Lipidomic and transcriptomic analysis reveals the self-regulation mechanism of Schizochytrium sp. in response to temperature stresses

Hu, Xuechao; Luo, Yangyang; Man, Yanli; Tang, Xiuyang; Bi, Zhi-Qian; Ren, Lu‐Jing

doi:10.1016/j.algal.2022.102664

Cited by 9 publications

(6 citation statements)

References 42 publications

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“…Therefore, most phospholipids and their derivatives are signaling molecules that can modulate cell behavior. [ 25,26 ] In this study, the significant changes in the relative content of most lipid subclasses in GPs under the treatment of ShV0 may be caused by the response of phospholipid molecules to the environment of cobalamin deficiency in cells. PC is the major phospholipid class in cell membranes and also plays a regulatory role in signaling.…”

Section: Resultsmentioning

confidence: 89%

Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp

Yang,

Liu,

Chen

et al. 2024

Biotechnology Journal

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Schizochytrium sp. is a heterotrophic microorganism capable of accumulating polyunsaturated fatty acids and has achieved industrial production of docosahexaenoic acid (DHA). It also has the potential for eicosapentaenoic acid (EPA) production. In this study, it was found that the cell growth, lipid synthesis and fatty acid composition of Schizochytrium sp. were significantly affected by the level of cobalamin in the medium, especially with regard to the content of EPA in the fatty acids. The content of EPA in the fatty acids increased 17.91 times, reaching 12.00%, but cell growth and lipid synthesis were significantly inhibited under cobalamin deficiency. The response mechanism for this phenomenon was revealed through combined lipidomic and transcriptomic analysis. Although cell growth was inhibited under cobalamin deficiency, the genes encoding key enzymes in central carbon metabolism were still up‐regulated to provide precursors (Acetyl‐CoA) and reducing power (NADPH) for the synthesis and accumulation of fatty acids. Moreover, the main lipid subclasses observed during cobalamin deficiency were glycerolipids (including glycerophospholipids), with EPA primarily distributed in them. The genes involved in the biosynthesis of these lipid subclasses were significantly up‐regulated, such as the key enzymes in the Kennedy pathway for the synthesis of triglycerides. Thus, this study provided insights into the specific response of Schizochytrium sp. to cobalamin deficiency and identified a subset of new genes that can be engineered for modification.

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Section: Resultsmentioning

confidence: 89%

Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp

Yang,

Liu,

Chen

et al. 2024

Biotechnology Journal

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“…Genes involved in triglyceride biosynthesis, fatty acid degradation, and β-oxidation have been reported to be correlated with lipid metabolism stages ( 39 ). Most of the lipids in Schizochytrium cells are stored in lipid droplets in the form of triglycerides (TAG) ( 40 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis Reveals an Eicosapentaenoic Acid Accumulation Mechanism in a Schizochytrium sp. Mutant

Feng

et al. 2023

Microbiol Spectr

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“…( Sun et al, 2018 ). Temperature is an important factor, it affects both cell growth and fatty acid profile in microorganisms ( Hu et al, 2022 ). Low-temperature conditions triggered the overexpression of ACC gene in the DHA accumulator Aurantiochytrium, which led to an increase in the accumulation of malonyl-Co A for subsequent fatty acid synthesis ( Ma et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of malate-induced Schizochytrium sp. FJU-512 reveals a novel pathway for biosynthesis of docosahexaenoic acid with enhanced expression of genes responsible for acetyl-CoA and NADPH accumulation

Zhang

Gao

et al. 2022

Front. Microbiol.

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Schizochytrium is one of the few oleaginous microalgae that produce docosahexaenoic acid (DHA)-rich lipids. In this study, global changes in gene expression levels of Schizochytrium sp. FJU-512 cultured with malate in a 15 l-bioreactor was analyzed using comparative transcriptomics. The changes were found mainly in the genes involved in oxidative phosphorylation, β-oxidation, and pentose phosphate pathways. Consequently, the global changes in genes associated with the pathways could lead to an increase in the influx throughputs of pyruvate, branched-chain amino acids, fatty acids, and vitamin B6. Our transcriptome analysis indicated pyruvate dehydrogenase E2 component and acetolactate synthase I/II/III large subunit as major contributors to acetyl-CoA biosynthesis, whereas glucose-6-phosphate dehydrogenase was indicated as the major contributor to the biosynthesis of NADPH. An increase in DHA titer of up to 22% was achieved with the addition of malate to the fed-batch culture of Schizochytrium sp. FJU-512. This study provides an alternate method to enhance DHA production in Schizochytrium sp. FJU-512 through malate induced upregulation of genes responsible for acetyl-CoA and NADPH biosynthesis.

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Lipidomic and transcriptomic analysis reveals the self-regulation mechanism of Schizochytrium sp. in response to temperature stresses

Cited by 9 publications

References 42 publications

Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp

Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp

Transcriptome Analysis Reveals an Eicosapentaenoic Acid Accumulation Mechanism in a Schizochytrium sp. Mutant

Transcriptome analysis of malate-induced Schizochytrium sp. FJU-512 reveals a novel pathway for biosynthesis of docosahexaenoic acid with enhanced expression of genes responsible for acetyl-CoA and NADPH accumulation

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