Lipid profiles of acid-tolerant mutants of the green microalga Chlorella saccharophila reveal hydrocarbons and high-value lipids with potential industrial applications

Teco-Bravo, Jalsen Iván; Barahona-Pérez, Luis Felipe; Peraza-Echeverría, Santy; Baas-Espinola, Fray Martin; Reyes-Sosa, Carlos Francisco; Herrera-Valencia, Virginia Aurora

doi:10.1016/j.biteb.2021.100636

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Moreover, it has the potential of producing high-value compounds, such as lipid, pigments and proteins, which makes them a promising platform as expression system of heterologous proteins. [6][7] Genetically tractable unicellular green algae Chlamydomonas reinhardtii was the first to apply for delivery of foreign DNA into its chloroplast via particle bombardment method, [8] and C. reinhardtii was regarded as the model organism in the beginning. [9] Microalgae Chlorella has competitive advantages such as easy to culture and produce bio-compound in large scale made them the popular chassis as well.…”

Section: Introductionmentioning

confidence: 99%

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CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production

Lin

2021

Biotechnology Journal

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Chlorella species are indispensable microalgae for biorefinery but are hardly in DNA manipulation due to the high guanine‐cytosine (GC) contents of DNA. In this study, we established a new approach via 20 guanines for sgRNA design, which is annotated as “Adaptive Single Guide Assisted Regulation DNA (ASGARD)” and coupling with CRISPR interference associated dCas9 system to overcome the difficulties. At first, C. sorokiniana was predominate as its faster growth rate when compared to C. vulgaris and C. variabilis in the culture using Tris‐acetate‐phosphate (TAP) medium. Among all the genetic transformants, gene regulation via CRISPRa‐VP64 (CRISPRa) enhanced the protein contents up to 60% (w/w) of dry cell weight, where the highest concentration was 570 mg L‐1. Meanwhile, CRISPRi‐KRAB (CRISPRi) with ASGARD increased protein content to 65% and lipid formed in the range of 150–250 mg L‐1. From the transcriptome analysis, we deciphered 468 genes down‐regulated and 313 genes up‐regulated via CRISPRi, while less difference existed in CRISPRa. This novel design and technology reveal a high potential of gene‐regulating approach to other species for the biorefinery and bio‐industry.

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Section: Introductionmentioning

confidence: 99%

“…Moreover, it has the potential of producing high‐value compounds, such as lipid, pigments and proteins, which makes them a promising platform as expression system of heterologous proteins. [ 6–7 ]…”

Section: Introductionmentioning

confidence: 99%

CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production

Lin

2021

Biotechnology Journal

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Microalgae and Cyanobacteria: A Potential Source for Drug Discovery Using Genome Mining Approach

Lamare¹,

Chaurasia²

2022

Clean Energy Production Technologies

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The Recent Advanced in Microalgal Phytosterols: Bioactive Ingredients Along With Human-Health Driven Potential Applications

Sañé

Mondo

Ambrosino

et al. 2021

Food Reviews International

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Lipid profiles of acid-tolerant mutants of the green microalga Chlorella saccharophila reveal hydrocarbons and high-value lipids with potential industrial applications

Cited by 3 publications

References 16 publications

CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production

CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production

Microalgae and Cyanobacteria: A Potential Source for Drug Discovery Using Genome Mining Approach

The Recent Advanced in Microalgal Phytosterols: Bioactive Ingredients Along With Human-Health Driven Potential Applications

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