Lipopolysaccharide-binding protein is associated with arterial stiffness in patients with type 2 diabetes: a cross-sectional study

Abstract

BackgroundLipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase reactant that mediates immune responses triggered by LPS. Recent evidence indicates the association of circulating LBP levels with obesity, diabetes, and cardiovascular diseases. In this study, we aimed to investigate the relationship between serum LBP levels and arterial stiffness in patients with type 2 diabetes.MethodsA total of 196 patients with type 2 diabetes, including 101 men and 95 women, were enrolled in this cross-sectional study. Fasting serum LBP levels were determined by enzyme-linked immunosorbent assay. Arterial stiffness was assessed by measuring the aortic pulse wave velocity (PWV).ResultsThe mean values of serum LBP and aortic PWV were 18.2 μg/mL and 1194 cm/s, respectively. Serum LBP levels were positively correlated with body mass index, triglycerides, high-sensitivity C-reactive protein, and insulin resistance index and were negatively correlated with high-density lipoprotein cholesterol. They were, however, not significantly correlated with aortic PWV in univariate analyses. Multivariate analysis revealed that serum LBP levels were independently and positively associated with aortic PWV (β = 0.135, p = 0.026) after adjusting for age, sex, body mass index, albumin, high-sensitivity C-reactive protein, and other cardiovascular risk factors. Further analyses revealed that the impact of serum LBP levels on aortic PWV was modified by sex, and the association between serum LBP levels and aortic PWV was found to be significant only in men.ConclusionsSerum LBP levels are associated with arterial stiffness, independent of obesity and traditional cardiovascular risk factors, especially in men with type 2 diabetes. This study indicates a potential role of the LPS/LBP-induced innate immunity in the development and progression of arterial stiffness in type 2 diabetes.

Bacterial translocation markers in type 2 diabetes mellitus: their association with glycemic control and diabetic kidney disease in Egyptian patients

“…Furthermore, circulating plasma LBP levels were significantly and positively associated with BMI, systolic blood pressure, aspartate aminotransferase, alanine aminotransferase, total cholesterol, LDL-C, fasting glucose and insulin, and insulin resistance in the study of Moreno-Navarrete et al [9]. Serum LBP levels were also positively correlated with the parameters of obesity, insulin resistance, and inflammation in diabetic subjects as mentioned in a previous Japanese study [23]. In a prospective observational study involving 49 obese subjects undergoing bariatric surgery and 17 controls, plasma LPS was positively correlated with cardiometabolic risk factors, including triglycerides, systolic blood pressure, and BMI and was negatively correlated with HDL cholesterol [24].…”

Serum Lipopolysaccharide-Binding Protein is Associated with Chronic Inflammation and Metabolic Syndrome in Hemodialysis Patients

“…On the contrary, it might be argued that the presence of elevated levels of LBP protein remains an indirect clue to the role of endotoxin. Nevertheless previous studies [28][29] suggested that LBP is a useful parameter for assessing the biological activity of circulating endotoxin in patient blood. Over the years, few studies have reliably identified individuals who suffer the frequent passage of bacteria or their products into circulation because measurement of endotoxin in biological fluids is notoriously difficult.…”

Association of insulin resistance, from mid-life to late-life, with aortic stiffness in late-life: the Atherosclerosis Risk in Communities Study

“…Recent reports identified associations of aortic stiffness with dysregulation in various metabolic pathways in the setting of type 2 diabetes. Treatment with an incretin mimetic was observed to improve carotidfemoral PWV in individuals with newly diagnosed type 2 diabetes [37] and serum levels of the acute phase reactant lipopolysaccharide-binding protein were shown to be associated with aortic PWV in patients with type 2 diabetes, especially in men [38]. Administration of sodium glucose co-transporter 2 inhibitor reduced aortic stiffness in type 2 diabetic female mice (db/db) [39].…”