and fluorescence detection. This method exploits the natural fluorescence of metoclopramide for its detection in patients on multiple drug therapies.

Sensitive and selective liquid chromatography–tandem mass spectrometry method for the determination of metoclopramide in human plasma: application to a bioequivalence study

“…The reported bioanalytical methods for the quantifi cation of MCP in biological fl uids were based on either gas chromatography/mass spectrometry (Bateman et al, 1978;Jones et al, 1993;Riggs et al, 1994) or high-performance liquid chromatography with ultra violet detection (Graff ner et al, 1979;Popovic, 1984;Riley, 1984;de Jong et al, 1987;Albani et al, 1987;Takahashi et al, 1987;Fairhead et al, 1989;Buss et al, 1990;Lamparczyk et al, 2001), fl uorescence detection (Horiuchi et al, 2006;Vlase et al, 2006) and electrochemical detection (Zaki et al, 2006). The major disadvantages of all these methods include the use of a large amount of biological samples (0.5-2.0 mL plasma or urine samples), complicated extraction procedures or long chromatographic run time.…”

Determination of metoclopramide in human plasma by LC–ESI-MS and its application to bioequivalance studies

“…The techniques adopted include gas chromatography-mass spectrometry (GC-MS) [2][3][4], electrogenerated chemiluminescence (ECL) [5] and high performance liquid chromatography (HPLC) methods with UV [6][7][8][9][10][11][12][13][14], fluorescence [15] or electrochemical detection [16]. The above methods have poor sensitivity and specificity for the determination of metoclopramide in plasma after administration of low dosages.…”

Determination of metoclopramide in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry

“…Normal-phase HPLC using a silica column [6,7] and reversed-phase (RP) HPLC [9,10,[12][13][14][15][16] or ion-pair HPLC methods on octadecyl column [8,11,17] were described for the analysis of metoclopramide in biological fluids. To the best of our knowledge, the LC/MS method for the determination of metoclopramide in biological fluids has not been described.…”

“…For the quantification of metoclopramide in biological fluids, gas chromatography/mass spectrometry (GC/MS) [3][4][5] and high performance liquid chromatography (HPLC) methods with UV [6][7][8][9][10][11][12][13][14], fluorescence [15,16] or electrochemical detection [17] have been reported. Normal-phase HPLC using a silica column [6,7] and reversed-phase (RP) HPLC [9,10,[12][13][14][15][16] or ion-pair HPLC methods on octadecyl column [8,11,17] were described for the analysis of metoclopramide in biological fluids.…”

“…To the best of our knowledge, the LC/MS method for the determination of metoclopramide in biological fluids has not been described. The sample preparation procedures for the extraction of metoclopramide from biological matrix consisted of protein precipitation [16], solid-phase extraction [9], liquid-liquid extraction (LLE) [3][4][5][6][7][8]10,11,[13][14][15], on-line trace enrichment [12] or combination of protein precipitation with SPE [17]. Those methods use a large amount of biological samples (0.5-2.0 mL plasma or urine samples) in order to obtain the high sensitivity or include relatively time-consuming extraction procedures and long analysis time.…”