Liposome–polyethylenimine complexes for enhanced DNA and siRNA delivery

Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells

“…This suggests that cationic lipids formed an outer coat around the preformed polyplexes, in agreement with the "core-shell" structure of lipopolyplexes proposed by earlier studies. 21,22 Nevertheless, it should be noted that the surface charge of lipopolyplexes also increased with increasing PEI/ DNA ratio. Thus, it is possible that the polyplex core was not entirely wrapped by the lipids, as shown in Scheme 1.…”

“…PEI/DNA polyplexes in combination with liposomes containing DOCSPER, 19 DOSPER, 19 DOTAP, 20 DOTMA, 21 DPPC, 22 or DPPG 22 have been shown to improve the transfection of immortal cell lines under serum conditions. Several parameters, in particular the lipid structure and vector/DNA molar ratio, were suggested to be factors influencing the transfection behavior of the lipopolyplexes.…”

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

“…For instance, coating of adenovirus with polymers or liposomes allows the production of "stealth" viruses that can avoid recognition by the host's antibodies and permits targeting of desired receptors following linkage of ligands to the chemical coating Kim et al, 2010;Zhong et al, 2010). Other hybrid vector combinations include polymer-artificial chromosome (Magin-Lachmann et al, 2004); polymer-gold nanoparticle (Thomas & Klibanov, 2003b); liposome-peptide-artificial chromosome (White et al, 2003); polymerpeptide , liposome-polymer (Schäfer et al ,2010) and viral vector combinations such as adenovirus-Epstein-Barr virus (Gardlík et al, 2005). The general consensus is that viral gene deliver systems achieve stable in vivo transgene expression more efficiently than non-viral systems (Gardlík et al, 2005;Hassam et al, 2009;Escoffre et al, 2010;Grigsby & Leong, 2010).…”

Photo-Enhanced Delivery of Genetic Material Using Curcumin Loaded Composite Nanocarriers

“…Poly Ethylen Imine (PEI) on the other hand is a proven polymer for gene delivery and is often considered as a gold standard for transfection [14][15][16]. A combination of PEI/nucleic acid polyplexes and liposomes called Lipopolyplexes (LPP) has been proven to increase the efficiency of gene delivery and decrease the toxicity associated with polyethylenimine [17][18][19]. In this study, we present the use of a novel combination of curcumin loaded liposomes and PEI/DNA polyplexes for photochemical internalisation.…”