Liposome as a Delivery System for Carotenoids: Comparative Antioxidant Activity of Carotenoids As Measured by Ferric Reducing Antioxidant Power, DPPH Assay and Lipid Peroxidation

Tan, Chen; Xue, Jingyi; Abbas, Shabbar; Feng, Bo; Zhang, Xiaoming; Xia, Shuqin

doi:10.1021/jf405622f

Cited by 163 publications

(77 citation statements)

References 42 publications

(84 reference statements)

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“…In vitro DPPH radical scavenging kinetics of the formulated flavonosomes through various methods were screened in comparison to the carrier (PC), freely mixed flavonoids (QKA), and physical mixture (QKA + PC), according to the protocol adapted from previous reports 28,29 with slight modifications. Briefly, 150 µL of formulated flavonosomes or PC or QKA or QKA + PC was mixed with 50 µL of 0.1 mM DPPH solution (methanol with 0.3% Triton X-100 v/v).…”

Section: In Vitro Antioxidant Dpph Kineticsmentioning

confidence: 99%

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Karthivashan¹,

Kura²,

Abas³

et al. 2016

IJN

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This study involves adaptation of bulk or sequential technique to load multiple flavonoids in a single phytosome, which can be termed as “flavonosome”. Three widely established and therapeutically valuable flavonoids, such as quercetin (Q), kaempferol (K), and apigenin (A), were quantified in the ethyl acetate fraction of Moringa oleifera leaves extract and were commercially obtained and incorporated in a single flavonosome (QKA–phosphatidylcholine) through four different methods of synthesis – bulk (M1) and serialized (M2) co-sonication and bulk (M3) and sequential (M4) co-loading. The study also established an optimal formulation method based on screening the synthesized flavonosomes with respect to their size, charge, polydispersity index, morphology, drug–carrier interaction, antioxidant potential through in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics, and cytotoxicity evaluation against human hepatoma cell line (HepaRG). Furthermore, entrapment and loading efficiency of flavonoids in the optimal flavonosome have been identified. Among the four synthesis methods, sequential loading technique has been optimized as the best method for the synthesis of QKA–phosphatidylcholine flavonosome, which revealed an average diameter of 375.93±33.61 nm, with a zeta potential of −39.07±3.55 mV, and the entrapment efficiency was >98% for all the flavonoids, whereas the drug-loading capacity of Q, K, and A was 31.63%±0.17%, 34.51%±2.07%, and 31.79%±0.01%, respectively. The in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics of the flavonoids indirectly depicts the release kinetic behavior of the flavonoids from the carrier. The QKA-loaded flavonosome had no indication of toxicity toward human hepatoma cell line as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide result, wherein even at the higher concentration of 200 µg/mL, the flavonosomes exert >85% of cell viability. These results suggest that sequential loading technique may be a promising nanodrug delivery system for loading multiflavonoids in a single entity with sustained activity as an antioxidant, hepatoprotective, and hepatosupplement candidate.

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Section: In Vitro Antioxidant Dpph Kineticsmentioning

confidence: 99%

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Karthivashan¹,

Kura²,

Abas³

et al. 2016

IJN

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“…Nanosized carriers, such as oil drops, polymers, lipoproteins, liposomes and micelles allow one to prepare water dispersions of carotenoids with enhanced stability (Hou, Liu, Lei, & Gao, 2014;Toniazzo et al, 2014;Tan et al, 2014;Ascenso et al, 2013;Luxsuwong, Indranupakorn, & Wongtrakul, 2014;Donhowe & Kong, 2014;Acevedo et al, 2014;Bustos-Garza, Yanez-Fernandez, & Barragan-Huerta, 2013).…”

Section: Introductionmentioning

confidence: 99%

Water soluble biocompatible vesicles based on polysaccharides and oligosaccharides inclusion complexes for carotenoid delivery

Polyakov

Kispert

2015

Carbohydrate Polymers

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Since carotenoids are highly hydrophobic, air- and light-sensitive hydrocarbon compounds, developing methods for increasing their bioavailability and stability towards irradiation and reactive oxygen species is an important goal. Application of inclusion complexes of "host-guest" type with polysaccharides and oligosaccharides such as arabinogalactan, cyclodextrins and glycyrrhizin minimizes the disadvantages of carotenoids when these compounds are used in food processing (colors and antioxidant capacity) as well as for production of therapeutic formulations. Cyclodextrin complexes which have been used demonstrated enhanced storage stability but suffered from poor solubility. Polysaccharide and oligosaccharide based inclusion complexes play an important role in pharmacology by providing increased solubility and stability of lipophilic drugs. In addition they are used as drug delivery systems to increase absorption rate and bioavailability of the drugs. In this review we summarize the existing data on preparation methods, analysis, and chemical reactivity of carotenoids in inclusion complexes with cyclodextrin, arabinogalactan and glycyrrhizin. It was demonstrated that incorporation of carotenoids into the "host" macromolecule results in significant changes in their physical and chemical properties. In particular, polysaccharide complexes show enhanced photostability of carotenoids in water solutions. A significant decrease in the reactivity towards metal ions and reactive oxygen species in solution was also detected.

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“…The ferric ion reducing antioxidant power, commonly referred to as the FRAP assay is one of several methods widely employed for the determination of the antioxidant properties of many natural products (Müller et al, 2011;Sudan et al, 2014;Tan et al, 2014).…”

Section: Antioxidant Activities As Determined By Frap Assaymentioning

confidence: 99%

Amelioration of Lead-induced Toxicity in Blood, Liver and Kidney Tissues of Male Wistar Rats by Fermented Ofada Rice

Aganbi

Avwioroko²,

Enabulele³

et al. 2015

Turkish JAF Sci.Tech.

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The protective effects of 'ofada' rice koji (ORK, fermented ofada rice) and ascorbic acid (AA) against lead (Pb)-induced toxicity in the blood, liver and kidney tissues of male Wistar rats was investigated. The animals were divided into four treatment groups (A -D, n = 5). Groups B, C and D were intoxified by intra-peritoneal injection of 75 mg lead acetate/kg body weight. Groups C and D only had their feed mixed with ORK and AA, respectively. The results showed no significant difference in % packed cell volume (PCV) and Pb concentrations. Feeding with ORK and AA significantly decreased alanine aminotransferase activities (36.50 ± 3.54 and 34.02 ± 0.05 UL -1 respectively) compared to Pb-only treated group (85.50 ± 3.25 UL -1 ). The ferric reducing antioxidant power (FRAP) for organs increased significantly following intake of feeds mixed with ORK and AA; increases in FRAP was higher for ORK-treated group possibly due to increased total flavonoids concentration following fermentation. Furthermore, Pb-induced high plasma creatinine levels decreased upon treatment with feeds mixed with ORK and ascorbic acid. These findings strongly indicated that feed supplementation with ORK by 45% may be more effective at ameliorating the effects of Pb-induced toxicity in tissues compared to supplementation with AA by 2%.

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Liposome as a Delivery System for Carotenoids: Comparative Antioxidant Activity of Carotenoids As Measured by Ferric Reducing Antioxidant Power, DPPH Assay and Lipid Peroxidation

Cited by 163 publications

References 42 publications

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Water soluble biocompatible vesicles based on polysaccharides and oligosaccharides inclusion complexes for carotenoid delivery

Amelioration of Lead-induced Toxicity in Blood, Liver and Kidney Tissues of Male Wistar Rats by Fermented Ofada Rice

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