2021
DOI: 10.1038/s41598-021-90870-8
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Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Abstract: Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the sam… Show more

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Cited by 24 publications
(17 citation statements)
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References 66 publications
(73 reference statements)
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“…Experimental heifers were subjected to estrus synchronization, as described by Janati Idrissi et al. [ 3 ]. Briefly, synchronization treatments were performed by insertion of an intravaginal progesterone releasing device (Prid ® Delta, 1.55 g, Ceva, Libourne, France) and this was followed, 6 days later, by 2 mL intramuscular injection of a prostaglandin F2 alpha analog (Estrumate ® , MSD Santé Animale — Intervet, Beaucouzé, France; equivalent to 0.5 mg cloprostenol).…”
Section: Methodsmentioning
confidence: 99%
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“…Experimental heifers were subjected to estrus synchronization, as described by Janati Idrissi et al. [ 3 ]. Briefly, synchronization treatments were performed by insertion of an intravaginal progesterone releasing device (Prid ® Delta, 1.55 g, Ceva, Libourne, France) and this was followed, 6 days later, by 2 mL intramuscular injection of a prostaglandin F2 alpha analog (Estrumate ® , MSD Santé Animale — Intervet, Beaucouzé, France; equivalent to 0.5 mg cloprostenol).…”
Section: Methodsmentioning
confidence: 99%
“…For Holstein heifers, ovarian stimulation was performed as previously described [ 3 ] 36 h after the removal of the dominant follicles. Briefly, a new intravaginal progesterone device (Prid ® Delta, 1.55 g, Ceva, Libourne, France) was inserted, and the ovarian stimulation was performed by five intramuscular injections of decreasing porcine follicle stimulating hormone/porcine luteinizing hormone (pFSH/pLH) doses (Stimulfol ® , Reprobiol, Ouffet, Belgium), every 12 h, over 2.5 days.…”
Section: Methodsmentioning
confidence: 99%
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