Lipids, Fatty Acids and Sterols of Cystoseira abies - marina

Moreno, Paulo Roberto Hrihorowitsch; Petkov, Georgi V.; Ramazanov, Zakir; Garsia, G.

doi:10.1515/botm.1998.41.1-6.375

Cited by 13 publications

(11 citation statements)

References 9 publications

(3 reference statements)

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“…Total carbohydrates were quantified as glucose equivalents by the phenol-sulfuric acid method (Dubois et al 1956). Lipid content was estimated gravimetrically after reflux extraction with ethanol for 30 min x3 and reextraction with chloroform (Moreno et al 1998). The experiments were done in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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RESPONSE OF TRACHYDISCUS MINUTUS (XANTHOPHYCEAE) TO TEMPERATURE AND LIGHT¹

Gigova

Ivanova

Gacheva

et al. 2011

Journal of Phycology

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The effects of different temperatures and light intensities on growth, pigments, sugars, lipids, and proteins, as well as on some antioxidant and proteolytic enzymes of Trachydiscus minutus (Bourr.) H. Ettl, were investigated. The optimum growth temperature and light intensity were 25°C and 2 × 132 μmol photons · m(-2 ) · s(-1) , respectively. Under these conditions, proteins were the main biomass components (33.45% dry weight [dwt]), with high levels of carbohydrates (29% dwt) and lipids (21.77% dwt). T. minutus tolerated temperatures between 20°C and 32°C, with only moderate changes in cell growth and biochemical composition. Extremely low (15°C) and high (40°C) temperatures decreased chl and RUBISCO contents and inhibited cell growth. The biochemical response of the alga to both unfavorable conditions was an increase in lipid content (up to 35.19% dwt) and a decrease in carbohydrates (down to 13.64% dwt) with much less of a change in total protein content (in the range of 30.51%-38.13% dwt). At the same time, the defense system of T. minutus was regulated differently in response to heat or cold treatments. Generally, at 40°C, the activities of superoxide dismutase (SOD), catalase (CAT), and proteases were drastically elevated, and three polypeptides were overexpressed, whereas the glutathione reductase (GR) and peroxidase (POD) activities were reduced. In contrast, at 15°C, all these enzymes except GR were suppressed. The effect of light was to enhance or decrease the temperature stress responses, depending on intensity. Our studies demonstrate the broad temperature adaptability of T. minutus as well as the potential for the production of valuable algal biomass.

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Section: Methodsmentioning

confidence: 99%

“…1956). Lipid content was estimated gravimetrically after reflux extraction with ethanol for 30 min x3 and reextraction with chloroform (Moreno et al. 1998).…”

mentioning

confidence: 99%

RESPONSE OF TRACHYDISCUS MINUTUS (XANTHOPHYCEAE) TO TEMPERATURE AND LIGHT¹

Gigova

Ivanova

Gacheva

et al. 2011

Journal of Phycology

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“…Lipid content was estimated gravimetrically after reflux extraction with ethanol for 30 min (three times) and re‐extraction with chloroform (Moreno et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…Total protein content was assessed by the method of Lowry, with BSA as a standard. Lipid content was estimated gravimetrically after reflux extraction with ethanol for 30 min (three times) and re-extraction with chloroform (Moreno et al 1998).…”

Section: Methodsmentioning

confidence: 99%

Growth, biochemical and enzymatic responses of thermal cyanobacterium Gloeocapsa sp. (Cyanophyceae) to temperature and irradiance

Gacheva

Gigova

Ivanova

et al. 2013

Phycological Research

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Summary The present study describes a strain of Gloeocapsa sp. designated as Gacheva 2007/R‐06/1, originally isolated from a geothermal flow located in Rupite, Bulgaria. To evaluate whether this cyanobacterium is locally adapted to hot environment or has the ability to tolerate lower temperatures, its growth, biochemical composition, enzyme isoforms and activity of the main antioxidant enzymes and proteases were characterized under various temperatures and two irradiance levels. The strain was able to grow over the whole temperature range (15–40°C) under two different photon fluence densities – 132 μmol photons m−2 s−1 (unilateral, low light, LL) and 2 × 132 μmol photons m−2 s−1 (bilateral, high light, HL). The best growth occurred at either 34°C and LL or at 36°C and HL, but significant growth inhibition was noted at 15°C and 40°C. Low temperature treatment (15°C) resulted in higher levels of total protein and an increased activity of manganese superoxide dismutase (MnSOD) and glutathione reductase, as compared to optimum growth temperatures. After simultaneous exposure to 15°C and HL, increases in lipid content and activity of iron superoxide dismutase and catalase (CAT) were also observed. Cultivation of cells at 40°C enhanced MnSOD, CAT and peroxidase activities, regardless of irradiance level. Increased total protein content and protease activity at 40°C was only associated with the HL treatment. Overall, these results indicate that Gloeocapsa sp. strain Gacheva 2007/R‐06/1 used different strategies to enable cells to efficiently acclimate and withstand adverse low or high temperatures. This strain obviously tolerates a wide range of temperatures below its natural habitat temperature, and does not seem to be locally adapted to its original thermal regime. It behaved as a thermotolerant rather than a thermophilic cyanobacterium, which suggests its wider distribution in nature.

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“…HPLC-HPLC and capillary gas chromatography (GC) with mass spectrometry detection through chemical ionization has been described for the determination of sterol esters from green algae [12]. Red, green and brown algae were investigated for their cholesterol content by GC [8,13,14].…”

Section: Introductionmentioning

confidence: 99%