Lipid peroxidation caused by oxygen radicals from <i>Fusobacterium</i>‐stimulated neutrophils as a possible model for the emergence of periodontitis

Sheikhi, Maryam; Bouhafs, Rabea K.L.; Hammarström, K-J; Jarstrand, Connie

doi:10.1034/j.1601-0825.2001.70109.x

Cited by 58 publications

(41 citation statements)

References 24 publications

(27 reference statements)

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“…These data suggest that, in periodontal disease, a local increase in the lipid peroxidation level against bacterial products is more prominent than the systemic increase. One of the possible explanations for increased MDA concentration in GCF of periodontitis patients observed in this investigation might be the increase in ROS production during the interaction of bacterial products and neutrophils . Consequently, the change of oxidation‐reduction condition could activate nuclear factor‐ĸB, which in turn triggers the production and activation of proinflammatory cytokines causing more periodontal tissue destruction .…”

Section: Discussionmentioning

confidence: 83%

Malondialdehyde, superoxide dismutase and melatonin levels in gingival crevicular fluid of aggressive and chronic periodontitis patients

Ghallab

Hamdy

Shaker

2016

Australian Dental Journal

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Background: The aim of this study was to investigate malondialdehyde (MDA), superoxide dismutase (SOD) and melatonin levels in the gingival crevicular fluid (GCF) of patients with chronic periodontitis (CP) and generalized aggressive periodontitis (GAgP) as biomarkers for oxidative stress. Methods: The study comprised 65 subjects: 15 healthy individuals, 25 CP patients and 25 GAgP patients. Plaque index, gingival index, pocket depth, clinical attachment level measurements and GCF samples were obtained from all subjects. MDA, SOD and melatonin levels were determined utilizing enzyme-linked immunosorbent assay. Results: GCF-MDA levels were significantly higher in the GAgP group compared to the CP and control groups (p < 0.001) and significantly higher in the CP group than the C group (p < 0.001). SOD and melatonin GCF levels were significantly higher in the control than the GAgP and CP groups (p < 0.05), and significantly lower in the GAgP than the CP group (p < 0.05). The CP group demonstrated a significant negative correlation between GCF-MDA and melatonin concentrations. A positive correlation was observed between SOD and CAL in the CP group and PD in the GAgP group. Conclusions: MDA, melatonin and SOD could be considered as biomarkers for oxidative stress in periodontal diseases and might be useful diagnostic aids in distinguishing CP and GAgP patients.

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Section: Discussionmentioning

confidence: 83%

Malondialdehyde, superoxide dismutase and melatonin levels in gingival crevicular fluid of aggressive and chronic periodontitis patients

Ghallab

Hamdy

Shaker

2016

Australian Dental Journal

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“…Further studies have shown that several isolates of two strains of fusobacteria ( F. nucleatum and F. necrophorum ) can stimulate significant ROS generation (in the presence of plasma), cytokine [interleukin‐1 β (IL‐1 β ), TNF‐ α , IL‐8] and elastase production by neutrophils isolated from healthy individuals (378). This group has also shown that F. nucleatum , in the absence of plasma, could also stimulate large amounts of ROS production and induce lipid peroxidation in vitro (379). That F. nucleatum might be pivotal in neutrophil‐dependent, ROS‐induced tissue damage within the periodontium is also supported by the finding that phagocytosis of F. nucleatum induces significantly greater ROS generation than phagocytosis of Porphyromonas gingivalis or A. actinomycetemcomitans (219).…”

Section: Evidence For the Presence And Role Of Ros In Periodontal Tismentioning

confidence: 99%

The role of reactive oxygen and antioxidant species in periodontal tissue destruction

Chapple

Matthews²

2007

Periodontology 2000

728

799

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“…Thereby the generation of extracellular reactive oxygen species (ROS) predominantly from PMNs is thought to be one of the major factors in periodontal disease pathogenesis [5,6]. Tissue damage in inflammatory periodontal pathologies can be mediated by ROS resulting from the physiological activity of polymorphonuclear leukocytes during phagocytosis of periodontopathic bacteria, and can occur through a number of mechanisms such as protein disruption [7], lipid peroxidation [8,9], induction of proinflammatory cytokines [6] and DNA damage [10].…”

Section: Introductionmentioning

confidence: 99%

Increased Salivary Levels of 8-Hydroxydeoxyguanosine May Be a Marker for Disease Activity for Periodontitis

2012

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Background: 8-hydroxydeoxyguanosine (8-OHdG) is commonly used as a marker to evaluate oxidative DNA damage in disorders including chronic inflammatory diseases such as inflammatory periodontal pathologies. In the current study we hypothesized that the level of 8-OHdG in saliva increases by the periodontal destruction severity determined by clinical parameters as clinical attachment level (CAL). Materials and methods: A cross-sectional study was conducted on a sum of 60 age gender balanced; chronic periodontitis (CP) (n = 20), chronic gingivitis (CG) (n = 20) and healthy (H) (n = 20) individuals. Clinical periodontal parameters and salivary 8-OHdG levels were evaluated. Results: The mean 8-OHdG level in the saliva of the CP group was significantly higher than H and CG groups (p < 0.001). Statistically significant correlation was only observed between the salivary levels of 8-OHdG and age (p < 0.05), probing depth (PD) and CAL (p < 0.001) in CP group. However, when CP patients were classified according to their CAL levels (CAL≥ 3 mm (n = 11) and CAL<3 mm (n = 9)) statistically significant correlation was only observed between the salivary levels of 8-OHdG and CAL≥ 3 mm patients (p < 0.001). Conclusion: We suggest that elevated salivary levels of 8-OHdG may be a marker for disease activity and it may reflect indirectly disease severity parameters such as CAL.

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Lipid peroxidation caused by oxygen radicals from Fusobacterium‐stimulated neutrophils as a possible model for the emergence of periodontitis

Abstract: In periodontitis local bacteria might stimulate PMN to release ROS, which cause inflammation and destruction.

Cited by 58 publications

References 24 publications

Malondialdehyde, superoxide dismutase and melatonin levels in gingival crevicular fluid of aggressive and chronic periodontitis patients

Malondialdehyde, superoxide dismutase and melatonin levels in gingival crevicular fluid of aggressive and chronic periodontitis patients

The role of reactive oxygen and antioxidant species in periodontal tissue destruction

Increased Salivary Levels of 8-Hydroxydeoxyguanosine May Be a Marker for Disease Activity for Periodontitis

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