Lipolysis, fatness, gender and plasma leptin concentrations in healthy, normal-weight subjects

Morio, Béatrice; Gachon, A.M.; Boirie‌, Yves; Rousset, Paulette; Gachon, Pierre; Beaufrère, B.; Ritz, Patrick

doi:10.1007/s003940050041

Cited by 10 publications

(6 citation statements)

References 42 publications

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“…There is only one clinical study that revealed a positive correlation between glycerol concentrations and plasma leptin. 19 However, this observation could be due to an indirect effect of leptin. Our experiments clearly indicate that leptin has no significant effect on basal and isoproterenol-induced lipolysis in subcutaneous adipocytes from both lean and obese individuals.…”

Section: Discussionmentioning

confidence: 99%

Effects of leptin on the differentiation and metabolism of human adipocytes

et al. 2001

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BACKGROUND:Leptin is an adipose protein regulating food intake in the hypothalamus. Animal studies have suggested that leptin also acts in an auto-=paracrine fashion on adipose cell function. OBJECTIVE: The aim of this study was to investigate the effects of leptin on the differentiation and metabolism of cultured human adipocytes. MATERIAL: Adipose tissue from young healthy, lean women (body mass index (BMI) < 27 kg=m 2 ) undergoing elective mammary reduction surgery and young obese individuals (BMI > 40 kg=m 2 ) undergoing laparoscopic gastric banding. METHODS: Human preadipocytes in primary culture were induced to undergo differentiation by defined adipogenic factors. Mature adipocytes were isolated by collagenase digestion and kept in culture suspension. Glycero-3-phosphate dehydrogenase (GPDH) activity was used as a marker of adipose differentiation; glucose uptake, lipolysis and PAI-1 secretion were measured as parameters of fat cell function. RESULTS: Human preadipocytes and adipocytes from lean and obese subjects expressed the long leptin receptor isoform and two of the three short forms as assessed by polymerase chain reaction (PCR). Leptin at a supraphysiological concentration induced a transient increase of GPDH activity, but had no effect on glucose uptake and PAI-1 secretion from human adipocytes. In addition, basal and isoproterenol-stimulated lipolysis as well as the antilipolytic action of insulin in human adipocytes was not significantly affected by leptin exposure. CONCLUSION: In contrast to animal data, the results of our experiments do not demonstrate significant effects of leptin on main metabolic functions of human adipocytes arguing against a local auto-=paracrine action of leptin in human adipose tissue.

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Section: Discussionmentioning

confidence: 99%

Effects of leptin on the differentiation and metabolism of human adipocytes

et al. 2001

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“…There are only few studies available that have investigated associations of plasma concentrations and these studies found contradictory results. One study in healthy adults found a weak and positive relationship between plasma leptin and plasma NEFA which, however, was only statistically significant in univariate analysis [ 33 ]. Other studies exploring the effect of leptin injections in animals, found that leptin reduced plasma NEFA levels in female rats [ 34 ] but increased in pigs [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics reveals an entanglement of fasting leptin concentrations with fatty acid oxidation and gluconeogenesis in healthy children

Kirchberg

Brandt²,

Moß³

et al. 2017

PLoS ONE

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BackgroundLeptin and adiponectin communicate with organ systems in order to regulate energetic and metabolic homeostasis. Their different points of action have been well characterized; however, no study has investigated their interrelationship with the metabolism at the molecular level in vivo.ObjectiveTo examine the associations of leptin and adiponectin with the metabolic profile reflecting the intercellular and interorgan communication as well as activated metabolic pathways.Patients/MethodsWe measured plasma concentrations of leptin, adiponectin, and insulin along with concentrations of 196 metabolites in 400 healthy, fasting 8-years old German children who participated in the German Ulm Birth Cohort Study (UBCS). Using multiple linear mixed models, we evaluated the associations between hormones and metabolites.ResultsLeptin levels increased exponentially with increasing BMI. Leptin was furthermore strongly associated with alanine and aspartate (Bonferroni corrected P[PBF] = 5.7×10−8 and 1.7×10−6, respectively), and negatively associated to the sum of the non-esterified fatty acids (NEFA) and the sum of the long-chain acylcarnitines C12–C18 (PBF = 0.009 and 0.0001, respectively). Insulin showed a similar association pattern, although the associations were less strong than for leptin. Adiponectin was neither related to BMI nor to any metabolite.ConclusionAlthough children were presumably metabolically similar, we found strong associations of insulin and leptin with the metabolite profile. High alanine concentrations and the lower concentrations of NEFA in children with high fasting leptin concentrations might arise from an increased gluconeogenesis and from the disinhibiting effect of leptin on the carnitine-palmitoyltransferase-1, respectively. As insulin had the same trend towards these associations, both hormones seem to be related to processes that provide the body with energy in fasting state.

