Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

Abstract

Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. However, its molecular mechanisms andpharmacological targets are not clear. In this study, the ultra-performance liquid tandem mass spectrometry-based metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism. A total of 25 biomarkers associated with the prostatitis model were identified by metabolomics, and a number of metabolic pathways closely related to the model were obtained by MetPA analysis. After given Mag treatment, the results of each indicator were shown that Mag alkaloid could inhibit the development of prostatitis effectively. We found that Mag had regulative effects on potential biomarkers of prostatitis model, which can regulate them to the control group. Our results indicated that alkaloids have an effective intervention therapy for prostatitis, and five types of metabolic pathways closely related to prostatitis model were obtained, including phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tyrosine metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism. This study has provided the basic experimental data for the development of Mag in the prevention and treatment of prostatitis.

Preclinical models and evaluation criteria of prostatitis

“…Chronic prostatitis was produced by direct chemical injection, including l-carrageenan (131-133), capsaicin (134)(135)(136), and doxycycline (40). This method was assigned to help study the neurobiological mechanisms of male pelvic pain, although it caused damage, destruction, or degeneration of the prostates.…”

The Age-Accompanied and Diet-Associated Remodeling of the Phospholipid, Amino Acid, and SCFA Metabolism of Healthy Centenarians from a Chinese Longevous Region: A Window into Exceptional Longevity

“…First, the two complementary strategies, non-targeted metabolomics and metabolite target analysis were used. Non-targeted metabolomics is a valuable approach to obtaining a comprehensive depiction of the metabolic status closely related to the phenotypic outcome of interest in an unbiased manner [ 38 ]. Meanwhile, targeted metabolomics, which is an accurate quantitative method to analyze biochemically known and annotated metabolites, provides information that is more precise on specific metabolites and metabolic pathways [ 39 ].…”