In this study, the dyeing procedure of wool fibers withPrangos ferulaceawas evaluated and optimized by response surface methodology (RSM). Using this method, the quantitive relationship between dye concentration ofPrangos ferulacea, mordant concentration, dyeing temperature, and dyeing time on the dyeing procedure was investigated. The effect of these variables as well as plasma pretreatment was examined on the color strength of dyed samples. Finally, the fastness characteristic of dye sampled at proposed optimized condition was reported. The obtained results indicate that the presence of mordant improved the fastness properties and dyes uptake of wool fibers.