Lipogenic gene expression in abdominal adipose and liver tissues of diet-induced overweight cats

Lee, Peter; Mori, Akira; Takemitsu, Hiroshi; Yamamoto, Ichiro; Arai, Toshiro

doi:10.1016/j.tvjl.2011.04.003

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…The conclusion of this study was that in an overweight cat model DNL does not occur in the liver, but it occurs in the visceral adipose tissue. 46 This supports the finding of Hall et al, 40 that the origin of the enhanced hepatic TGs in cats with FHL is the visceral adipose tissue and not the liver.…”

Section: Discussionsupporting

confidence: 85%

“…In a model of a diet-induced overweight state in cats, lipogenic gene expression ( SREBP-1c, FASN and ATP citrate lyase [ ACLY ]) in lipid sensitive tissue, that is liver, subcutaneous and abdominal adipose tissue, has been evaluated and compared with lean animals. 46 As already demonstrated in humans, a positive energy imbalance due to overnutrition and obesity can activate SREBP-1c and other secondary lipogenic gene expressions, enhancing lipogenesis in adipose and liver tissues contributing to liver steatosis. 18,19,21 In the overweight cats, mRNA expression of SREBP-1c and ATP citrate lyase were markedly decreased in liver tissue, whereas FASN expression remained similar to that of control cats.…”

Section: Discussionmentioning

confidence: 94%

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Gene expressions of de novo hepatic lipogenesis in feline hepatic lipidosis

Valtolina

Robben

Wolferen

et al. 2019

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to evaluate if de novo hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with feline hepatic lipidosis (FHL). Methods Lipogenic gene expression of peroxisome proliferator-activated receptor-alpha ( PPAR-α), peroxisome proliferator-activated receptor-gamma ( PPAR-γ), fatty acid synthase ( FASN) and sterol regulatory element-binding factor ( SREBF1) were evaluated using quantitative RT-PCR in liver tissue of six cats with FHL and compared with the liver tissue of eight healthy cats. Results In liver tissue, PPAR-α, PPAR-γ and FASN mRNA expression levels were not significantly different ( P >0.12, P >0.89 and P >0.5, respectively) in the FHL group compared with the control group. SREBF1 gene expression was downregulated around 10-fold in the FHL group vs the control group ( P = 0.039). Conclusions and relevance The downregulation of SREBF1 in the liver tissue of cats with FHL does not support the hypothesis that de novo lipogenesis in the liver is an important pathway of fatty acid accumulation in FHL.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 94%

Gene expressions of de novo hepatic lipogenesis in feline hepatic lipidosis

Valtolina

Robben

Wolferen

et al. 2019

Journal of Feline Medicine and Surgery

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“…In overweight cats, however, the expression of most of these genes was downregulated in the subcutaneous vs. omental adipose tissue, except for ACL. These data indicate that in an overweight state, subcutaneous adipose and hepatic tissues have a reduced capacity for lipid storage whereas omental adipose tissue continues to foster lipid deposition (Lee et al, 2011).…”

Section: Feline Studiesmentioning

confidence: 83%

“…Hepatic and subcutaneous adipose tissues of overweight cats also had less SREBP-1 mRNA transcription (0.29-to 0.71-and 0.30-to 0.77-fold change, respectively) and ATP citrate lyase (ACL) mRNA transcription (0.32-to 0.90-and 0.24-to 0.48-fold change, respectively). Transcription of FAS mRNA was also decreased in subcutaneous adipose tissue (0.0-to 0.79-fold change) vs. control whereas SREBP-1 (1.0-to 4.9-fold change) and FAS (1.2-to 7.6fold change) mRNA transcription was increased in the abdominal omental adipose tissue of overweight cats vs. control (Lee et al, 2011). Comparison of subcutaneous and omental adipose depots between lean and overweight cats showed that changes in gene expression of SREBP-1, FAS, and ACL may depend on depot.…”

Section: Feline Studiesmentioning

confidence: 89%

COMPANION ANIMALS SYMPOSIUM: Nutrigenomics: Using gene expression and molecular biology data to understand pet obesity1

