Lipid peroxidation in human spermatozoa as relatd to midpiece abnormalities and motility

Rao, BS; Soufir, J. C.; Martı́n, M. Elena; David, G

doi:10.1002/mrd.1120240202

Cited by 291 publications

(153 citation statements)

References 16 publications

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“…Similar observations have been made in humans (Rao et al, 1989;Verma and Kanwar, 1999), buffalo (Singh et al, 1989) and mice (Kodama et al, 1996). It is known that ferrous ions act as promoters to initiate the chain of LPO, thereby inducing the oxidative stress in sperm membranes (Sharma and Agarwal, 1996).…”

Section: Discussionsupporting

confidence: 76%

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Bansal

Bilaspuri

2008

Animal

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Numerous factors influence male fertility, one of these being the oxidative stress, which has elicited enormous interest recently. In sperm, induction of oxidation decreases motility and viability but increases lipid peroxidation (LPO). The optimum dose of ferrous ascorbate (FeAA: FeSO 4 1 ascorbic acid) for inducing oxidative stress by affecting motility, viability and LPO has been ascertained in local crossbred cattle bull spermatozoa. The fractions of spermatozoa suspended in 2.9% sodium citrate were subjected to three doses of FeAA (100 : 500, 150 : 750, 200 : 1000; mmol/l FeSO 4 : mmol/l ascorbic acid). These fractions were assessed for various parameters. Increase in the incubation period and promoter concentration induced a decrease in motility and viability, but an increase in LPO. Among three doses of FeAA, 150 : 750 mmol/l ascorbic acid is suggested to be the optimum/best dose as it induces the oxidative stress/LPO to a significant extent and also maintains better motility and viability as compared with the other two doses, and such conditions may enhance the fertilising potential of bull spermatozoa.

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Section: Discussionsupporting

confidence: 76%

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Bansal

Bilaspuri

2008

Animal

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“…Spermatozoa are particularly sensitive to ROS assault because of the existence of high quantities of polyunsatured fatty acids in their own membranes and the lack of capacity for DNA repair (Aitken & Clarkson, 1987;Álvarez & Storey, 1995;Kim & Parthasanthy, 1998;Donnelly et al, 2000). Increased ROS levels have been correlated with decreased sperm motility (Álvarez & Story, 1982;Aitken et al, 1989;Iwasaki & Gagnon, 1992; Jedlinska-Krakowska et al, 2006), retention of excess residual cytoplasm (Baker & Aitken, 2005), midpiece abnormalities (Rao et al, 1989), abnormal morphology (Aitken et al, 1994), DNA damage in the male germ line cells that is associated with mutation, poor fertilization rates following in vitro fertilization, defective pre-implantation embryonic development and high rates of miscarriage in the offspring, and fi nally, increased incidence of birth defects and genetic diseases (Álvarez & Storey, 1982;Fraga et al, 1991;Aitken et al, 1998;Lim et al, 1998;Agarwal et al, 2008). These problems, associated with sperm DNA damage, result in defective spermatogenesis (Agarwal et al, 2008).…”

Section: Infertility Oxidative Stress and Vitamin Cmentioning

confidence: 99%

Vitamin C and oxidative stress in the seminiferous epithelium

et al. 2011

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In this article, we focus on the fundamental role of vitamin C transporters for the normal delivery of vitamin C to germ cells in the adluminal compartment of seminiferous tubules. We argue that the redox status within spermatozoa or in semen is partly responsible for the etiology of infertility. In this context, antioxidant defence plays a critical role in male fertility. Vitamin C, a micronutrient required for a wide variety of metabolic functions, has long been associated with male reproduction. Two systems for vitamin C transport have been described in mammals. Facilitative hexose transporters (GLUTs), with 14 known isoforms to date, GLUT1-GLUT14, transport the oxidized form of vitamin C (dehydroascorbic acid) into the cells. Sodium ascorbic acid co-transporters (SVCTs), SVCT1 and SVCT2 transport the reduced form of vitamin C (ascorbic acid). Sertoli cells control germ cell proliferation and differentiation through cell-cell communication and form the blood-testis barrier. Because the blood-testis barrier limits direct access of molecules from the plasma into the adluminal compartment of the seminiferous tubule, one important question is the method by which germ cells obtain vitamin C. Some interesting results have thrown light on this matter. Expression of SVCT2 and some isoforms of GLUT transporters in the testis have previously been described. Our group has demonstrated that Sertoli cells express functionally active vitamin C transporters. Kinetic characteristics were described for both transport systems (SVCT and GLUT systems). Sertoli cells are able to transport both forms of vitamin C. These fi ndings are extremely relevant, because Sertoli cells may control the amount of vitamin C in the adluminal compartment, as well as regulating the availability of this metabolite throughout spermatogenesis.

