Low molecular weight chitosan-coated liposomes for ocular drug delivery:<i>In vitro</i>and<i>in vivo</i>studies

Li, Ning; Zhuang, Chun-Yang; Wang, Mi; Sui, Chengguang; Pan, Wei-San

doi:10.3109/10717544.2011.621994

Cited by 58 publications

(29 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unfortunately, the preparation of liposomes is associated with disadvantages because of the instability, degradability and aggregation of the liposomes and with limitations in drug loading, particularly for hydrophilic drugs . One approach to overcome these disadvantages in enabling administration to the eye is dispersing the liposomes in polymeric formulations . One of the more recent investigations that used this strategy stabilised flurbiprofen‐containing cationic liposomes within a thermosensitive gel that formed in situ .…”

Section: Delivery Systemsmentioning

confidence: 99%

Topical delivery of ocular therapeutics: carrier systems and physical methods

Souza

Dias

Pereira

et al. 2013

Journal of Pharmacy and Pharmacology

114

View full text Add to dashboard Cite

Objective The basic concepts, major mechanisms, technological developments and advantages of the topical application of lipid-based systems (microemulsions, nanoemulsions, liposomes and solid lipid nanoparticles), polymeric systems (hydrogels, contact lenses, polymeric nanoparticles and dendrimers) and physical methods (iontophoresis and sonophoresis) will be reviewed. Key findings Although very convenient for patients, topical administration of conventional drug formulations for the treatment of eye diseases requires high drug doses, frequent administration and rarely provides high drug bioavailability. Thus, strategies to improve the efficacy of topical treatments have been extensively investigated. In general, the majority of the successful delivery systems are present on the ocular surface over an extended period of time, and these systems typically improve drug bioavailability in the anterior chamber whereas the physical methods facilitate drug penetration over a very short period of time through ocular barriers, such as the cornea and sclera. Summary Although in the early stages, the combination of these delivery systems with physical methods would appear to be a promising tool to decrease the dose and frequency of administration; thereby, patient compliance and treatment efficacy will be improved.

show abstract

Section: Delivery Systemsmentioning

confidence: 99%

Topical delivery of ocular therapeutics: carrier systems and physical methods

Souza

Dias

Pereira

et al. 2013

Journal of Pharmacy and Pharmacology

114

View full text Add to dashboard Cite

show abstract

“…Moreover, to solve the problem of stabilization of bioactive agents, one may increase the rigidity of the liposome membrane, while concurrently decreasing their tendency for aggregation by properly selecting the liposome lipid components [12]. The use of a thin polymeric coating made of chitosan or alginate may be a strategy to increase the stability of bioactive agents into the liposomes [87][88][89]. However, the polymeric coatings may increase the size of the liposomes and may also interact with the phospholipid bilayer.…”

Section: Hydrophilic Bioactive Agentsmentioning

confidence: 99%

Liposomes in tissue engineering and regenerative medicine

Monteiro

Martins

Reis

et al. 2014

J. R. Soc. Interface.

314

217

View full text Add to dashboard Cite

Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications in cosmetics and tissue engineering. Tissue engineering is defined as a strategy for promoting the regeneration of tissues for the human body. This strategy may involve the coordinated application of defined cell types with structured biomaterial scaffolds to produce living structures. To create a new tissue, based on this strategy, a controlled stimulation of cultured cells is needed, through a systematic combination of bioactive agents and mechanical signals. In this review, we highlight the potential role of liposomes as a platform for the sustained and local delivery of bioactive agents for tissue engineering and regenerative medicine approaches.

show abstract

“…9 Coating of anionic liposomes with CS and its derivatives via electrical attraction may result in charge reversal on their surface. 10 Liposomes coated with CS or TMC maintain the capability to open tight junctions, improve absorption, and further prolong the residence time in GIT, 11 and thus enhance the oral bioavailability. Nowadays, Caco-2 cell monolayers have been widely used to study the absorption, cellular uptake, cytotoxicity, and metabolism and evaluate the behavior of oral drugs in intestines.…”

Section: Introductionmentioning

confidence: 99%

Liposomes coated with N-trimethyl chitosan to improve the absorption of harmine in vivo and in vitro

Chen¹,

Yuan²,

Liu³

et al. 2016

IJN

View full text Add to dashboard Cite

In this study, harmine liposomes (HM-lip) were prepared through the thin-film hydration–pH-gradient method and then coated with N -trimethyl chitosan (TMC). Particle size, zeta potential, entrapment efficiency, and in vitro release of HM-lip and TMC-coated harmine liposomes (TMC-HM-lip) were also determined. Sprague Dawley rats were further used to investigate the pharmacokinetics in vivo. Retention behavior in mouse gastrointestinal tract (GIT) was studied through high-performance liquid chromatography and near-infrared imaging. Degradation was further evaluated through incubation with Caco-2 cell homogenates, and a Caco-2 monolayer cell model was used to investigate the uptake and transport of drugs. HM-lip and TMC-HM-lip with particle size of 150–170 nm, an entrapment efficiency of about 81%, and a zeta potential of negative and positive, respectively, were prepared. The release of HM from HM-lip and TMC-HM-lip was slower than that from HM solution and was sensitive to pH. TMC-HM-lip exhibited higher oral bioavailability and had prolonged retention time in GIT. HM-lip and TMC-HM-lip could also protect HM against degradation in Caco-2 cell homogenates. The uptake amount of TMC-HM-lip was higher than that of HM and HM-lip. TMC-HM-lip further demonstrated higher apparent permeability coefficient ( P app ) from the apical to the basolateral side than HM and HM-lip because of its higher uptake and capability to open tight junctions in the cell monolayers. TMC-HM-lip can prolong the retention time in the GIT, protect HM against enzyme degradation, and improve transport across Caco-2 cell monolayers, thus enhancing the oral bioavailability of HM.

show abstract

Low molecular weight chitosan-coated liposomes for ocular drug delivery:In vitroandin vivostudies

Cited by 58 publications

References 29 publications

Topical delivery of ocular therapeutics: carrier systems and physical methods

Topical delivery of ocular therapeutics: carrier systems and physical methods

Liposomes in tissue engineering and regenerative medicine

Liposomes coated with N-trimethyl chitosan to improve the absorption of harmine in vivo and in vitro

Contact Info

Product

Resources

About