Determination of total sterols in brown algae by Fourier transform infrared spectroscopy

Bouzidi, Naïma; Daghbouche, Yasmina; Hattab, Mohamed El; Aliche, Zahia; Culioli, Gérald; Piovetti, Louis; Garrigues, Salvador; Guárdia, Miguel de la

doi:10.1016/j.aca.2008.04.028

Cited by 37 publications

(20 citation statements)

References 15 publications

(19 reference statements)

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“…Fucosterol, the most abundant sterol in brown algae,[20] has been shown to be antioxidant,[21] antidiabetic,[22] antifungal,[23] and cytotoxic. [6] In our previous study,[24] hexane fractions of Sargassum swartzii and Cystoseira myrica showed cytotoxicity against Caco-2 and T47D cell lines, respectively and they were observed for increasing apoptosis in Caco-2 and T47D cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line

Khanavi¹,

Gheidarloo²,

Sadati³

et al. 2012

Phcog Mag

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Context:Marine algae produce different secondary metabolites with a wide range of biological activities. Many studies have been achieved on the screening of biological effects of marine organisms and a lot of active compounds were isolated and characterized.Aims:In an attempt to find cytotoxic compound of hexane fraction, isolation, identification, and cytotoxicity of active compound of this fraction were performed.Materials and Methods:In this study, total methanolic (70%) extract and partition fractions of hexane, chloroform (CHCl3), ethyl acetate (EtOAc), and MeOH–H2O of Sargassum angustifolium, Chondria dasyphylla, and Ulva flexuosa, collected from coastlines of the Persian Gulf in south of Iran, were studied against colon carcinoma (HT-29), colorectal adenocarcinoma (Caco-2), breast ductal carcinoma (T47D), and Swiss mouse embryo fibroblast (NIH 3T3) cell lines by MTT assay.Statistical Analysis Used:IC50 (median growth inhibitory concentration) values were calculated by Sigmaplot (10) software.Results:Hexane fraction of Chondria dasyphylla (IC50 82.26 ± 4.09 μg/ml) and MeOH-H2O fraction of Ulva flexuosa (IC50 116.92 ± 8.58 μg/ml) showed cytotoxic activity against proliferation of T47D cells. Hexane fraction of Sargassum angustifolium was also observed for cytotoxicity against T47D and HT-29 cell lines (IC50 166.42 ± 26.7 and 190.24 ± 52.8 μg/ml), respectively. An investigation of a component from the hexane fraction of Sargassum angustifolium yielded a steroidal metabolite, fucosterol, with cytotoxicity in T47D and HT29 (IC50 27.94 ± 9.3 and 70.41 ± 7.5 μg/ml).Conclusions:These results indicated that fucosterol, the most abundant phytosterol in brown algae, is responsible for cytotoxic effect of this extract against breast and colon carcinoma cell lines.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line

Khanavi¹,

Gheidarloo²,

Sadati³

et al. 2012

Phcog Mag

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“…The sterols exhibited characteristic vibration bands ( Fig. 2) between 2860 and 2930 cm À1 [32][33][34]. The C-OH stretching vibration for CH and PS in sterols derived from the OH group at the 3b-position and at a 5D-double bond were observed at $1050 cm -1 .…”

Section: Effect On Crystal Structurementioning

confidence: 97%

Templated cocrystallization of cholesterol and phytosterols from microemulsions

Rozner

Popov

Uvarov

et al. 2009

Journal of Crystal Growth

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“…Seaweeds having a very important role in the marine environment have been studied because they are abundant and easily accessible. Different biological activities of extracts have been reported and approved by several works [6,9,11,27].…”

Section: Chlorophyceae (Green Algae)mentioning

confidence: 99%

“…Marine algae have been widely used as biological material in Moroccan laboratories [6,7] and were the source of many biological molecules [8][9][10][11]. However, the chemistry of marine algae of the Moroccan coast is still undefined.…”

Section: Introductionmentioning

confidence: 99%

Screening of antimicrobial activity of macroalgae extracts from the Moroccan Atlantic coast

Wahidi

Amraoui

et al. 2015

Annales Pharmaceutiques Françaises

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Determination of total sterols in brown algae by Fourier transform infrared spectroscopy

Cited by 37 publications

References 15 publications

Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line

Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line

Templated cocrystallization of cholesterol and phytosterols from microemulsions

Screening of antimicrobial activity of macroalgae extracts from the Moroccan Atlantic coast

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