Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblasts

Abstract

These results demonstrate that P. nigrescens lipopolysaccharide stimulates osteoclastogenesis in the coculture system by decreasing the production of OPG and increasing the production of TGF-beta and PGE2. Through the mechanisms involving these factors, P. nigrescens lipopolysaccharide may cause alveolar bone resorption in periodontal diseases.

Antigenic Activity of Bacterial Endodontic Contents from Primary Root Canal Infection with Periapical Lesions against Macrophage in the Release of Interleukin-1β and Tumor Necrosis Factor α

“…The high frequency of P. nigrescens in primary endodontic infection with apical periodontitis seems to be related to its LPS potential in causing bone resorption (24). The almost exclusive presence of P. endodontalis in endodontic infections suggests a specific association with bone resorption and activation of macrophages cells (5,25).…”

Antigenicity of Primary Endodontic Infection against Macrophages by the Levels of PGE2 Production

“…According to previous investigations (21,22), the elevated level of endotoxin was not only related to exudation (positively associated with the number of gram-negative bacterial species) but also to the presence of P. nigrescens bacteria and/or concomitant infection by both P. endodontalis and F. nucleatum. The high frequency of P. nigrescens in endodontic infection seems to be related to its LPS toxicity (29). Teeth with exudation had been previously associated with F. nucleatum (3).…”

“…In addition, P. nigrescens recovered from almost all teeth with pain on palpation was shown to possess a very potent LPS molecule for PGE 2 stimulation (18,29).…”

New Insights into Prevotella Diversity and Medical Microbiology

“…Moreover, it has been shown recently that leptin, a protein mainly synthesized by adipocytes, may enhance P. intermedia LPS-induced TNF-a production by macrophages [101]. P. intermedia LPS has been shown to reduce alkaline phosphatase activity and mineralization, and to induce IL-6 and metalloproteinases in fetal mouse osteoblasts, contributing to the inhibition of bone formation and the enhancement of osteolysis [102,103]. LPS of P. nigrescens and of P. intermedia may also contribute to tissue destruction by stimulating the production of nitric oxide in host cells [103,104].…”