Determination of metoclopramide in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry

Lee, Hye Won; Ji, Hye Young; Kim, Hoe Yoon; Park, Eun-Seok; Lee, Kang Choon; Lee, Hye Suk

doi:10.1016/j.jchromb.2009.04.027

Cited by 37 publications

(16 citation statements)

References 28 publications

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“…The LOD and LOQ of our method were found to be 0.02 and 0.05 ng/ml, and the signal-to-noise ratios of the LOD and LOQ were 3 and 10, respectively. The LOD and LOQ were more sensitive than other previous reports using Lc-MS/MS (LOQ: 0.78 ng/ml [16], 2 ng/ml [12]). …”

Section: Lc-ms/ms Methodmentioning

confidence: 53%

Section: Lc-ms/ms Methodmentioning

confidence: 86%

“…Selection of the IS was based on the conditions of the Lc-MS/MS method reported by Lee et al [12]. The SRM monitor ions selected in this study for the determination of McP and IS were similar to those of Lee et al [12]. Figure 1 shows a representative Lc-MS/MS chromatogram of standard (100 ng/ml, A), blank plasma spiked with McP, the plasma 15 min following a single intravenous administration of McP and blank plasma.…”

Section: Lc-ms/ms Methodmentioning

confidence: 99%

“…have been reported in humans and goats after administration of McP [2, 7,8], as this agent readily crosses the blood-brain barrier [6]. Although there is a large amount a pharmacokinetic data available in humans [3,4,[12][13][14]16], there is little data about the McP concentration in serum and urine of cattle [9][10][11]. According to the pharmacokinetic study in Angus cows reported by Jones et al [11], McP was administered intravenously at a dose of 4 mg/kg.…”

Section: Metoclopramide (Mcp: 4 Amino-5-chloro-n-(2-(diethylamino)ethmentioning

confidence: 99%

“…Analytical methods for the determination of McP in blood samples include high performance liquid chromatography with a UV detector (HPLc-UV) [4,8,9,11,13,14], Gc-MS [3,10,15] and Lc-tandem mass spectroscopy (Lc-MS/MS) [12,16]. Of these methods, the Lc-MS/MS method has a highest detection sensitivity in human plasma, e.g., 0.78 ng/ ml (limit of quantitation: LOQ) according to Yan et al [16].…”

Section: Metoclopramide (Mcp: 4 Amino-5-chloro-n-(2-(diethylamino)ethmentioning

confidence: 99%

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Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography

Hamamoto

Mizuno

Kato

et al. 2013

The Journal of Veterinary Medical Science

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AbSTRAcT. A quantitative assay method using Lc/ESI-MS/MS for simultaneous determination of McP in cattle plasma was developed and validated. chromatographic separation was carried out using a multimode column (2 × 150 mm, 3 µm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). McP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5-500 ng/ml (R 2 >0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96-103%, and the coefficient of variation was less than 6.5%. Plasma MCP concentrations after intravenous administration at 0.4 mg/kg to 12 cattle were determined by the Lc-MS/MS method.

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Section: Lc-ms/ms Methodmentioning

confidence: 53%

Section: Lc-ms/ms Methodmentioning

confidence: 86%

Section: Lc-ms/ms Methodmentioning

confidence: 99%

Section: Metoclopramide (Mcp: 4 Amino-5-chloro-n-(2-(diethylamino)ethmentioning

confidence: 99%

Section: Metoclopramide (Mcp: 4 Amino-5-chloro-n-(2-(diethylamino)ethmentioning

confidence: 99%

See 3 more Smart Citations

Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography

Hamamoto

Mizuno

Kato

et al. 2013

The Journal of Veterinary Medical Science

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Sensitive and selective liquid chromatography–tandem mass spectrometry method for the determination of metoclopramide in human plasma: application to a bioequivalence study

Inamadugu

Dodda

Mullangi

et al. 2010

Biomedical Chromatography

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A simple, sensitive and rapid method has been developed and validated for determination of the metoclopramide (MCP) in 100 microL human plasma. The analytical procedure involves a liquid-liquid extraction method using tramadol as an internal standard (IS). Chromatographic separation was carried out on a HyPURITY ADVANCE column using a mobile phase consisting of acetonitrile and 10 mm ammonium acetate buffer in the ratio of 80:20 (v/v) at a flow rate of 0.3 mL/min. The total run time of analysis was 2.5 min and elution of MCP and IS occurred at 0.9 and 1.3 min, respectively. A linear response function was established for the range of concentrations 0.53-42.07 ng/mL (r > 0.99). The intra- and inter-day precision values for MCP met the acceptance as per FDA guidelines. MCP was stable in a battery of stability studies viz., bench-top, auto-sampler and freeze-thaw cycles. The developed assay method was successfully applied to an oral bioequivalence study in humans.

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High Performance Liquid Chromatography for the Determination of Metoclopramide in Pharmaceutical Preparations Using Pre‐column Derivatization with Fluorescamine

Ulu

2012

Chin. J. Chem.

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A simple, rapid and sensitive high performance liquid chromatography (HPLC) method was developed for the determination of metoclopramide in pharmaceutical preparation. The method is based on the derivatization of metoclopramide with fluorescamine. The separation was achieved on a C18 column using methanol‐water (70:30, V/V) mobile phase. Fluorescence detector was used at the excitation and emission of 403 and 485 nm, respectively. The method was validated for linearity, limit of detection, limit of quantification, precision, accuracy, recovery, robustness and system suitability. The assay was linear over the concentration range of 100–2000 ng/mL. The mean recovery was 100.37%. The proposed method was successfully applied to the assay of metoclopramide in tablet preparation. The preparation was also analyzed with an official method and statistical comparison by t‐ and F‐tests revealed that there was no significant difference between the results of the two methods with respect to mean values and standard deviations at the 95% confidence level.

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Determination of metoclopramide in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry

Cited by 37 publications

References 28 publications

Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography

Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography

Sensitive and selective liquid chromatography–tandem mass spectrometry method for the determination of metoclopramide in human plasma: application to a bioequivalence study

High Performance Liquid Chromatography for the Determination of Metoclopramide in Pharmaceutical Preparations Using Pre‐column Derivatization with Fluorescamine

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