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“…Lack of peroxidase activity in the sperm may result in the accumulation of reactive oxygen species and in oxidative damage to the mitochondria (26,(37)(38)(39). Alternatively, absence of the protein may affect formation of the sperm mitochondrial capsule that is essential for forming the mitochondrial helix in the sperm mid-piece.…”

Section: Apoer2 In Sperm Developmentmentioning

confidence: 99%

Essential Role of the Apolipoprotein E Receptor-2 in Sperm Development

Andersen

Yeung²,

Vorum

et al. 2003

Journal of Biological Chemistry

103

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The apolipoprotein (apo) E receptor-2 (apoER2) is a member of the low density lipoprotein receptor gene family and an important regulator of neuronal migration. It acts as a receptor for the signaling factor Reelin and provides positional cues to neurons that migrate to their proper position in the developing brain. Besides brain formation defects, apoER2-deficient mice also exhibit male infertility. The role of the receptor in male reproduction, however, remained unclear. Here we demonstrate that apoER2 is highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. Reduced PHGPx expression in apoER2 knockout mice results in the inability of the sperm to regulate the cell volume and in abnormal sperm morphology and immotility. Because insufficient expression of PHGPx is a major cause of infertility in men, these findings not only highlight an important new function for apoER2 that is unrelated to neuronal migration, but they also suggest a possible role for apoER2 in human infertility.Apolipoprotein (apo) E receptor-2 (apoER2) 1 is a member of the low density lipoprotein (LDL) receptor gene family and a prototype receptor that acts both in endocytosis and in signal transduction (1-4). In particular, apoER2 functions as a cellular receptor for Reelin, a signaling factor that regulates neuronal migration processes in the embryonic brain. Reelin binds to apoER2 and to the related very low density lipoprotein receptor on the surface of post-mitotic neurons that migrate to their proper position in the developing brain (2, 5). Binding of Reelin results in tyrosine phosphorylation of Disabled 1, an adaptor protein bound to the receptor tails, and in activation of downstream signaling pathways involving the Src family of tyrosine kinases and phosphatidylinositol 3-kinase (4, 6 -8). Defects in these signaling cascades as in apoER-2-deficient mice cause abnormal layering of neurons in the cortex, hippocampus, and cerebellum (9).Aside from post-mitotic neurons in the brain, apoer2 transcripts are also abundant in the placenta, the ovaries, and the epididymis (1, 3, 10). The function of the receptor in these tissues, however, remains unclear. In the present study, we aimed at elucidating novel roles for apoER2 in tissues other than the brain. We focused our attention on the male reproductive system because apoER2 is highly expressed in the principal cells of the epididymis (3) and because receptor-deficient mice suffer from male infertility (9). Here we have identified a crucial role for the receptor in sperm maturation, in particular in the acquisition and development of sperm motility. EXPERIMENTAL PROCEDURESMaterials-The generation of apoER2-deficient mice has been described before (9). Because of male infertility, the line was bred in-house by mating of homozygous-deficient (apoer2 Ϫ/Ϫ ) females with males heterozygous for the receptor gene defect (apoer2 ϩ/Ϫ )...

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Lipid peroxidation in human spermatozoa as relatd to midpiece abnormalities and motility

Cited by 291 publications

References 16 publications

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Vitamin C and oxidative stress in the seminiferous epithelium

Essential Role of the Apolipoprotein E Receptor-2 in Sperm Development

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