Lipopolysaccharide-mediated enhancement of zymosan phagocytosis by RAW 264.7 macrophages is independent of opsonins, laminarin, mannan, and complement receptor 3

Fuentes, Ana-Lucía; Millis, Leonard M.; Vapenik, Jacqueline; Sigola, Lynette B.

doi:10.1016/j.jss.2014.03.024

Cited by 29 publications

(21 citation statements)

References 56 publications

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“…On the other hand, phagocytic capacity was measured as the mean number of particles per phagocytic cell. Phagocytic index (PI) or phagocytic activity was determined employing the equation (17,18):…”

Section: Phagocytosis Assaymentioning

confidence: 99%

Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals

Park

Abihssira-García

Thalmann³

et al. 2020

Front. Immunol.

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Imaging flow cytometry (IFC) is a powerful tool which combines flow cytometry with digital microscopy to generate quantitative high-throughput imaging data. Despite various advantages of IFC over standard flow cytometry, widespread adoption of this technology for studies in aquatic sciences is limited, probably due to the relatively high equipment cost, complexity of image analysis-based data interpretation and lack of core facilities with trained personnel. Here, we describe the application of IFC to examine phagocytosis of particles including microplastics by cells from aquatic animals. For this purpose, we studied (1) live/dead cell assays and identification of cell types, (2) phagocytosis of degradable and non-degradable particles by Atlantic salmon head kidney cells and (3) the effect of incubation temperature on phagocytosis of degradable particles in three aquatic animals-Atlantic salmon, Nile tilapia, and blue mussel. The usefulness of the developed method was assessed by evaluating the effect of incubation temperature on phagocytosis. Our studies demonstrate that IFC provides significant benefits over standard flow cytometry in phagocytosis measurement by allowing integration of morphometric parameters, especially while identifying cell populations and distinguishing between different types of fluorescent particles and detecting their localization.

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Section: Phagocytosis Assaymentioning

confidence: 99%

Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals

Park

Abihssira-García

Thalmann³

et al. 2020

Front. Immunol.

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show abstract

“…The endocytic index (EI) was calculated by the mean number of ingested particles (MNP) and percentage of cells with ingest particles (PCIP) as EI = MNP Â PCIP. 26 Cells were imaged at Â63 magnification using Scanner Zeiss Axio Imager Z2 (Carl Zeiss, Jena, Germany) and Metafer 4/VSlide (Metasystems, Altlussheim, Germany) software. Images were analyzed using ImageJ software.…”

Section: Macrophage Assaysmentioning

confidence: 99%

Differential effects of Zincum metallicum on cell models

et al. 2017

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“…27,[29][30][31][32][33] GPs uptake has been demonstrated to be dectin-1 dependent in vitro. [34][35][36][37][38] The ability of GPs to serve as carrier relies upon the possibility to ll up the cavity with chemicals with different physico-chemical properties.…”

Section: 27-29mentioning

confidence: 99%

Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis

et al. 2015

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Lipopolysaccharide-mediated enhancement of zymosan phagocytosis by RAW 264.7 macrophages is independent of opsonins, laminarin, mannan, and complement receptor 3

Cited by 29 publications

References 56 publications

Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals

Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals

Differential effects of Zincum metallicum on cell models

Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis

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