Basic and applied features in the cryopreservation progress of bovine embryos

Valente, Roniele Santana; Marsico, Thamiris Vieira; Sudano, Mateus José

doi:10.1016/j.anireprosci.2022.106970

Cited by 16 publications

(7 citation statements)

References 56 publications

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“…These include oscillating temperature, DNA damage or DNA damaging agents, osmotic stress, and the availability of organic osmolytes, oxygen and oxidative stress, hyperglycemia and carbon substrate availability, hyperlipidemia and oxidized lipids, calcium ionophores, cytokines, amino acid deprivation, insulin signaling, and serum components [ 15 , 30 , 31 , 32 ]. Cold stress associated with cryopreservation affects embryo development and gene regulation [ 27 , 29 , 33 ]. These adverse factors negatively impact ER functions and protein synthesis and folding, resulting in the activation of ER stress and the UPR signaling pathways in in vitro-produced embryos [ 14 , 15 , 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been studied that the in vitro-produced embryo (IVPE) has distinct characteristics when compared with its counterpart produced in vivo. These differences between IVPE and in vivo derived from embryos involve morphological [ 27 ] and molecular aspects that affect the embryo quality and development [ 28 ], decreasing cryo-tolerance and pregnancy rates [ 29 ]. Therefore, maintaining cell viability after warming is a prerequisite to achieving high outcomes within cryopreservation protocols.…”

Section: Introductionmentioning

confidence: 99%

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Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance

Pioltine,

Costa,

Franchi

et al. 2023

IJMS

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During embryo development, the endoplasmic reticulum (ER) acts as an important site for protein biosynthesis; however, in vitro culture (IVC) can negatively affect ER homeostasis. Therefore, the aim of our study was to evaluate the effects of the supplementation of tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor, in the IVC of bovine embryos. Two experiments were carried out: Exp. 1: an evaluation of blastocyst rate, hatching kinetics, and gene expression of hatched embryos after being treated with different concentrations of TUDCA (50, 200, or 1000 μM) in the IVC; Exp. 2: an evaluation of the re-expansion, hatching, and gene expression of hatched embryos previously treated with 200 µM of TUDCA at IVC and submitted to vitrification. There was no increase in the blastocyst and hatched blastocyst rates treated with TUDCA in the IVC. However, embryos submitted to vitrification after treatment with 200 µM of TUDCA underwent an increased hatching rate post-warming together with a down-regulation in the expression of ER stress-related genes and the accumulation of lipids. In conclusion, this work showed that the addition of TUDCA during in vitro culture can improve the cryotolerance of the bovine blastocyst through the putative modulation of ER and oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance

Pioltine,

Costa,

Franchi

et al. 2023

IJMS

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“…More than one and a half million bovine embryos were produced in vitro worldwide in 2021 2 , but 60% had to be transferred fresh due to the lack of a reliable cryopreservation method that results in favorable pregnancy rates 2,7,8 . Consequently, a large number of embryo recipient animals must be available, resulting in increased costs 9,10 .…”

Section: Embryo Morphokinetics Derived From Fresh and Vitrified Bovin...mentioning

confidence: 99%

Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2023

Sci Rep

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Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially the timing of embryonic divisions and aberrant zygotic cleavage patterns. Bovine embryo development is impaired after oocyte vitrification, but little is known about the underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) and vitrified oocytes (n = 182) were monitored by time-lapse imaging and the timing and nature of early blastomere divisions were modeled to find associations with blastocyst development at day 8. The predictive potential of morphokinetic parameters was analyzed by logistic regression and receiver operating characteristic curve analysis to determine optimal cut-off values. Lag-phase was highly correlated with embryo development. Remarkably, 100% of zygotes that reached the blastocyst stage showed a lag-phase. Fast first cleavage increased the chance of blastocyst development to 30% with a cut-off of 32 h and 22 min. Aberrant zygotic cleavage events, including multipolar division, unequal blastomere sizes, and membrane ruffling resulted in decreased blastocyst development. Multipolar division leads to uneven blastomeres, which was associated with anuclear and multinuclear blastomeres, indicating genome segregation errors. Moreover, we described for the first time morphokinetics of embryos derived from vitrified bovine oocytes. Vitrification severely affected blastocyst development, although lower cryoprotectant concentration in equilibration solutions seems to be less detrimental for embryo yield. Impaired development was linked to slow cleavages, lower lag-phase incidence, and increased early embryonic arrest. Typically, less than 15% of the embryos produced from vitrified oocytes reached more than eight cells. Interestingly, the rate of abnormal first cleavage events was not affected by oocyte vitrification. In conclusion, time to first cleavage, the presence of a lag-phase, and the absence of aberrant zygotic cleavage were the best predictors of bovine blastocyst development for both fresh and vitrified oocytes.

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“…Space limitations mean that some issues are not included or are discussed in a limited fashion. The reader is directed elsewhere ( Sirard, 2016 ; Ealy et al, 2019 ; Hansen, 2020a and 2020b; Baruselli et al, 2021 ; Valente et al, 2022 ) for treatment of some relevant topics not discussed here, such as recipient management, cryopreservation, and the impact of sire and genetics, as well as for a more detailed analysis of specific topics discussed here.…”

Section: Toward the Goal Of An In Vitro -Produced ...mentioning

confidence: 99%

Review: Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production

Hansen

2023

animal

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Basic and applied features in the cryopreservation progress of bovine embryos

Cited by 16 publications

References 56 publications

Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance

Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance

Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Review: Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production

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