Lipid Peroxidation by-Products and the Metabolic Syndrome

Pillon, Nicolas; Soulage, Christophe O.

doi:10.5772/46019

Cited by 8 publications

(5 citation statements)

References 137 publications

(164 reference statements)

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“…The aldehyde levels in all models decreased in the order: MDA > HHE > HNE. This is in accordance with the levels detected in human plasma samples, as reviewed by Pillon and Soulage, 70 with MDA being found at the highest concentration, followed by HHE and HNE. Control samples without oil were run in parallel with the oil samples, see Table 5.…”

Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilsupporting

confidence: 91%

“…in DNA), makes α,β-unsaturated aldehydes quite genotoxic. 29,72 HHE and HNE are known to be cytotoxic to cells, and in the review by Pillon and Soulage, 70 LC 50 levels of 20-60 µM after a long incubation time were reported for various animal and human cells; while LC 50 for MDA was reported to be ≥600 µM. 70 These levels are higher than the levels reported in this study at 180 min digestion; the closest are the levels of HHE found in the human digestion model (9.8 µM).…”

Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilmentioning

confidence: 99%

“…29,72 HHE and HNE are known to be cytotoxic to cells, and in the review by Pillon and Soulage, 70 LC 50 levels of 20-60 µM after a long incubation time were reported for various animal and human cells; while LC 50 for MDA was reported to be ≥600 µM. 70 These levels are higher than the levels reported in this study at 180 min digestion; the closest are the levels of HHE found in the human digestion model (9.8 µM). Still, in vitro cellular damage has been reported at lower levels, a 70% inhibition of the cell growth and induced apoptosis was for example observed by Cerbone et al 73 using human Caco-2 cells and HNE (1 µM).…”

Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilmentioning

confidence: 99%

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Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcinein vitrodigestion models

et al. 2016

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In this work, we investigated lipid oxidation of cod liver oil during gastrointestinal (GI) digestion using two types of in vitro digestion models. In the first type of model, we used human GI juices, while we used digestive enzymes and bile from porcine origin in the second type of model. Human and porcine models were matched with respect to factors important for lipolysis, using a standardized digestion protocol. The digests were analysed for reactive oxidation products: malondialdehyde (MDA), 4-hydroxy-trans-2-nonenal (HNE), and 4-hydroxy-trans-2-hexenal (HHE) by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS), and for free fatty acids (FFA) obtained during the digestion by gas chromatography-mass spectrometry (GC-MS). The formation of the oxidation products MDA, HHE, and HNE was low during the gastric digestion, however, it increased during the duodenal digestion. The formation of the oxidation products reached higher levels when digestive juices of human origin were used (60 μM of MDA, 0.96 μM of HHE, and 1.6 μM of HNE) compared to when using enzymes and bile of porcine origin (9.8, and 0.36 μM of MDA; 0.16, and 0.026 μM of HHE; 0.23, and 0.005 μM of HNE, respectively, in porcine models I and II). In all models, FFA release was only detected during the intestinal step, and reached up to 31% of total fatty acids (FA). The findings in this work may be of importance when designing oxidation oriented lipid digestion studies.

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Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilsupporting

confidence: 91%

Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilmentioning

confidence: 99%

Section: Lipolysis During In Vitro Digestion Of Cod Liver Oilmentioning

confidence: 99%

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Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcinein vitrodigestion models

et al. 2016

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“…Peningkatan ROS dalam sel adiposa dapat menyebabkan keseimbangan reaksi reduksi oksidasi terganggu sehingga mengakibatkan penurunan enzim antioksidan di dalam sirkulasi. 26 Selain itu, kondisi hiperglikemia pada penderita sindrom metabolik juga dapat menginduksi stres oksidatif. Peningkatan glukosa darah dapat menghasilkan ketidakseimbangan reaksi oksidasireduksi dalam hepatosit.…”

