Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Cordeiro, Fernanda Bertuccez; Cataldi, Thaís Regiani; Costa, Lívia do Vale Teixeira da; Lima, Camila Bruna de; Stevanato, J.; Zylbersztejn, Daniel Suslik; Ferreira, Christina R.; Eberlin, Marcos N.; Cedenho, A.P.; Turco, E.G. Lo

doi:10.1007/s10815-014-0375-0

Cited by 21 publications

(19 citation statements)

References 43 publications

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“…Although modulation of different PLs was observed, the most important and statistically validated finding in both superstimulatory treatments was the higher relative abundance of the PC (34:2) in the follicular fluid from superstimulated cows. Interestingly, the overrepresentation by the PC (34:2) was also observed by electrospray ionization mass spectrometry (ESI-MS) in the follicular fluid of women with polycystic ovary syndrome (PCOS) and a hyper-response to gonadotropin [37].…”

Section: Discussionmentioning

confidence: 95%

Lipid profiles of follicular fluid from cows submitted to ovarian superstimulation

et al. 2017

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Ovarian superstimulation with exogenous gonadotropins has been extensively used to produce in vivo-derived embryos for embryo transfer in cattle. This process modifies the antral follicle microenvironment and affects oocyte and embryo quality as well the differentiation of granulosa cells. Lipids play significant roles in the cell, such as energy storage, cell structure, and fine-tuning of the physical properties and functions of biological membranes. The phospholipid (PL) contents as well as the effects of superstimulatory treatments on the PL profile of follicular fluid from cows, however, remain unknown. Therefore, to gain insight into the effects of superstimulation with follicle-stimulating hormone (FSH; P-36 protocol) or FSH combined with equine chorionic gonadotropin (eCG; P-36/eCG protocol) on the profile and abundance of PL from cows submitted or not submitted to superstimulatory protocols, were treated with these two superstimulatory protocols. As a control, non-superstimulated cows were only submitted to estrous synchronization. The follicular fluid was aspirated, the remaining cells removed and the follicular fluid stored at -80 °C until extraction. The lipid screening was performed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and this technique allowed the identification of sphingomyelins (SM) and phosphatidylcholines (PC) and phosphoethanolamines (PE). The relative abundance of the ions observed in the three experimental groups was analyzed by multivariate and univariate statistical models. The phospholipid SM (16:0) and PC (36:4) and/or PC (34:1) were less (P < 0.05) abundant in the P-36 group compared to the control or P-36/eCG groups. However, the PC (34:2) was more (P < 0.05) abundant in both group of superstimulated cows compared to the control. In summary, ovarian superstimulation seems to modulate the PL content of bovine follicular fluid with a significant increase in PC (34:2), which jointly with others PC and SM, seems to offer a suitable biomarker involved with reproductive processes successful as ovary superstimulation response and embryo development.

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Section: Discussionmentioning

confidence: 95%

Lipid profiles of follicular fluid from cows submitted to ovarian superstimulation

et al. 2017

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“…The discriminant transitions were tentatively attributed as glycerophospholipids, which should have an important role in keeping a balanced follicular environment as indicated by higher abundances of most lipids in the control group. Moreover, these lipid classes were previously detected by ESI‐MS in FF of controls and related to optimal FF environment found based on the exploration of only positive mode ionization. The present study included experiments in both ion modes using Prec and NL scans and the most discriminant molecular features were found in negative ion mode (Methods I and II).…”

Section: Resultsmentioning

confidence: 97%

“…Small molecules have been reported as accurate biomarkers for diseases onset and progression and mass spectrometry technologies are rapidly evolving to the analysis of small size and salty samples in complex systems . The search for biomarkers in the FF of PCOS patients undergoing IVF is a cornerstone for understanding how this syndrome affects the ovaries and how it compromises fertility potential to the extent that these patients need ART treatments.…”

Section: Resultsmentioning

confidence: 99%

“…It has been previously reported that ovarian superovulation alters the FF lipid composition in cows . In addition, although lipids have been suggested to be indicative of PCOS diagnosis, molecular diagnosis of this condition remains a challenge as it is made in the absence of specific symptoms and without validated molecular markers …”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, this type of sample may contain potential biomarkers . Several biomarkers in FF have recently been proposed for PCOS diagnosis using mass spectrometry (MS) approaches, such as gas chromatography coupled to MS (GC/MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS), and ESI‐MS with flow injection . The present study uses MRM‐profiling to interrogate the FF of patients diagnosed with PCOS in comparison with controls.…”

Section: Introductionmentioning

confidence: 99%

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Multiple reaction monitoring (MRM)‐profiling for biomarker discovery applied to human polycystic ovarian syndrome

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Nontargeted metabolomics analysis of follicular fluid in patients with endometriosis provides a new direction for the study of oocyte quality

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et al. 2023

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Endometriosis is a common, estrogen‐dependent chronic gynecological disease that endangers the reproductive system and systemic metabolism of patients. We aimed to investigate the differences in metabolic profiles in the follicular fluid between infertile patients with endometriosis and controls. A total of 25 infertile patients with endometriosis and 25 infertile controls who were similar in age, BMI, fertilization method and ovulation induction treatment were recruited in this study. Metabolomics analysis of follicular fluid was performed by two methods of high‐performance liquid chromatography tandem mass spectrometry. There were 36 upregulated and 17 downregulated metabolites in the follicular fluid of patients in the endometriosis group. KEGG pathway analysis revealed that these metabolites were enriched in phenylalanine, tyrosine and tryptophan biosynthesis, aminoacyl‐tRNA biosynthesis, phenylalanine metabolism and pyrimidine metabolism pathways. A biomarker panel consisting of 20 metabolites was constructed by random forest, with an accuracy of 0.946 and an AUC of 0.988. This study characterizes differences in follicular fluid metabolites and associated pathway profiles in infertile patients with endometriosis. These findings can provide a better comprehensive understanding of the disease and a new direction for the study of oocyte quality, as well as potential metabolic markers for the prognosis of endometriosis.

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Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Cited by 21 publications

References 43 publications

Lipid profiles of follicular fluid from cows submitted to ovarian superstimulation

Lipid profiles of follicular fluid from cows submitted to ovarian superstimulation

Multiple reaction monitoring (MRM)‐profiling for biomarker discovery applied to human polycystic ovarian syndrome

Nontargeted metabolomics analysis of follicular fluid in patients with endometriosis provides a new direction for the study of oocyte quality

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