Lipidomic profiling of extracellular vesicles derived from prostate and prostate cancer cell lines

Brzozowski, Joshua S.; Jankowski, Helen; Bond, Danielle R.; McCague, Siobhan B.; Munro, Benjamin R.; Predebon, Melanie J.; Scarlett, Christopher J.; Skelding, Kathryn A.; Weidenhofer, Judith

doi:10.1186/s12944-018-0854-x

Cited by 117 publications

(131 citation statements)

References 49 publications

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“…These differences in lipid composition could stem from the presence of lipoproteins in Lipo‐NPs, as triglycerides are associated with lipoproteins in the body 36. However, other studies have shown that cancer cell‐derived EVs contain high amounts of glycerolipids, making it difficult to draw any conclusions 37…”

Section: Resultsmentioning

confidence: 98%

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Tian

Ticer

Wang

et al. 2020

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Extracellular vesicles secreted from adipose‐derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti‐inflammatory properties of adipose tissue‐derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC‐EVs, lipoaspirate nanoparticles (Lipo‐NPs) take less time to process (hours compared to months) and cost less to produce (clinical‐grade cell culture facilities are not required). The physicochemical characteristics and anti‐inflammatory properties of Lipo‐NPs are evaluated and compared to those of patient‐matched ADSC‐EVs. Moreover, guanabenz loading in Lipo‐NPs is evaluated for enhanced anti‐inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo‐NPs compared to ADSC‐EVs. Additionally, the uptake of Lipo‐NPs in hepatocytes and macrophages is higher. Lipo‐NPs and ADSC‐EVs have comparable protective and anti‐inflammatory effects. Specifically, Lipo‐NPs reduce toll‐like receptor 4‐induced secretion of inflammatory cytokines in macrophages. Guanabenz‐loaded Lipo‐NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.

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Section: Resultsmentioning

confidence: 98%

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Tian

Ticer

Wang

et al. 2020

Small

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“…A study on prostate cancer has shown that the accumulation of cholesterol esters in prostate cancer cells is associated with tumor progression and metastasis. This was confirmed by studies, using synthetic exosomes carried out by the Lombardo Group, which showed that exosomal lipids can increase the tumor aggressiveness, metastatic progression and drug resistance of pancreatic cancer cells [31].…”

Section: Tumor Progressionmentioning

confidence: 68%

Exosomes in Prostate Cancer Diagnosis, Prognosis and Therapy

Lorenc

Klimczyk

Michalczewska

et al. 2020

IJMS

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Prostate cancer (PCa) is the second most common cause of cancer-related mortality among men in the developed world. Conventional anti-PCa therapies are not effective for patients with advanced and/or metastatic disease. In most cases, cancer therapies fail due to an incomplete depletion of tumor cells, resulting in tumor relapse. Exosomes are involved in tumor progression, promoting the angiogenesis and migration of tumor cells during metastasis. These structures contribute to the dissemination of pathogenic agents through interaction with recipient cells. Exosomes may deliver molecules that are able to induce the transdifferentiation process, known as "epithelial to mesenchymal transition". The composition of exosomes and the associated possibilities of interacting with cells make exosomes multifaceted regulators of cancer development. Extracellular vesicles have biophysical properties, such as stability, biocompatibility, permeability, low toxicity and low immunogenicity, which are key for the successful development of an innovative drug delivery system. They have an enhanced circulation stability and bio-barrier permeation ability, and they can therefore be used as effective chemotherapeutic carriers to improve the regulation of target tissues and organs. Exosomes have the capacity to deliver different types of cargo and to target specific cells. Chemotherapeutics, natural products and RNA have been encapsulated for the treatment of prostate cancers.

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“…One potential application of TFAC utilizing ultrathin membranes as a microfluidic‐based technique would be isolation of EVs. Studies have indicated that not only the RNA content of these vesicles but also their protein varies by cell of origin as well as by the pathologic state of these cells . This differential cell state specific and cellular origin‐based content indicates that EVs could serve as biomarkers of disease.…”

Section: Discussionmentioning

confidence: 99%

Tangential Flow Microfluidics for the Capture and Release of Nanoparticles and Extracellular Vesicles on Conventional and Ultrathin Membranes

Dehghani

Lucas

Flax

et al. 2019

Adv Materials Technologies

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Membranes have been used extensively for the purification and separation of biological species. A persistent challenge is the purification of species from concentrated feed solutions such as extracellular vesicles (EVs) from biological fluids. Investigated is a new method to isolate micro‐ and nanoscale species termed tangential flow for analyte capture (TFAC), which is an extension of traditional tangential flow filtration. Initially, EV purification from plasma on ultrathin nanomembranes is compared between both normal flow filtration (NFF) and TFAC. NFF results in rapid formation of a protein cake which completely obscures any captured EVs and also prevents further transport across the membrane. On the other hand, TFAC shows capture of CD63 positive small EVs with minimal contamination. The use of TFAC to capture target species over membrane pores, wash, and then release in a physical process that does not rely upon affinity or chemical interactions is explored. This process is studied with model particles on both ultrathin and conventional thickness membranes. Successful capture and release of model particles is observed using both membranes. Ultrathin nanomembranes show higher efficiency of capture and release with significantly lower pressures indicating that ultrathin nanomembranes are well‐suited for TFAC of delicate nanoscale particles such as EVs.

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Lipidomic profiling of extracellular vesicles derived from prostate and prostate cancer cell lines

Cited by 117 publications

References 49 publications

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Exosomes in Prostate Cancer Diagnosis, Prognosis and Therapy

Tangential Flow Microfluidics for the Capture and Release of Nanoparticles and Extracellular Vesicles on Conventional and Ultrathin Membranes

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