Lipoic acid stimulates bone formation in ovariectomized rats in a dose-dependent manner

Abstract

This study was undertaken to determine the osteotropic effect of different doses of lipoic acid (LA) on the mineralization of bone tissue in female Wistar rats with experimental osteopenia induced by bilateral ovariectomy. Fifty-six rats were randomly selected and submitted to either a sham operation (n = 8) or an ovariectomy (n = 48). The ovariectomized rats were randomly placed into two control groups, treated subcutaneously with either physiological saline or 17β-estradiol in the dose of 4 μg/kg body mass per day, and four experimental groups that received LA subcutaneously in the doses of 12.5, 25, 50, and 100 mg/kg body mass per day (n = 8 in each group). After 28 days of experimental treatment, the rats were sacrificed, and body mass, total skeletal density, and body composition were recorded. Blood serum and isolated femora were stored for further analysis. Our results revealed that the osteoprotective effect of LA was dose-dependent and was observed in rats treated with 50 and 100 mg/kg of LA. Moreover, the LA applied to the ovariectomized rats in the dose of 50 mg/kg not only stopped the bone resorption, but stimulated its formation.

Alpha-Lipoic Acid Alleviates High-Glucose Suppressed Osteogenic Differentiation of MC3T3-E1 Cells via Antioxidant Effect and PI3K/Akt Signaling Pathway

“…The potency of ALA for scavenging certain ROS and peroxynitrite and mitigating inflammation-induced bone loss has been demonstrated in several models [31]. Radzki et al [32] used an ovariectomized rat model showed that ALA applied to the ovariectomized rats in the dose of 50 mg/kg not only stopped the bone resorption, but stimulated its formation. Cell culture studies suggest that [33] media supplementation with ALA (0.1 – 1 mM) can protect osteoblasts from the damaging effect of extracellular ROS by restoring endogenous antioxidant defences and raised alkaline phosphatase activity, meanwhile up-regulated key genes involved in bone mineralization, i.e., collagen type-I and osteocalcin.…”

Influence of snail meat in the diet on mandibular bone loss in male rats: A densitometric, tomographic and morphometric study

“…Both trabecular and cortical compartments of bone tissue are very sensitive to any influences affecting bone metabolism. However, mostly the changes in trabecular bone tissue are more intensive (25,28). In our study, the tomographic analysis demonstrated a negative influence of each type of snail-based diet on both compartments of bone (cortical and trabecular).…”

“…It is possible that the consumption of snail meat as the sole source of protein causes effects similar to osteopenia or osteoporosis. The changes in the structure of the mandibles and their mechanical properties also resembled those in gonadectomized animals (24)(25)(26)28). In the last 20 years, a number of publications have demonstrated that the systemic bone mass loss that causes osteoporosis is highly correlated with the loss of teeth.…”

“…Dual X-ray absorptiometry (DXA) and peripheral quantitative tomography (pQCT) are the criterion standard for the evaluation and diagnosis of bone condition, They are very sensitive and reproducible techniques for measuring bone quality, not only in humans, but also in small animals (4,(25)(26)(27)(28). The quality of bone tissue is also measured by its resistance to fractures which occur when loads exceed the bone strength.…”

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

“…In addition, it must also be underlined that the Tot.vBMD and Tot.BMC in DFM results were also lower in the chromium treated rats, when compared to our control. It is also well-known that trabecular bone tissue is more sensitive (than cortical compartment) to any influences affecting bone metabolism, and the changes in trabecular bone tissue are more intensive [14,15].…”