Lipidomic Profiling Reveals the Reducing Lipid Accumulation Effect of Dietary Taurine in Groupers (Epinephelus coioides)

Abstract

A lipidomic analysis was conducted to provide the first detailed overview of lipid molecule profiles in response to dietary lipid and taurine and associations of liver lipid-lowering effects of dietary taurine with lipid molecular species and the positional distributions of fatty acids in the liver of juvenile orange-spotted groupers (Epinephelus coioides). The results indicated that the liver was more sensitive to varied dietary lipid and taurine contents than the muscle with regard to lipid molecules. A total of 131 differential lipid molecules (DLMs) were observed in the liver of groupers when dietary taurine was increased from 0 to 1% at 15% lipid, among which all the up and down-regulated DLMs are phospholipids (PLs) and triglycerides (TGs), respectively. The liver content of TGs containing 18:2n-6 attached at the sn-2 and sn-3 positions on the glycerol backbone increased with increasing dietary lipid from 10 to 15% but decreased with increasing dietary taurine from 0 to 1%. Therefore, dietary taurine can not only reduce lipid accumulation through decreasing the contents of TGs containing 18:2n-6 at the sn-2 and sn-3 positions but also enhance the anti-inflammatory capacity and health status of groupers. This study will also provide a new insight into the function of taurine in farmed fish.

Hepatic Transcriptome Analysis Provides New Insight into the Lipid-Reducing Effect of Dietary Taurine in High–Fat Fed Groupers (Epinephelus coioides)

“…However, the underlying mechanism involved in the regulation of lipid metabolism still remains unclear. Recent studies have shown that dietary taurine supplementation in 15% fat feed could reduce lipid accumulation through reducing the contents of triglyceride molecules containing 18:2n–6 at the sn – 2 and sn – 3 positions [ 25 ] and through accelerating lipid absorption of taurine−conjugated bile acids and fatty acid β-oxidation, and inhibiting lipogenesis [ 26 ]. However, determining how dietary taurine modulates lipid metabolism of fish at the transcriptional level has not yet been investigated.…”

Lipidomics analysis reveals new insights into crisp grass carp associated with meat texture

“…Other fish species displayed a comparable result, although the occurrence rate of these findings varied. In Nile tilapia ( Oreochromis niloticus ) and orange-spotted grouper ( Epinephelus coioides ) muscular tissues, TG emerged as the dominant lipid class, with PC and PE following closely behind [ 23 , 24 ]. Given that TG primarily functions as a source of energy [ 25 ], PC and PE play crucial roles in the proper integration of membrane proteins and membrane fusion and fission processes [ 24 , 26 ].…”

Replacement of Dietary Fishmeal with Clostridium autoethanogenum Protein on Lipidomics and Lipid Metabolism in Muscle of Pearl Gentian Grouper

“…MUFA, especially 18 : 1n−9, is a "healthy" fatty acid for human body, which can remove bad cholesterol and protect cardiovascular and cerebrovascular health, etc. [42]. The concentrations of MUFA were 361.46 μg/g, dominated by 18 : 1n−9c (146.34 μg/g).…”