Lipopolysaccharide-responsive phosphoproteins in Nicotiana tabacum cells

Gerber, Isak B.; Laukens, Kris; Witters, Erwin; Dubery, Ian A.

doi:10.1016/j.plaphy.2006.06.015

Cited by 48 publications

(32 citation statements)

References 69 publications

(55 reference statements)

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“…Bacterial strains may be identified as pathogens, triggering a cascade of immediate defensive response (Gerber et al, 2006;Millet et al, 2010) or, in some cases, they may be detected as a potential non-aggressive pathogen, and gently trigger secondary metabolism, a priming response (Conrath et al, 2002, van Hulten et al, 2006. When the bacteria are able to prime the plant, secondary metabolism are stimulated in such a way that upon subsequent stress challenge the defensive response will be more intense than in non-primed plants.…”

Section: Research Articlementioning

confidence: 99%

Elicitation of secondary metabolism inHypericum perforatumby rhizosphere bacteria and derived elicitors in seedlings and shoot cultures

Mañero

Algar

Gómez

et al. 2012

Pharmaceutical Biology

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Section: Research Articlementioning

confidence: 99%

Elicitation of secondary metabolism inHypericum perforatumby rhizosphere bacteria and derived elicitors in seedlings and shoot cultures

Mañero

Algar

Gómez

et al. 2012

Pharmaceutical Biology

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“…Although it was suggested that RuBPS delivered superior sensitivity in comparison to SR [163], it was later demonstrated that there was no quantitative advantage over the original or optimized SR formulation [164]. It is, however, definitely more cost-effective and has thus been used in various proteomic investigations [165][166][167][168][169][170]. It has been shown that the limit of sensitivity of RuBPS is approximately 10 ng protein/band (broad-range MW standards, Bio-Rad), as reported previously [164] and determined qualitatively from published data [163]; however, a more detailed evaluation of RuBPS staining indicated this sensitivity threshold to be much lower, at ∼2 ng protein/band (CA, soybean trypsin inhibitor, OVA, albumin, PhosB) [168].…”

Section: Non-reactive Fluorescent Dyesmentioning

confidence: 99%

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

2010

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Proteomics research relies heavily on visualization methods for detection of proteins separated by polyacrylamide gel electrophoresis. Commonly used staining approaches involve colorimetric dyes such as Coomassie Brilliant Blue, fluorescent dyes including Sypro Ruby, newly developed reactive fluorophores, as well as a plethora of others. The most desired characteristic in selecting one stain over another is sensitivity, but this is far from the only important parameter. This review evaluates protein detection methods in terms of their quantitative attributes, including limit of detection (i.e., sensitivity), linear dynamic range, inter-protein variability, capacity for spot detection after 2D gel electrophoresis, and compatibility with subsequent mass spectrometric analyses. Unfortunately, many of these quantitative criteria are not routinely or consistently addressed by most of the studies published to date. We would urge more rigorous routine characterization of stains and detection methodologies as a critical approach to systematically improving these critically important tools for quantitative proteomics. In addition, substantial improvements in detection technology, particularly over the last decade or so, emphasize the need to consider renewed characterization of existing stains; the quantitative stains we need, or at least the chemistries required for their future development, may well already exist.

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“…Using LPS of Burkholderia cepacia, the induction of several early signaling and defense pathways in tobacco has been confirmed (Gerber et al, 2004). Recent studies have identified several tobacco proteins altered in their phosphorylation status in response to elicitation with B. cepacia LPS, giving valuable insights into possible signaling processes that follow the perception of the PAMP (Gerber et al, 2006). LPS are also important signal molecules in symbiotic signaling.…”

mentioning

confidence: 94%

The Lipopolysaccharide of Sinorhizobium meliloti Suppresses Defense-Associated Gene Expression in Cell Cultures of the Host Plant Medicago truncatula

et al. 2007

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In the establishment of symbiosis between Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti, the lipopolysaccharide (LPS) of the microsymbiont plays an important role as a signal molecule. It has been shown in cell cultures that the LPS is able to suppress an elicitor-induced oxidative burst. To investigate the effect of S. meliloti LPS on defenseassociated gene expression, a microarray experiment was performed. For evaluation of the M. truncatula microarray datasets, the software tool MapMan, which was initially developed for the visualization of Arabidopsis (Arabidopsis thaliana) datasets, was adapted by assigning Medicago genes to the ontology originally created for Arabidopsis. This allowed functional visualization of gene expression of M. truncatula suspension-cultured cells treated with invertase as an elicitor. A gene expression pattern characteristic of a defense response was observed. Concomitant treatment of M. truncatula suspension-cultured cells with invertase and S. meliloti LPS leads to a lower level of induction of defense-associated genes compared to induction rates in cells treated with invertase alone. This suppression of defense-associated transcriptional rearrangement affects genes induced as well as repressed by elicitation and acts on transcripts connected to virtually all kinds of cellular processes. This indicates that LPS of the symbiont not only suppresses fast defense responses as the oxidative burst, but also exerts long-term influences, including transcriptional adjustment to pathogen attack. These data indicate a role for LPS during infection of the plant by its symbiotic partner.

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Lipopolysaccharide-responsive phosphoproteins in Nicotiana tabacum cells

Cited by 48 publications

References 69 publications

Elicitation of secondary metabolism inHypericum perforatumby rhizosphere bacteria and derived elicitors in seedlings and shoot cultures

Elicitation of secondary metabolism inHypericum perforatumby rhizosphere bacteria and derived elicitors in seedlings and shoot cultures

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

The Lipopolysaccharide of Sinorhizobium meliloti Suppresses Defense-Associated Gene Expression in Cell Cultures of the Host Plant Medicago truncatula

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