Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry

Shiva, Sunitha; Vu, Hieu; Roth, Mary R.; Zhou, Zhenguo; Marepally, Shantan R.; Nune, Daya Sagar; Lushington, Gerald H.; Visvanathan, Mahesh; Welti, Ruth

doi:10.1007/978-1-62703-401-2_9

Cited by 38 publications

(31 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification and quantification of individual lipid species in biological samples has been greatly facilitated by recent advances in LC-MS and GC-MS [29]. These techniques have been successfully employed for the analysis of plant lipids [30], [31], including under normal versus physiologically-stressed conditions [32], and enabled the characterization of MGDG and DGDG in photosynthetic marine protists [33], [34], and SQDG [35], betaine lipids [16] and TAGs [14], [36] in various species of algae.…”

Section: Introductionmentioning

confidence: 99%

Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

et al. 2014

View full text Add to dashboard Cite

Many species of microalgae produce greatly enhanced amounts of triacylglycerides (TAGs), the key product for biodiesel production, in response to specific environmental stresses. Improvement of TAG production by microalgae through optimization of growth regimes is of great interest. This relies on understanding microalgal lipid metabolism in relation to stress response in particular the deprivation of nutrients that can induce enhanced TAG synthesis. In this study, a detailed investigation of changes in lipid composition in Chlorella sp. and Nannochloropsis sp. in response to nitrogen deprivation (N-deprivation) was performed to provide novel mechanistic insights into the lipidome during stress. As expected, an increase in TAGs and an overall decrease in polar lipids were observed. However, while most membrane lipid classes (phosphoglycerolipids and glycolipids) were found to decrease, the non-nitrogen containing phosphatidylglycerol levels increased considerably in both algae from initially low levels. Of particular significance, it was observed that the acyl composition of TAGs in Nannochloropsis sp. remain relatively constant, whereas Chlorella sp. showed greater variability following N-deprivation. In both algae the overall fatty acid profiles of the polar lipid classes were largely unaffected by N-deprivation, suggesting a specific FA profile for each compartment is maintained to enable continued function despite considerable reductions in the amount of these lipids. The changes observed in the overall fatty acid profile were due primarily to the decrease in proportion of polar lipids to TAGs. This study provides the most detailed lipidomic information on two different microalgae with utility in biodiesel production and nutraceutical industries and proposes the mechanisms for this rearrangement. This research also highlights the usefulness of the latest MS-based approaches for microalgae lipid research.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Since the tandem MS-based shotgun lipidomics is straightforward and have many advantages, this methodology has become very important to analyze many specific lipid classes. 111-113 …”

Section: Esi-ms Of Lipid Analysismentioning

confidence: 99%

Applications of mass spectrometry for cellular lipid analysis

Wang

Han

2015

Mol. BioSyst.

View full text Add to dashboard Cite

Mass spectrometric analysis of cellular lipids is an enabling technology for lipidomics, which is a rapidly-developing research field. In this review, we briefly discuss the principles, advantages, and possible limitations of electrospray ionization (ESI) and matrix assisted laser desorption/ionization (MALDI) mass spectrometry-based methodologies for the analysis of lipid species. The applications of these methodologies to lipidomic research are also summarized.

show abstract

“…Additional recommendations and options for extraction have been provided previously in another article in this series (14).…”

Section: Sampling Lipid Extraction and Dry Mass Measurementmentioning

confidence: 99%

“…Lipid extracts from cold-treated plants may be introduced to a mass spectrometer by direct infusion or by liquid chromatography, and both methods have been utilized in Arabidopsis cold stress studies (3,12,13). Here we describe a direct infusion approach similar to that used to analyze lipids during cold acclimation and freezing stress in recent work by Vu et al (13) and extend our previously described analytical approach (3,14). The procedure takes advantage of the automated capability for processing of triple quadrupole mass spectral data at LipidomeDB Data Calculation Environment (15).…”

Section: Introductionmentioning

confidence: 96%

A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Shiva

Hall

et al. 2014

Methods in Molecular Biology

Self Cite

View full text Add to dashboard Cite

Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry

Cited by 38 publications

References 9 publications

Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

Applications of mass spectrometry for cellular lipid analysis

A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Contact Info

Product

Resources

About