Section: Pembahasanunclassified

“…Malondialdehyde (MDA) merupakan produk akhir dari degradasi oksidatif non-enzimatik PUFA, yang telah lama digunakan sebagai tanda peroksidasi lipid. 26,27 Kadar MDA yang lebih tinggi dari kondisi normal diketahui dapat terjadi pada individu yang menderita hiperglikemia atau diabetes dan hiperkolesterolemia. 28,29 Sejalan dengan teori tersebut, penelitian ini menunjukkan bahwa kadar MDA pada kelompok K+ (8,7800±0,33724 nmol/ml) lebih tinggi dibandingkan dengan K-(2,3029±0,20766 nmol/ml).…”

Section: Pembahasanunclassified

PENGARUH PEMBERIAN PISANG KEPOK (Musa Paradisiaca Forma Typical) TERHADAP KADAR MALONDIALDEHYDE (MDA) TIKUS SPRAGUE DAWLEY PRA-SINDROM METABOLIK

A.P

Syauqy

2015

J. NutriColl

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Latar Belakang : Peningkatan prevalensi sindrom metabolik berkontribusi terhadap peningkatan kejadian penyakit kardiovaskular dan diabetes. Kondisi dislipidemia dan hiperglikemia yang terjadi pada penderita sindrom metabolik mengindikasikan adanya akumulasi radikal bebas. Peningkatan radikal bebas dan penurunan mekanisme pertahanan antioksidan menghasilkan peningkatan derajat stres oksidatif, yang dapat digambarkan dengan kadar MDA. Buah pisang kepok mengandung antioksidan, vitamin, serat, inulin, dan pati resisten yang berpotensi menurunkan kadar MDA melalui pencegahan oksidasi lipid. Penelitian ini bertujuan untuk mengetahui pengaruh buah pisang kepok terhadap kadar MDA hati pada tikus pra-sindrom metabolik.Metode : Jenis penelitian ini adalah eksperimental dengan post-test only with randomized control group design. Sebanyak 28 ekor tikus Sprague Dawley pra-sindrom metabolik dibagi secara acak menjadi 4 kelompok: K- (hanya diberikan pakan standar), K+ (pakan standard dan induksi STZ(Streptozotocin) dan NAD (nicotinamide)), serta P1, P2 (diberikan pakan standar, induksi STZ dan NAD, serta pisang kepok dengan dosis 4,5g/200gBB dan 9g/200gBB selama 21 hari). Pengukuran MDA hati dilakukan dengan metode TBARS (2-ThioBarbituric Acid Reactive Substance). Data dianalisis dengan uji Anova dan post-hoc LSD.Hasil : Terdapat perbedaan kadar MDA antara K-, K+, P1, P2 (p=0,000). Kadar MDA P2 (3,4457±0,27184 nmol/ml) lebih rendah daripada K+ (8,7800±0,33724 nmol/ml) dan P1 (6,0243±0,50695 nmol/ml), tetapi lebih tinggi daripada K- (2,3029±0,20766 nmol/ml). Hasil uji post-hoc LSD tentang perbedaan kadar MDA antarkelompok menunjukkan signifikansi sebesar p=0,000.Simpulan : Pemberian buah pisang kepok selama 21 hari pada dosis 9g/200gBB/hari dapat membuat kadar MDA pada tikus pra-sindrom metabolik menjadi lebih rendah.

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Oxidative Stress Reduction (Prong-3)

DePace

Colombo²

2019

Clinical Autonomic and Mitochondrial Disorders

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Lipid Peroxidation by-Products and the Metabolic Syndrome

Cited by 8 publications

References 137 publications

Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcinein vitrodigestion models

Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcinein vitrodigestion models

PENGARUH PEMBERIAN PISANG KEPOK (Musa Paradisiaca Forma Typical) TERHADAP KADAR MALONDIALDEHYDE (MDA) TIKUS SPRAGUE DAWLEY PRA-SINDROM METABOLIK

Oxidative Stress Reduction (Prong-3)

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