Advances of supercritical fluid chromatography in lipid profiling

Yang, Yang; Liang, Yanshan; Yang, Joseph; Ye, Fengying; Zhou, Ting; Li, Gongke

doi:10.1016/j.jpha.2018.11.003

Cited by 39 publications

(12 citation statements)

References 69 publications

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“…Due to high solubility of lipids in supercritical CO 2 , which has similar solvent strength as hexane, SFC is used more often in the separation of different classes of lipids in the field of lipidomics. 21 Here, we look at the SFC separation of cholesterol, one of the most known lipids, and cholestanol, which has a similar structure to cholesterol, differing in only one double bond ( Figure 8 ). For the separation of these compounds, a 1 μL mixture of 28 mM cholestanol and 43 mM cholesterol in chloroform was injected into the SFC.…”

Section: Resultsmentioning

confidence: 99%

An Optimized NMR Stripline for Sensitive Supercritical Fluid Chromatography-Nuclear Magnetic Resonance of Microliter Sample Volumes

et al. 2020

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To optimize sensitivity, there has been an increasing interest in the miniaturization of NMR detectors. In our lab, a stripline NMR detector has been developed, which provides high resolution and is scalable to a large range of sample volumes. These features make it an ideal detector for hyphenated techniques. In this manuscript, we demonstrate a stripline probe, which is designed for combining supercritical fluid chromatography (SFC) experiments with NMR. It features a novel stripline chip, designed to reduce the signal from the contact pads, which results in an improved lineshape. An external lock circuit provides stability over time to perform signal averaging or multidimensional experiments. As proof of concept, we demonstrate the SFC-NMR technique with this stripline probe using a mixture of cholesterol and cholestanol, which is relevant for studying cerebrotendinous xanthomatosis. Additionally, this probe makes it possible to record high-resolution spectra of samples with a high spin density. This means that it is possible to directly observe shifts due to the nuclear demagnetizing field in the “homomolecular” case, which is challenging using conventional probes due to broadening effects from radiation damping.

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Section: Resultsmentioning

confidence: 99%

An Optimized NMR Stripline for Sensitive Supercritical Fluid Chromatography-Nuclear Magnetic Resonance of Microliter Sample Volumes

et al. 2020

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“…The SPE−SFC system consists of an SPE module and an SFC module. The configuration of the SPE−SFC system is quite similar to that of the common online SPE−UHPLC system because both of them use the valve switching strategy [105]. SFE−SFC−MS or SFE−SFC−MS/MS could become a fundamental tool for comprehensive lipid profiling [53, 88].…”

Section: Discussionmentioning

confidence: 99%

Supercritical fluid chromatography hyphenated to mass spectrometry for metabolomics applications

Gordillo

2020

J of Separation Science

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While supercritical fluid chromatography was developed over 50 years ago, it is only over the past 15 to 20 years that it has become routinely utilized. Along with the commercialization of a new generation of instruments, during the last 20 years supercritical fluid chromatography has improved performance, reliability, and robustness. Supercritical fluid chromatography is fully compatible with mass spectrometric techniques. This review compiles the application of supercritical fluid chromatography separations coupled to mass spectrometry instrumentation for the exploration, profiling, and quantitation of metabolites during the last two decades. The selection of metabolites chosen for this article have direct applications in preclinical models of disease and clinical applications as potential biomarkers of disease including lipids, steroid hormones, bile acids, polar metabolites, peptides, and proteins.

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“…Several studies have reported the application of SFC to the rapid analysis of lipid composition. For example, it has been applied to the analysis of phospholipids and TAG, and to lipids including TAG regioisomer (Lee et al, 2014; Takeda et al, 2018; Yang et al, 2019). SFC has also been used for the enantiomeric analysis of chemical substances used as pharmaceutical ingredients (Klerck et al, 2012, 2014).…”

Section: Introductionmentioning

confidence: 99%

A Practical Method for Analysis of Triacylglycerol Isomers Using Supercritical Fluid Chromatography

Masuda

Abe²,

Murano

2020

J Americ Oil Chem Soc

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Triacylglycerol (TAG) isomers have been reported to have differing physical and nutritional properties. The analysis of TAG isomers is therefore important for understanding the physical properties of lipids as well as their digestion and absorption. However, methods for the quantitative analysis of TAG regioisomers and enantiomers in vegetable oils and biological samples are still under development. Recently, methods using recycle high‐performance liquid chromatography (HPLC) and silver ion column‐HPLC have been reported. However the recycle HPLC method requires more than 1 hour, in general, for each sample that is analyzed. Furthermore, existing methods are unable to quantify regioisomers and enantiomers simultaneously. Thus, we aimed to develop a practical method to simultaneously quantify regioisomers and enantiomers of TAG. Three isomers of sn‐POO, OPO, and OOP were separated by supercritical fluid chromatography coupled with triple quadrupole mass spectrometer (SFC/MS/MS) using a CHIRALPAK® IG‐U column with acetonitrile and methanol as mobile phase. The separation was completed in 40 min, which is a shorter run time than the conventional techniques published to date. Linear calibration curves with standards were obtained and used to quantify sn‐OPO, sn‐POO, and sn‐OOP in extra virgin olive oil, refined olive oil, palm oil, palm olein, and interesterified palm olein.

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Advances of supercritical fluid chromatography in lipid profiling

Cited by 39 publications

References 69 publications

An Optimized NMR Stripline for Sensitive Supercritical Fluid Chromatography-Nuclear Magnetic Resonance of Microliter Sample Volumes

An Optimized NMR Stripline for Sensitive Supercritical Fluid Chromatography-Nuclear Magnetic Resonance of Microliter Sample Volumes

Supercritical fluid chromatography hyphenated to mass spectrometry for metabolomics applications

A Practical Method for Analysis of Triacylglycerol Isomers Using Supercritical Fluid Chromatography

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