Lipid peroxidation assessment by malondialdehyde measurement in parenteral nutrition solutions for newborn infants: a pilot study

JC, Picaud; JP, Steghens; Auxenfans, Céline; Barbieux, A; Laborie, S; O, Claris

doi:10.1080/08035250310022324

Cited by 6 publications

(5 citation statements)

References 18 publications

(31 reference statements)

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“…We first analyzed malondialdehyde (MDA), a secondary metabolite resulting from lipid peroxidation by ROS, reflecting oxidative damage levels (Picaud et al, 2004 ). Differences in MDA levels were significant for 8 out of total 15 factors and between-factors interactions (Table 2 ), demonstrating the impact of drought (significant D), and also demonstrating that the different regions of the growth zone (significant L), and maize lines from different geographic origin (significant O), experienced varying extents of lipid peroxidation.…”

Section: Resultsmentioning

confidence: 99%

High Antioxidant Activity Facilitates Maintenance of Cell Division in Leaves of Drought Tolerant Maize Hybrids

et al. 2017

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We studied the impact of drought on growth regulation in leaves of 13 maize varieties with different drought sensitivity and geographic origins (Western Europe, Egypt, South Africa) and the inbred line B73. Combining kinematic analysis of the maize leaf growth zone with biochemical measurements at a high spatial resolution allowed us to examine the correlation between the regulation of the cellular processes cell division and elongation, and the molecular redox-regulation in response to drought. Moreover, we demonstrated differences in the response of the maize lines to mild and severe levels of water deficit. Kinematic analysis indicated that drought tolerant lines experienced less impact on leaf elongation rate due to a smaller reduction of cell production, which, in turn, was due to a smaller decrease of meristem size and number of cells in the leaf meristem. Clear differences in growth responses between the groups of lines with different geographic origin were observed in response to drought. The difference in drought tolerance between the Egyptian hybrids was significantly larger than between the European and South-African hybrids. Through biochemical analyses, we investigated whether antioxidant activity in the growth zone, contributes to the drought sensitivity differences. We used a hierarchical clustering to visualize the patterns of lipid peroxidation, H2O2 and antioxidant concentrations, and enzyme activities throughout the growth zone, in response to stress. The results showed that the lines with different geographic region used different molecular strategies to cope with the stress, with the Egyptian hybrids responding more at the metabolite level and African and the European hybrids at the enzyme level. However, drought tolerance correlated with both, higher antioxidant levels throughout the growth zone and higher activities of the redox-regulating enzymes CAT, POX, APX, and GR specifically in leaf meristems. These findings provide evidence for a link between antioxidant regulation in the leaf meristem, cell division, and drought tolerance.

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Section: Resultsmentioning

confidence: 99%

High Antioxidant Activity Facilitates Maintenance of Cell Division in Leaves of Drought Tolerant Maize Hybrids

et al. 2017

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“…The method to assess MDA-Hb is an adaptation of a reliable and validated method [10] used to evaluate the impact of parenteral nutrition composition on lipid peroxidation [18–20]. MDA-Hb was measured by LC-MS using a very specific method based on diaminonaphthalene derivatization [10].…”

Section: Discussionmentioning

confidence: 99%

Malondialdehyde Adduct to Hemoglobin: A New Marker of Oxidative Stress Suitable for Full-Term and Preterm Neonates

Cipierre

Hays

Maucort-Boulch

et al. 2013

Oxidative Medicine and Cellular Longevity

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Oxidative stress may play a central role in the onset of many diseases during the neonatal period. Malondialdehyde (MDA) is a marker of lipid peroxidation. The aim of this study was to evaluate a new marker, the malondialdehyde adduct to hemoglobin (MDA-Hb), which is measured in red blood cells (RBCs) and thus does not require that an additional blood sample be drawn. In this prospective study, we first adapted the measurement method previously described to Hb solutions obtained from washed RBCs and then evaluated the suitability of the method for use in neonates. MDA-Hb concentrations were measured by liquid chromatography-mass spectrometry. We compared the concentrations of MDA-Hb between preterm and term neonates. Erythrocyte samples were collected at birth from 60 healthy neonates (29 full-term and 31 preterm), as well as from 50 preterm neonates with uncomplicated postnatal evolution during the first months of life. We found a significantly higher MDA-Hb concentration at birth in preterm neonates (P = 0.002). During the first months of life, MDA-Hb concentrations were 9.4 nanomol/g Hb in hospitalized preterm neonates. MDA-Hb could be used to assess oxidative stress in preterm neonates. Together with clinical variables, it could be a useful marker for oxidative stress exposition in these higher risk patients.

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“…MDA and HNE have been used as markers for lipid peroxidation. [ 76 77 78 ] MDA can react with amino acids in proteins and other molecules to form its adducts such as malondialdehyde-acetaldehyde and advanced lipid peroxidation end-products, which can produce secondary deleterious effects by promoting intra- or inter-molecular protein or DNA crosslinking to cause protein modification and DNA damage/mutation. [ 79 80 81 82 83 ] 4-HNE is a very reactive compound with three reactive groups: an aldehyde, a double-bond, and a hydroxyl group.…”

Section: Functions Of Reactive Oxygen Species In Ischemic Strokementioning

confidence: 99%

Targeting oxidative stress for the treatment of ischemic stroke: Upstream and downstream therapeutic strategies

Yang

2016

Brain Circ

102

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Excessive oxygen and its chemical derivatives, namely reactive oxygen species (ROS), produce oxidative stress that has been known to lead to cell injury in ischemic stroke. ROS can damage macromolecules such as proteins and lipids and leads to cell autophagy, apoptosis, and necrosis to the cells. This review describes studies on the generation of ROS, its role in the pathogenesis of ischemic stroke, and recent development in therapeutic strategies in reducing oxidative stress after ischemic stroke.

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Lipid peroxidation assessment by malondialdehyde measurement in parenteral nutrition solutions for newborn infants: a pilot study

Cited by 6 publications

References 18 publications

High Antioxidant Activity Facilitates Maintenance of Cell Division in Leaves of Drought Tolerant Maize Hybrids

High Antioxidant Activity Facilitates Maintenance of Cell Division in Leaves of Drought Tolerant Maize Hybrids

Malondialdehyde Adduct to Hemoglobin: A New Marker of Oxidative Stress Suitable for Full-Term and Preterm Neonates

Targeting oxidative stress for the treatment of ischemic stroke: Upstream and downstream therapeutic strategies

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