A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Vu, Hieu; Shiva, Sunitha; Hall, Aaron Smalter; Welti, Ruth

doi:10.1007/978-1-4939-0844-8_15

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…The gradient method was modified from Ref. 53. The sample was infused into the source using a constant flow of 100% solvent A (methanol) from 0 to 10 min.…”

Section: Quantification Of Lipids Using Msmentioning

confidence: 99%

“…Re-equilibration was achieved with 100% solvent A from 12.1 to 15 min. Samples were dissolved in 100 l of isopropyl alcohol, chloroform, methanol, 300 mM ammonium acetate in water, 25:30:41.5:3.5, v/v/v/v) (53). Sphingolipids were detected in the positive ion mode with the following instrument settings: curtain gas, 20 p.s.i.…”

Section: Quantification Of Lipids Using Msmentioning

confidence: 99%

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Species-specific differences in nonlysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations

Woeste

Stern

Raju

et al. 2019

Journal of Biological Chemistry

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The nonlysosomal glucosylceramidase ␤2 (GBA2) catalyzes the hydrolysis of glucosylceramide to glucose and ceramide. Mutations in the human GBA2 gene have been associated with hereditary spastic paraplegia (HSP), autosomal-recessive cerebellar ataxia (ARCA), and the Marinesco-Sjögren-like syndrome. However, the underlying molecular mechanisms are ill-defined. Here, using biochemistry, immunohistochemistry, structural modeling, and mouse genetics, we demonstrate that all but one of the spastic gait locus #46 (SPG46)-connected mutations cause a loss of GBA2 activity. We demonstrate that GBA2 proteins form oligomeric complexes and that proteinprotein interactions are perturbed by some of these mutations. To study the pathogenesis of GBA2-related HSP and ARCA in vivo, we investigated GBA2-KO mice as a mammalian model system. However, these mice exhibited a high phenotypic variance and did not fully resemble the human phenotype, suggesting that mouse and human GBA2 differ in function. Whereas some GBA2-KO mice displayed a strong locomotor defect, others displayed only mild alterations of the gait pattern and no signs of cerebellar defects. On a cellular level, inhibition of GBA2 activity in isolated cerebellar neurons dramatically affected F-actin dynamics and reduced neurite outgrowth, which has been associated with the development of neurological disorders. Our results shed light on the molecular mechanism underlying the pathogenesis of GBA2-related HSP and ARCA and reveal species-specific differences in GBA2 function in vivo.

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“…The gradient method was modified from Ref. 53. The sample was infused into the source using a constant flow of 100% solvent A (methanol) from 0 to 10 min.…”

Section: Quantification Of Lipids Using Msmentioning

confidence: 99%

Section: Quantification Of Lipids Using Msmentioning

confidence: 99%

Species-specific differences in nonlysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations

Woeste

Stern

Raju

et al. 2019

Journal of Biological Chemistry

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“…Our main goal was to elucidate in what way the plant lipidome responds to FR light, which eventually leads to a significant improvement in the freezing tolerance of plants. Applying the most up-to-date lipidomic analysis technique (electrospray ionization triple quadrupole mass spectrometry [ 78 ]), we could determine how the membrane lipid species are quantitatively and qualitatively altered under different temperature and light conditions. This study demonstrates that WL+FR can substitute cold treatment at higher temperature (15 °C), and mostly acts in a synergistic manner at cold (5 °C) nonfreezing temperature.…”

Section: Introductionmentioning

confidence: 99%

Decreased R:FR Ratio in Incident White Light Affects the Composition of Barley Leaf Lipidome and Freezing Tolerance in a Temperature-Dependent Manner

Kovács

Ahres

Pálmai

et al. 2020

IJMS

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In cereals, C-repeat binding factor genes have been defined as key components of the light quality-dependent regulation of frost tolerance by integrating phytochrome-mediated light and temperature signals. This study elucidates the differences in the lipid composition of barley leaves illuminated with white light or white light supplemented with far-red light at 5 or 15 °C. According to LC-MS analysis, far-red light supplementation increased the amount of monogalactosyldiacylglycerol species 36:6, 36:5, and 36:4 after 1 day at 5 °C, and 10 days at 15 °C resulted in a perturbed content of 38:6 species. Changes were observed in the levels of phosphatidylethanolamine, and phosphatidylserine under white light supplemented with far-red light illumination at 15 °C, whereas robust changes were observed in the amount of several phosphatidylserine species at 5 °C. At 15 °C, the amount of some phosphatidylglycerol species increased as a result of white light supplemented with far-red light illumination after 1 day. The ceramide (42:2)-3 content increased regardless of the temperature. The double-bond index of phosphatidylglycerol, phosphatidylserine, phosphatidylcholine ceramide together with total double-bond index changed when the plant was grown at 15 °C as a function of white light supplemented with far-red light. white light supplemented with far-red light increased the monogalactosyldiacylglycerol/diacylglycerol ratio as well. The gene expression changes are well correlated with the alterations in the lipidome.

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“…Metabolomics has been broadly applied to a myriad of studies pertaining to the metabolic responses of Arabidopsis to various abiotic or biotic stresses, including heat, freezing, drought, and salinity [12,13]. Lipidomics, as a comparably young branch in the realm of metabolomics area, has emerged as a burgeoning arena within the last few years [14]; and has been applied to various analyses to elucidate the physiological mechanisms of Arabidopsis [15,16,17,18,19,20]. …”

Section: Introductionmentioning

confidence: 99%

Metabolomics, a Powerful Tool for Agricultural Research

Tian

Lam

Shui

2016

IJMS

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Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in biological samples. As complements to transcriptomics and proteomics, it has played pivotal roles in agricultural and food science research. In this review, we discuss the capacities of NMR, GC/LC-MS in the acquisition of plant metabolome, and address the potential promise and diverse applications of metabolomics, particularly lipidomics, to investigate the responses of Arabidopsis thaliana, a primary plant model for agricultural research, to environmental stressors including heat, freezing, drought, and salinity.

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A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Cited by 9 publications

References 17 publications

Species-specific differences in nonlysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations

Species-specific differences in nonlysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations

Decreased R:FR Ratio in Incident White Light Affects the Composition of Barley Leaf Lipidome and Freezing Tolerance in a Temperature-Dependent Manner

Metabolomics, a Powerful Tool for Agricultural Research

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