Liposomes/Chitosan Scaffold/Human Fibrin Gel Composite Systems for Delivering Hydrophilic Drugs—Release Behaviors of TirofibanIn Vitro

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

“…The ESCA analysis provided further evidence of the presence of NGF in PCL-NGF scaffolds. Interestingly, an S 2p signal (0.1%, Table 1) was obtained from the PCL-NGF/TF scaffold, because of the presence of sulfur molecules in TF only [23]. Although increases in the intensity of the O peak in the ESCA spectra of PCL-NGF/TF scaffolds may have been caused by the presence of carboxyl groups in TF (Table 1), the peaks of sulfur molecules in the ESCA spectra revealed TF in the PCL-NGF/TF scaffolds.…”

“…Although increases in the intensity of the O peak in the ESCA spectra of PCL-NGF/TF scaffolds may have been caused by the presence of carboxyl groups in TF (Table 1), the peaks of sulfur molecules in the ESCA spectra revealed TF in the PCL-NGF/TF scaffolds. Moreover, a semi-quantitative HPLC analysis of the grafting efficiency of TF into PCL-NGF/TF scaffolds performed [13,23]. The grafting efficiency of TF into PCL scaffolds was about 70% of the initial concentration.…”

“…For a semi-quantitative analysis of the grafting efficiency of TF to PCL scaffolds, the solution used for washing samples after photochemical reactions, was collected, and further analyzed by an HPLC (Jasco PU-1580, Kobe, Japan) equipped with a C18 reverse phase column (#201SP54, 4.6 mm_25 cm ID, VyDAC Corp., Hesperia, CA, USA) at room temperature as mentioned in our early reports [13,23]. For this analysis, 3% acetonitrile dissolved in 0.1% of TFA solution was used as the mobile phase with 0.7 ml/min flow rate, and the quantity of TF-SANPAH was detected by absorption intensity of wavelength at 220 nm that was analyzed with built-in standard software of the HPLC [13,23].…”

“…For this analysis, 3% acetonitrile dissolved in 0.1% of TFA solution was used as the mobile phase with 0.7 ml/min flow rate, and the quantity of TF-SANPAH was detected by absorption intensity of wavelength at 220 nm that was analyzed with built-in standard software of the HPLC [13,23]. …”

Low molecular weight chitosan-coated liposomes for ocular drug delivery:In vitroandin vivostudies

“…Another hopeful biomaterial is chitosan, which also has been intensively investigated for ocular drug delivery (Ludwig et al, 2005;Diebold et al, 2007;Wang et al, 2008;Abdelbary, 2011). Low molecular weight chitosan (LCH) is a derivate from chitosan which achieves a marvelous improvement in water-solubility when comparing it with its precursor (Li et al, 2005).…”