Transcriptome Analysis Reveals an Eicosapentaenoic Acid Accumulation Mechanism in a
            <i>Schizochytrium</i>
            sp. Mutant

Ou, Ying; Li, Yaqi; Feng, Shoushuai; Wang, Qiong; Yang, Hailin

doi:10.1128/spectrum.00130-23

Cited by 4 publications

(1 citation statement)

References 53 publications

(55 reference statements)

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“…Offline data were supported by online backscatter and respiratory data. The investigated thraustochytrid strain is known to form cell clusters, especially at higher cell densities (Ou et al 2023). This behaviour leads to inaccurate growth determination via optical density measurements.…”

Section: Growth Monitoring By Backscatter Light In Shake Flask Cultiv...mentioning

confidence: 99%

Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems

Schütte,

Hanisch,

Scheler

et al. 2024

Appl Microbiol Biotechnol

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The triterpene squalene is widely used in the food, cosmetics and pharmaceutical industries due to its antioxidant, antistatic and anti-carcinogenic properties. It is usually obtained from the liver of deep sea sharks, which are facing extinction. Alternative production organisms are marine protists from the family Thraustochytriaceae, which produce and store large quantities of various lipids. Squalene accumulation in thraustochytrids is complex, as it is an intermediate in sterol biosynthesis. Its conversion to squalene 2,3-epoxide is the first step in sterol synthesis and is heavily oxygen dependent. Hence, the oxygen supply during cultivation was investigated in our study. In shake flask cultivations, a reduced oxygen supply led to increased squalene and decreased sterol contents and yields. Oxygen-limited conditions were applied to bioreactor scale, where squalene accumulation and growth of Schizochytrium sp. S31 was determined in batch, fed-batch and continuous cultivation. The highest dry matter (32.03 g/L) was obtained during fed-batch cultivation, whereas batch cultivation yielded the highest biomass productivity (0.2 g/L*h−1). Squalene accumulation benefited from keeping the microorganisms in the growth phase. Therefore, the highest squalene content of 39.67 ± 1.34 mg/g was achieved by continuous cultivation (D = 0.025 h−1) and the highest squalene yield of 1131 mg/L during fed-batch cultivation. Volumetric and specific squalene productivity both reached maxima in the continuous cultivation at D = 0.025 h−1 (6.94 ± 0.27 mg/L*h−1 and 1.00 ± 0.03 mg/g*h−1, respectively). Thus, the choice of a suitable cultivation method under oxygen-limiting conditions depends heavily on the process requirements. Key points • Measurements of respiratory activity and backscatter light of thraustochytrids • Oxygen limitation increased squalene accumulation in Schizochytrium sp. S31 • Comparison of different cultivation methods under oxygen-limiting conditions

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Section: Growth Monitoring By Backscatter Light In Shake Flask Cultiv...mentioning

confidence: 99%

Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems

Schütte,

Hanisch,

Scheler

et al. 2024

Appl Microbiol Biotechnol

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show abstract

Dual stress factors adaptive evolution for high EPA production in Schizochytrium sp. and metabolomics mechanism analysis

Ou,

Qin,

Feng

et al. 2024

Bioprocess Biosyst Eng

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Application and research progress of ARTP mutagenesis in actinomycetes breeding

Zhu,

Ding,

Rang

et al. 2024

Gene

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Transcriptome Analysis Reveals an Eicosapentaenoic Acid Accumulation Mechanism in a Schizochytrium sp. Mutant

Abstract: Schizochytrium , a marine eukaryotic microorganism, has emerged as a candidate for the commercial production of PUFAs. EPA is an omega-3 PUFA with preventive and therapeutic effects against cardiovascular diseases, schizophrenia, and other disorders.

Cited by 4 publications

References 53 publications

Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems

Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems

Dual stress factors adaptive evolution for high EPA production in Schizochytrium sp. and metabolomics mechanism analysis

Application and research progress of ARTP mutagenesis in actinomycetes breeding

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