Lipidomics study of the protective effects of isosteviol sodium on stroke rats using ultra high-performance supercritical fluid chromatography coupling with ion-trap and time-of-flight tandem mass spectrometry

Yang, Yang; Zhong, Qisheng; Zhang, Hao; Mo, Canlong; Yao, Jing; Huang, Taohong; Zhou, Ting; Tan, Wen

doi:10.1016/j.jpba.2018.05.022

Cited by 24 publications

(16 citation statements)

References 35 publications

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“…The circles represent biomarkers which were not significantly changed after PD treatment. (Yang Y. et al, 2018). Therefore, the interference of PD with PC may change the ability of the body to resist bacteria.…”

Section: Change Of Glycerophospholipid Metabolismmentioning

confidence: 99%

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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Ethnopharmacological Relevance: Diarrhea is a major medical problem in clinical practice. According to the theory of traditional Chinese medicine (TCM), different types of diarrhea should be treated with different TCM formulations based on the targeted medical condition. Dampness-heat diarrhea (DHD) is a serious diarrheal disease and Pulsatilla decoction (PD), a TCM, has been found effective against DHD. Objective: The aim of this study was to clarify the mechanism of action of PD in DHD using an untargeted lipidomics strategy. Materials and Methods: Wistar rats were randomized to four groups, including the control group, model group, PD groups and self-healing group. The PD groups were given a daily intragastric gavage of PD at doses of 3.76 g/kg. The rat model of DHD established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment, drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of DHD. Then the clinical symptoms and signs, blood routine, serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed to evaluate DHD model and therapeutic effect of PD. Lipid biomarkers of DHD were selected by comparing the control and model groups with the colon lipidomics technology and an ultra-high performance liquid chromatography (UHPLC) coupled with Q Exactive plus mass analyzer. Multivariate statistical analysis and pattern recognition were employed to examine different lipids within the colon of PD-treated rats. Results: The clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of DHD. After treatment with PD, the clinical symptoms and signs of the rats with DHD were improved; the indexes of blood routine and inflammatory cytokines levels tended to be normal. The lipidomics profile of the model group were

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Section: Change Of Glycerophospholipid Metabolismmentioning

confidence: 99%

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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“…Furthermore, 24 lipid classes including 436 lipid species were identified in porcine brain extract using the same method. Yang et al [63] carried out untargeted lipidomics research based on the UHPSFC coupling with ion-trap and time-of-flight tandem mass spectrometry (UHPSFC -IT-TOF/MS) to investigate the lipid profiles of stroke rats with STV-Na treatment. This UHPSFC -IT-TOF/MS-based method achieved a fast separation of various lipids within 9 min with a qualified repeatability.…”

Section: Untargeted Lipid Profiling By Sfcmentioning

confidence: 99%

Advances of supercritical fluid chromatography in lipid profiling

Yang

Liang

Yang

et al. 2019

Journal of Pharmaceutical Analysis

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Supercritical fluid chromatography (SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development.

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“…Animals were maintained on a 12 h light/dark cycle with free access to a standard diet and water. This study used the same samples of rats collected previously and the model evaluations were described in our previous work (Yang et al, 2018). The focal MCAO model was based on the method of Longa et al, as previously described (Longa, Weinstein, Carlson, & Cummins, 1989).…”

Section: Experimental Animals and Treatment Protocolmentioning

confidence: 99%

“…The model building was successfully verified by three aspects: the monitoring of regional cerebral blood flow (rCBF); the neurobehavioral abnormality evaluation; and the 2,3,5-triphenyltetrazolium chloride staining of brain slices. This study used the same samples of rats collected previously and the model evaluations were described in our previous work (Yang et al, 2018). Animal welfare and experiments were performed in accordance with the relevant laws and institutional guidelines of the Institutional Animal Care and Use Committee of Guangdong Pharmaceutical University.…”

Section: Experimental Animals and Treatment Protocolmentioning

confidence: 99%

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Yang

Zhang

et al. 2018

Biomedical Chromatography

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Cerebral ischemia remains a major cause of mortality and a long-term disability with limited therapies. Isosteviol sodium (STV-Na) was proved to exert significant protective effects on cerebral ischemia, but the protective mechanism was not understood. In this study, the protective effects of STV-Na on cerebral ischemia were investigated by the metabolomics approach based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry technique. The models of ischemic rats were established and the brain tissues were employed for metabolomics analyses. The principal component analysis showed that the model group was clearly separated from the sham group, while both STV-Na and edaravone groups were located between the sham and the model groups, which indicated that STV-Na as well as edaravone had protective effects on cerebral ischemia. Eighteen differential metabolites which had significant differences between the sham and the model groups were screened and identified. After the administration of STV-Na, all 18 differential metabolites were regulated to the levels between the sham and the model groups, and 12 of them presented significant differences between the model and STV-Na groups. The pathway analysis indicated that the protective effects of STV-Na on cerebral ischemia might be associated with the regulation of several metabolic pathways, i.e. glycerophospholipid metabolism, arachidonic acid metabolism and linoleic acid metabolism.

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Lipidomics study of the protective effects of isosteviol sodium on stroke rats using ultra high-performance supercritical fluid chromatography coupling with ion-trap and time-of-flight tandem mass spectrometry

Cited by 24 publications

References 35 publications

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Advances of supercritical fluid chromatography in lipid profiling

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

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