Godoy

Swanson

2013

Journal of Animal Science

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Approximately 55% of dogs and 53% of cats in the United States are considered overweight or obese. The domestication of dogs and cats and, more recently, their anthropomorphism, have drastically changed their environment and social behavior. A greater manifestation of chronic diseases is observed with pet obesity (e.g., insulin resistance, type-2 diabetes, musculoskeletal disorders). The advances in "omics" technology may provide new tools to investigate the complexity of obesity and its comorbidities. The field of nutrigenomics focuses specifically on the mechanisms by which nutrients and dietary bioactive molecules affect gene expression. The main objective of this review is to discuss factors involved in the etiology of pet obesity and demonstrate how the field of nutrigenomics has been used to better understand and characterize this disease. Currently, most of the genomics literature available on companion animal obesity has focused on adipose tissue, with fewer studies focused on other tissues (e.g., skeletal muscle, liver). Initial studies focused on the sequence and functionality of a few specific genes, such as leptin and adiponectin, and identified their association with obesity. Subsequent studies focused on gene expression levels across tissues and how they were impacted by BW status or if animals were intact, spayed, or neutered. Dietary interventions to induce obesity, promote BW loss, or alter dietary nutrient profile have also been investigated. Diets including prebiotics, green tea extract, or increased concentrations of protein have been shown to modify the expression of several genes related to glucose and lipid metabolism in adipose [e.g., uncoupling protein-2, carnitine palmitoyltransferase-1, PPARα, lipoprotein lipase (LPL), and glucose transporter 4] and skeletal muscle (e.g., PPARα and LPL) tissues. In general, the outcomes derived from these studies demonstrated that dogs and cats share similar adipokines and hormones to other species, and they are affected in a similar fashion during obesity. They also indicate that gene transcription modifications may preclude clinical signs, which may become a useful tool in the management and prevention of obesity.

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“…5 Cats are more prone to become obese compared with dogs due to their unique characteristics in glucose and lipid metabolism such as a lack of hepatic glucokinase activities and low insulin signaling activities. 6,7 Cats are also prone to be insulin resistant compared with dogs due to low mRNA expression of insulin receptor substrate-1 (IRS-1), insulin receptor substrate-2 (IRS-2), and phosphatidylinositol 3-kinase (PI3-K) P-85 in insulin-responsive tissues (liver, skeletal muscle, abdominal fat, white blood cells). 8 In cats, the prevalence of obesity has increased with aging in recent decades as it has in humans.…”

Section: Introductionmentioning

confidence: 99%

<p>Effects of Age on Inflammatory Profiles and Nutrition/Energy Metabolism in Domestic Cats</p>

Mizorogi¹,

Kobayashi²,

Ohara³

et al. 2020

VMRR

Self Cite

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Background: Animals tend to increase in body weight and body condition score (BCS) with aging. Serum diagnostic markers related to energy metabolism may show changes even in healthy cats with aging. Materials and Methods: Seventy domestic cats were recruited for this study. Based upon the modified AAFP-AAHA Feline Life Stage Guidelines, animals were divided into six groups: Junior (7 months-2 years), Prime (3 −6 years), Mature (7-10 years), Senior (11-14 years), Geriatric-obese (15 years ≤) and Geriatric-thin (15 years ≤). Their body condition scores (BCS) ranged from 3/9 to 9/9. Changes in metabolites, inflammatory markers, hormone concentrations and enzyme activities related to energy metabolism were investigated in serum of 70 domestic cats of various ages. Results: Serum glucose (GLU) concentrations in the Mature, Senior, and Geriatric-obese groups were significantly higher than those in the Junior group. Serum amyloid A (SAA) concentrations in the Geriatric-thin group were significantly increased compared with the Junior group. SAA concentrations in the Geriatric-obese group tended to increase although there were no statistically significant differences. In the Mature, Senior, Geriatricobese and Geriatric-thin groups, malate dehydrogenase/lactate dehydrogenase (M/L) ratio, an energy metabolic indicator, tended to decrease compared with the Junior group. In the Senior group, triglyceride (TG) concentrations were significantly increased compared with the Junior group. In the Geriatric-obese and Geriatric-thin groups, blood urea nitrogen (BUN) concentrations were significantly increased compared with the Junior group. In the Geriatric-obese group, albumin (ALB) concentrations were decreased compared with the Junior group. Conclusion: Aged domestic cats tend to increase in body weight and BCS. In addition, serum GLU, TG, SAA, and BUN concentrations increased and serum ALB concentrations and M/L ratio decreased. These diagnostic markers may be useful to detect small changes related to energy metabolism with aging that may cause obesity with light inflammation in healthy cats.

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Lipogenic gene expression in abdominal adipose and liver tissues of diet-induced overweight cats

Cited by 6 publications

References 21 publications

Gene expressions of de novo hepatic lipogenesis in feline hepatic lipidosis

Gene expressions of de novo hepatic lipogenesis in feline hepatic lipidosis

COMPANION ANIMALS SYMPOSIUM: Nutrigenomics: Using gene expression and molecular biology data to understand pet obesity1

<p>Effects of Age on Inflammatory Profiles and Nutrition/Energy Metabolism in Domestic Cats</p>

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