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“…Yapılan çalışmalar serum leptin düzeylerinin sağ-lıklı yetişkin ve çocuklarda olduğu gibi KBY hastalarında da VKİ ile anlamlı korelasyona sahip olduğunu göstermiştir (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) . Çalışmamızda, KBY hasta grubunda cinsiyet ayırımı yapmaksızın kontrol grubunda olduğu gibi VKİ ile serum leptin düzeyleri arasında anlamlı pozitif korelasyon olduğunu bulduk.…”

Section: Discussionunclassified

Serum Leptin Concentration In Children With Chronic Renal Failure

Berksoy¹,

Alpay²,

Yıldız³

et al. 2018

BUCH

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ÖZAmaç: Üremik iştahsızlıkta çeşitli faktörler sorumlu tutulmaktadır. Serum leptin düzeylerinde artış ve üremik toksinler melanokortin hormon reseptörlerini uyararak enerji tüketimini artırıp, yiyecek alımını azaltırlar. Bu çalışmada, kronik böbrek yetmezliği olan çocuklar ile sağlıklı kontrollerin serum leptin konsantrasyonlarını karşılaştırarak leptinin böbrek yolu ile atılımını araştırdık. Yöntem: Kronik böbrek yetmezliği nedeniyle takip edilen toplam 33 çocuk hasta ve kontrol grubunu oluşturan 29 sağlıklı çocuğun serum leptin, leptin/VKİ, albümin, glikoz, kreatinin, üre, trigliserid ve kolesterol düzeylerini değerlendirdik. Bulgular: Kız hastalarda ortalama serum leptin düzeyi (18,49±28,55), leptin/VKİ (0,94±1,36) kontrol grubu leptin (7,73±5,65) ve leptin/VKİI (0,94±1,36) değerlerine göre yüksekti (p>0,05). Erkek hastalarda ise serum leptin düzeyi (1,48±1,36; 4,88±3,70) ve leptin/VKİ kontrole göre (0,08±0,081; 0,25±0,16) düşük bulundu (p<0,01). Kızlarda hasta ve sağlıklı grupta serum leptin düzeyi VKİ ile anlamlı pozitif korelasyon gösterir-ken, erkek hasta grubunda leptin ile VKİ arasında korelasyon bulunmadı. Diyaliz erkek hasta grubu dışındaki tüm hastalarda leptin ile glomeruler filtrasyon hızı ve serum kreatin düzeyleri arasında korelasyon yoktu. Serum leptin düzeyleri ile yaş, hastalık tanı süresi, diyaliz süresi arasında da korelasyon bulamadık. Sonuç: Serum leptin düzeyleri ile böbrek fonksiyonları arasında bir ilişkinin saptanmaması ve renal yetmezliği olan erkek hastalarda serum leptin konsantrasyonlarının sağlıklı erkek çocuklardan anlamlı olarak düşük bulunması, leptinin veya reseptör proteinlerinin sentezi veya etkinliğinde ya da böbrek dışı atılımında rol oynayan başka etkenlerin de bulunduğunu düşündürmektedir.Anahtar kelimeler: Çocuk, kronik böbrek yetmezliği, leptin ABSTRACT Objective: Various factors are responsible for uremic anorexia. Increase in serum leptin levels and uremic toxins stimulate melanocortin hormone receptors to increase energy consumption and reduce food intake. In this study, we investigated renal excretion of leptin by comparing serum leptin concentrations of healthy controls, and children with chronic renal failure. Methods: Serum leptin, leptin / BMI, albumin, glucose, creatinine, urea, triglyceride and cholesterol levels were eveluated in a total of 33 pediatric patients followed up for chronic renal failure and 29 healthy children in the control group. Results: Serum leptin levels (18.49±28.55) and leptin / BMI (0.94±1.36) in female patients were higher than leptin (7.73±5.65) and leptin / BMI (0.39±0.25) in the control group (p>0.05). In male patients, serum leptin levels (1.48±1.36; 4.88±3.70) and leptin/ BMI (0.08±0.081; 0.25±0.16) were found to be lower than those of the control group (p<0,01). Serum leptin levels showed a significant positive correlation with BMI in female patients with chronic renal failure and, healthy women; whilethere was no correlation between leptin and BMI in the male patient group. There was no correlation between leptin and glomerular ...

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Lipolysis, fatness, gender and plasma leptin concentrations in healthy, normal-weight subjects

Abstract: Adiposity, lipolysis, and gender are related to plasma leptin in healthy humans.

Cited by 10 publications

References 42 publications

Effects of leptin on the differentiation and metabolism of human adipocytes

Effects of leptin on the differentiation and metabolism of human adipocytes

Metabolomics reveals an entanglement of fasting leptin concentrations with fatty acid oxidation and gluconeogenesis in healthy children

Serum Leptin Concentration In Children With Chronic Renal Failure

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