Lipid profiling of follicular fluid from women undergoing IVF: Young poor ovarian responders versus normal responders

Cataldi, Thaís Regiani; Cordeiro, Fernanda Bertuccez; Costa, Lívia do Vale Teixeira da; Pilau, Eduardo Jorge; Ferreira, Christina R.; Gozzo, Fábio C.; Eberlin, Marcos N.; Bertolla, Ricardo Pimenta; Cedenho, A.P.; Turco, E.G. Lo

doi:10.3109/14647273.2013.852255

Cited by 31 publications

(27 citation statements)

References 31 publications

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“…Thus, the overrepresentation of PG may be indirectly related to apoptosis. In another study from our group, it was possible to infer that PG may be related to the balance in the follicular microenvironment, protecting oocytes against damaging molecular processes and playing a role in ovarian functioning [28].…”

Section: Discussionmentioning

confidence: 98%

Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Cordeiro

Cataldi

Costa

et al. 2014

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 98%

Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Cordeiro

Cataldi

Costa

et al. 2014

J Assist Reprod Genet

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“…Scarce information is available on the PL content of FF and its relation to embryo development. Several phosphocholines were found to show lower accumulation in poor ovarian responder patients (18). Choline and phosphocholine, precursors of phosphatidylcholines, also showed a differential accumulation in the FF of oocytes that developed into early cleavage-stage embryos (4).…”

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confidence: 95%

A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

et al. 2019

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Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive‐outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive‐outcome patients, whereas derivatives of vitamin D were highly accumulated in positive‐outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive‐outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck‐Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben‐Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). http://www.fasebj.org

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“…Lipids were extracted using the method reported by Bligh and Dyer () with modifications, as reported elsewhere (Cataldi et al, ). Briefly, the catheter tip was placed in a microtube, into which 150 ml of ultrapure water was added, followed by the addition of 190 μl of chloroform and 375 μl of methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Lipids were extracted using the method reported by Bligh and Dyer (1959) with modifications, as reported elsewhere (Cataldi et al, 2013).…”

Section: Sample Preparation Lipid Extraction and Mass Spectrometrymentioning

confidence: 99%

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

Braga

Borges

Godoy

et al. 2018

Molecular Reproduction Devel

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For the present study we asked whether the endometrial fluid lipidomic may be a useful approach to predict endometrial receptivity in freeze‐all cycles. For this case‐control study, endometrial fluid samples were collected from 41 patients undergoing freeze‐all cycles. Samples were split depending on the pregnancy outcome: positive group (n = 24) and negative group (n = 17). Data were acquired by the matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS‐DA) were applied. A list of potential biomarker ion ratios was obtained and the values were used to build a receiver operating characteristic (ROC) curve to predict pregnancy success. The lipid categories were attributed by LIPID MAPS database. Ion ratios were established according to their correlations and used for the analysis. The PCA showed a tendency of separation between the studied groups, whereas the PLS‐DA was able to clearly distinguish them. Fifteen ratios (13 hyper‐represented in the negative and two hyper‐represented in the positive group) were selected according to their importance for model prediction. These ratios were used to build the ROC curve, which presented an area under curve of 84.0% (95%CI: 69.2–97.4%; p = 0.009). These findings suggest that lipidomic profiling of endometrial fluid may be a valuable tool for identifying the time interval comprising the window of implantation.

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Lipid profiling of follicular fluid from women undergoing IVF: Young poor ovarian responders versus normal responders

Cited by 31 publications

References 31 publications

Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

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